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In vitro studies in Tagetes erecta (marigold) under auxins (IAA, NAA) and cytokinins (BAP, Kinetin) effect for callus formation by different explants

TL;DR: Ex-plants of Tagetes erecta were subjected to different concentration of auxin (IAA, NAA) and cytokinin (BAP, Kinetin) to observe their response towards callus formation.
Abstract: Culture establishment depends on its physical and chemical environment. Different ex-plants of Tagetes erecta were subjected to different concentration of auxin (IAA, NAA) and cytokinin (BAP, Kinetin) to observe their response towards callus formation. Best callus formation was seen in loose cells of leaf blade as compared to arranged cells of nodes.
Citations
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Journal ArticleDOI
TL;DR: This review includes reports on pharmacological aspects like antibacterial, antifungal, larvicidal, hepatoprotective, insecticidal, mosquitocidal, nematicidal, wound healing, antioxidant, anticancer and antidiabetic properties/activity of Tagetes.
Abstract: Importance of medicinal plants to health care has been great and herbal preparations are being produced at industrial scale particularly in developing countries. The plant products obtained have a long history of use in therapeutics, aromatherapy and food depending on the chemical constituents and their bioactivity. In the recent past, marigolds have received a great attention in scientific research, because of their multiple use and also the information available about their phytochemistry and bioactivity. Tagetes species commonly known as marigold is native to Mexico, being used for medicinal and ornamental purposes. The plant is useful due to its unique phytoconstituents for a range of diseases and disorders and is reportedly effective against piles, kidney troubles, muscular pain, ulcers and wound healing and the flowers are helpful in fever, stomach and liver complaints and also in eye diseases. In India, marigold is also extensively used on religious and social occasions such as in the beautification of mandaps and pooja places; offerings at temples; marriage decorations and landscape planning due to variable size and colour of its flower. Present review is an effort to bring together the different strategies developed for the growth and cultivation of marigold, its ecophysiological and remediation relevance under a variety of environmental conditions and possible allelopathic potential. It includes reports on pharmacological aspects like antibacterial, antifungal, larvicidal, hepatoprotective, insecticidal, mosquitocidal, nematicidal, wound healing, antioxidant, anticancer and antidiabetic properties/activity of Tagetes.

16 citations


Cites background from "In vitro studies in Tagetes erecta ..."

  • ...102024 324 American Journal of Plant Sciences arranged cells of nodes [197]....

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Journal ArticleDOI
TL;DR: The result revealed that growth regulators IBA and NAA had significantly effect on shooting performance of African marigold.
Abstract: The experiment was carried out in completely randomized design with three replications and the cuttings were planted on protray and placed at mist chamber and consist of three different concentrations of IBA (100, 200 and 300 ppm), NAA (100, 200 and 300ppm) and IBA+NAA (100+100, 200+200 and 300+300 ppm) along with control were treated for shooting in stem cuttings of marigold under Chhattisgarh plain region. The result revealed that growth regulators IBA and NAA had significantly effect on shooting performance of African marigold. The number of sprouts per cutting (7.55), average length of shoot (8.00 cm), number of leaves per cutting (163.44), fresh weight (1140.55 mg) and dry weight of shoot (159.00 mg) were recorded maximum in NAA 200 ppm at 35 days after planting of cuttings.

3 citations


Cites background from "In vitro studies in Tagetes erecta ..."

  • ...com JPP 2020; 9(3): 1460-1461 Received: 18-03-2020 Accepted: 23-04-2020...

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  • ...~ 1460 ~ Journal of Pharmacognosy and Phytochemistry 2020; 9(3): 1460-1461...

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01 Jan 2014
TL;DR: Till date, high growth of callus, shoot and root formation is observed in many of the research works and applications of newer methods are being employed so as to minimize the cost with an increase in the resultant outcome.
Abstract: The emerging new techniques in Plant Tissue Culture have led to the rapid growth of many plants of importance. Seasonal plants are even grown throughout the year under aseptic conditions. Plants of high medicinal value are cultivated in bulk and helps in increased amount for extraction of the necessary compounds. Tagetes erecta , a herbal medicinal plant, is propagated in vitro by applying the techniques of plant tissue culture. Till date, high growth of callus, shoot and root formation is observed in many of the research works. Also other species of the Tagetes family have shown good outcome in developing callus and root through tissue culture methods. Great work has been done in this field for the growth of the medicinal plant and many more are even expected to come. Applications of newer methods are being employed so as to minimize the cost with an increase in the resultant outcome.

