Fig. 3. PCR detection of WSSV in P. monodon challenged with WSSV after oral administration of the mangrove plant extracts. Each lane represents the PCR product of WSSV from shrimp. Lanes 1–6: Shrimp found live after challenge with WSSV subsequent to oral administration of the aqueous extracts of C. tagal, Sonneratia sp., Avicennia sp., E. agallocha, A. ilicifolius and R. apiculata respectively. Lanes 7–11: Shrimp found dead after challenge with WSSSV subsequent to oral administration of the aqueous extracts of E. agallocha, Avicennia sp. A.ilicifolius R. apiculata and R. mucronata. Lane 12: Negative control (animal fed on placebo and without WSSV challenge). Lane 13: Positive control (animal fed on placebo andWSSV challenge). M: Molecular weight marker.
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