In Vitro study of Vellozia pusilla pohl (Velloziaceae), a Brazilian plant species: antitumoral activity and labeling of blood elements
Ana Letícia Almeida Dantas,Ana Letícia Almeida Dantas,Ligia M. M. Valente,Lilia Aparecida Salgado de Morais,Gláucio Diré Feliciano,Mario Bernardo-Filho +5 more
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TLDR
The methanol extract of roots, stem and leaf sheaths of this species showed an antitumoral activity through the inhibition of the enzyme Topoisomerase I when analyzed by an in vitro bioassay employing DNA repair or recombination deficient mutants of the yeast Saccharomyces cerevisiae.Abstract:
Vellozia pusilla Pohl is a species of the botanic family Velloziaceae that occurs in the subtropical regions of South America and, although it lives under conditions of high solar irradiation and low water availability, shows great longevity. The methanol extract of roots, stem and leaf sheaths of this species showed an antitumoral activity through the inhibition of the enzyme Topoisomerase I when analyzed by an in vitro bioassay employing DNA repair or recombination deficient mutants of the yeast Saccharomyces cerevisiae. In the evaluation of the effect of Vellozia pusilla methanol extract on the labeling of RBC, blood of mice was treated with 99mTc tracer solutions. The percentage of radioactivity (%ATI) bound to plasma (P) and blood cells (BC) was determined. The %ATI in the insoluble fraction of plasma (IF) was also evaluate, and the results showed that there was a decrease in %ATI in this fraction that represents the plasmatic proteins.read more
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Structure Elucidation, Antimicrobial and Cytotoxic Activities of a Halimane Isolated from Vellozia kolbekii Alves (Velloziaceae).
Carmelita Gomes da Silva,Helvécio M. Santos Júnior,Jussara P. Barbosa,Gisela Lara da Costa,Felipe A. R. Rodrigues,Denilson F. Oliveira,Letícia V. Costa-Lotufo,Ruy José Válka Alves,Elis C. A. Eleutherio,Claudia de Moraes Rezende +9 more
TL;DR: In the mechanism of cytotoxic action, halimane 1 interferes in two major phases of the cell cycle: in S phase, in which DNA synthesis occurs and where it is very sensitive to damage, and G2M phase which is the phase of preparation for mitosis and mitosis itself, showing apoptosis‐inducing properties.
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Uncaria tomentosa extract: evaluation of effects on the in vitro and in vivo labeling of blood constituents with technetium-99m
Silvana Ramos Farias Moreno,Silvana Ramos Farias Moreno,Jorge José de Carvalho,Ana Lucia Rosa Nascimento,Beni Olej,Emely Kazan Rocha,Adriano Arnobio,Mario Bernardo-Filho,Luiz Querino de Araújo Caldas,Hayden Honeycut +9 more
TL;DR: The analysis of the results of the in vivo and in vitro study indicates that there is no significant alteration on the uptake of Tc-99m by the blood constituents, but it significantly decrease the labeling of blood constituents by in vitro methods.
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Effect of the extract of Ricinus communis L. on the osmotic fragility, labeling of red blood cells with Technetium-99m and morphology of the cells
Kristiana Cerqueira Mousinho,Marília B. L. Correia,Jailson Oliveira da Silva,Simey de Souza Leão Pereira Magnata,Ivone Antônia de Souza,Maria Teresa Jansem de Almeida Catanho +5 more
TL;DR: Results suggested that the proteic extract of R. communis changed the capability of binding of the red blood cells due to the stannous ion oxidation, modifying the cells structure.
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Efeito da fração não-oleosa doRicinus communisna biodistribuição comTecnécio-99m em camundongos
K.C. Mousinho,T.J. Matos-Rocha,E.A.C. Pereira,Marília B. L. Correia,S.L.P. Simey,Ivone Antônia de Souza,A. A. Carvalho,Maria Teresa Jansem de Almeida Catanho +7 more
TL;DR: There is antitumor activity in the NOFRC, but that it alters the biodistribution of Tc-99m, a fact which may result in failures in the diagnostic imaging process using this radioisotope as a marker.
References
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Biological and Morphological Aspects of the Velloziaceae
TL;DR: The leaves of Vellozii?
Journal ArticleDOI
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Journal ArticleDOI
Norditerpenoids from Vellozia pusilla
TL;DR: In this article, a series of known di-and triterpenoids from Vellozia pusilla and their structures were elucidated by spectroscopic methods and chemical transformations.