2 citations

Journal Article
TL;DR: In this article, young tender leaves of T.erecta were collected from plants grown in pots under adequate sunlight and rainfall and inoculated in MS medium supplemented with Kn, IAA, NAA and 2-4-D in different combinations.
Abstract: Tagetes erecta is a plant of great medicinal value and is in high commercial demand due to its various uses in religious purposes, as eatables, and is used for the treatment of headache and strengthening of the heart, treating wounds and prevent them from getting infected with bacteria and toxins. Young tender leaves of T.erecta were collected from plants grown in pots under adequate sunlight and rainfall.. Leaves were sterilised with 70% ethanol for 5 minutes and 0.02% Mercuric chloride for 3 minutes and inoculated in MS medium supplemented with Kn, IAA, NAA and 2,4-D in different combinations (0, 0.5, 2.5, 5.0, 10 mg/L). 50% callus formation are observed in 0.5 mg/L Kn from LT, 0.5 mg/L Kn + 2.5 mg/L IAA from LM, 0.5 mg/L Kn + 10.0 mg/L IAA from LM, 2.5 mg/L Kn + 0.5 mg/L IAA from LT, 5.0 mg/L Kn + 2.5 mg/L 2,4-D from LT and 10.0 mg/L Kn + 0.5 mg/L IAA from LM. 100% callus formation occurred in the combinations of 0.5 mg/L Kn + 5.0 mg/L 2,4-D from LB and LM, 2.5 mg/L Kn from LT and 5.0 mg/L Kn + 0.5 mg/L IAA from LT. Root formation was observed in the concentration of 2.5 mg/L Kn + 0.5 mg/L IAA from LT. Root formation was observed in low concentration of Kn in combination with a low concentration of cytokinin. Callus induction resulted in high concentration of Kn and cytokinin.. It has also been observed that the explants responded differently towards different growth regulators in varying concentrations. The best callus growth was found using the Kn and IAA as growth regulators within a short time period.

2 citations


Additional excerpts

  • ...Int J Pharm Bio Sci 2014 April ; 5 (2) : (B) 319 - 332...

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Journal ArticleDOI
TL;DR: In this article, the effect of IBA and NAA and their combination on rooting in stem cuttings of African Marigold (Tagetes erecta L.) cv. was conducted in the Horticultural Research cum Instructional Farm at Department of Floriculture and Landscape Architecture, College of Agriculture, Indira Gandhi Krishi Vishwavidyalya, Raipur (C. G.), season 2017.
Abstract: The present investigation entitled “Effect of IBA and NAA and their combination on rooting in stem cuttings of African Marigold (Tagetes erecta L.) cv. Pusa Narangi Gainda” was conducted in the Horticultural Research cum Instructional Farm at Department of Floriculture and Landscape Architecture, College of Agriculture, Indira Gandhi Krishi Vishwavidyalya, Raipur (C. G.), season 2017. The experiment consisted of three different concentrations of IBA (100, 200 and 300 ppm), NAA (100, 200 and 300ppm) and IBA+NAA (100+100, 200+200 and 300+300 ppm) along with control which were treated for root initiation in stem cutting of marigold under Chhattisgarh plain region. The experiment was laid out in a Completely Randomized Design with three replications and the cuttings were planted on protray and placed in mist chamber. The result revealed that growth regulators IBA and NAA had significantly effect on rooting and shooting performance of marigold. The maximum survival percentage (88.33%) of rooted cuttings were recorded under 200 ppm IBA and 300 ppm IBA at 35 days after planting of cuttings. While the number of sprouts per cutting (7.55), average length of shoot (8.00 cm), number of leaves per cutting (163.44), fresh weight (1140.55 mg) and dry weight of shoot (159.00 mg) were recorded maximum in NAA 200 ppm at 35 days after planting of cuttings and the number of roots (93.44), fresh weight (404 mg) and dry weight of root (34.11 mg) were recorded maximum under 300 ppm IBA whereas, average length of roots (12.23 cm) were observed maximum under IBA 100 ppm.

2 citations

References
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Journal ArticleDOI
TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
Abstract: In experiments with tobacco tissue cultured on White's modified medium (basal meditmi hi Tnhles 1 and 2) supplemenk'd with kiticthi and hidoleacctic acid, a slrikin^' fourlo (ive-told intTease iu yield was ohtaitu-d within a three to Tour week j^rowth period on addition of an aqtteotis exlrarl of tobacco leaves (Fi^'ures 1 and 2). Subse(iueutly it was found Ihiit this jnoniotiou oi' f^rowih was due mainly though nol entirely to inorj^auic rather than organic con.stitttenls in the extract. In the isolation of Rrowth factors from plant tissues and other sources inorj '̂anic salts are fre(|uently carried along with fhe organic fraclioits. When tissue cultures are used for bioassays, therefore, il is necessary lo lake into account increases in growth which may result from nutrient elements or other known constituents of the medium which may he present in the te.st materials. To minimize interference trom rontaminaitis of this type, an altempt has heen made to de\\eh)p a nieditmi with such adequate supplies of all re(iuired tnineral nutrients and cotntnott orgattic cottslitueitls that no apprecial»le change in growth rate or yield will result from the inlroduclion of additional amounts in the range ordinarily expected to be present in tnaterials to be assayed. As a point of referetice for this work some of the culture media in mc)st common current use will he cotisidered briefly. For ease of comparis4)n Iheir mineral compositions are listed in Tables 1 and 2. White's nutrient .solution, designed originally for excised root cultures, was based on Uspeuski and Uspetiskaia's medium for algae and Trelease and Trelease's micronutrieni solution. This medium also was employed successfully in the original cttltivation of callus from the tobacco Iiybrid Nicotiana gtauca x A', tanijadorffii, atitl as further modified by White in 194̂ ^ and by others it has been used for the

63,098 citations

01 Jan 1962

16,251 citations


"In vitro studies in Tagetes erecta ..." refers methods in this paper

  • ...MS medium (Murashige & Skoog, 1962) was used throughout the study supplemented with different growth hormones....

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Book ChapterDOI
TL;DR: This chapter describes the method developed in the laboratory that has undergone further refinements and involves Agrobacterium-mediated transformation of cotton cells, selection of stable transgenic callus lines, and recovery of plants via somatic embryogenesis.
Abstract: Cotton continues to be a crop of great economic importance in many developing and some developed countries. Cotton plants expressing the Bt gene to deter some of the major pests have been enthusiastically and widely accepted by the farmers in three of the major producing countries, i.e., China, India, and the USA. Considering the constraints related to its production and the wide variety of products derived from the cotton plant, it offers several target traits that can be improved through genetic engineering. Thus, there is a great need to accelerate the application of biotechnological tools for cotton improvement. This requires a simple, yet robust gene delivery/transformant recovery system. Recently, a protocol, involving large-scale, mechanical isolation of embryonic axes from germinating cottonseeds followed by direct transformation of the meristematic cells has been developed by an industrial laboratory. However, complexity of the mechanical device and the patent restrictions are likely to keep this method out of reach of most academic laboratories. In this chapter, we describe the method developed in our laboratory that has undergone further refinements and involves Agrobacterium-mediated transformation of cotton cells, selection of stable transgenic callus lines, and recovery of plants via somatic embryogenesis.

111 citations

Journal ArticleDOI
TL;DR: An efficient and reproducible protocol for mass propagation of Eclipta alba (L.) Hassk, an important medicinal plant, was standardized by culturing shoot tips and nodal segments taken from in vitro raised plants.
Abstract: An efficient and reproducible protocol for mass propagation of Eclipta alba (L.) Hassk, an important medicinal plant, was standardized by culturing shoot tips and nodal segments taken from in vitro raised plants. Maximum shoot proliferation occurred when the explants were cultured on Murashige and Skoog (MS) medium supplemented with 1 mg l−1 benzylaminopurine (BAP). The shoot buds formed were further multiplied and maintained on medium containing BAP (0.5 mg l−1) and gibberellic acid (0.5 mg l−1). Rooting was best achieved on MS medium supplemented with 1 mg−1 indole-3-butyric acid. Rooted plantlets attained maturity and flowered normally in the field.

57 citations

Journal ArticleDOI
TL;DR: Thirteen days was the best time of exposure of the explant to the regeneration medium for shoot induction and the highest values for regeneration were obtained with BA (13.3 μM) and IAA (17.1 μM).
Abstract: Regeneration of whole plants of marigold (Tagetes erecta L.) was achieved by organogenesis using leaf explants. Leaf segments about 0.25 cm 2 were taken from 3-week-old in vitro plantlets and cultured on MS basal medium containing BA with different auxins (NAA, 2,4-D and IAA). The exposure time of the explants on the regeneration medium was tested. The highest values for regeneration were obtained with BA (13.3 µM) and IAA (17.1 µM). Thirteen days was the best time of exposure of the explant to the regeneration medium for shoot induction. Abbreviations: BA – 6-benzylaminopurine; 2,4-D – 2,4-dichlorophenoxyacetic acid; IAA – indole-3-acetic acid; MS – Murashige and Skoog basal medium; NAA – α-naphthaleneacetic acid; BFC – Bud forming capacity

56 citations