In vivo trans-specific gene silencing in fungal cells by in planta expression of a double-stranded RNA
Maria Laine Penha Tinoco,Maria Laine Penha Tinoco,Maria Laine Penha Tinoco,Bárbara B. A. Dias,Rebeca C. D’Allastta,Rebeca C. D’Allastta,João Alencar Pamphile,Francisco J. L. Aragão,Francisco J. L. Aragão,Francisco J. L. Aragão +9 more
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TLDR
This study demonstrates for the first time the in vivo interference phenomenon in the pathogenic fungus Fusarium verticillioides, in which expression of an individual fungal transgene was specifically abolished by inoculating mycelial cells in transgenic tobacco plants engineered to express siRNAs from a dsRNA corresponding to the particular transgenes.Abstract:
Self-complementary RNA transcripts form a double-stranded RNA (dsRNA) that triggers a sequence-specific mRNA degradation, in a process known as RNA interference (RNAi), leading to gene silencing. In vascular plants, RNAi molecules trafficking occur between cells and systemically throughout the plant. RNAi signals can spread systemically throughout a plant, even across graft junctions from transgenic to non-transgenic stocks. There is also a great interest in applying RNAi to pathogenic fungi. Specific inhibition of gene expression by RNAi has been shown to be suitable for a multitude of phytopathogenic filamentous fungi. However, double-stranded (ds)RNA/small interfering (si)RNA silencing effect has not been observed in vivo. This study demonstrates for the first time the in vivo interference phenomenon in the pathogenic fungus Fusarium verticillioides, in which expression of an individual fungal transgene was specifically abolished by inoculating mycelial cells in transgenic tobacco plants engineered to express siRNAs from a dsRNA corresponding to the particular transgene. The results provide a powerful tool for further studies on molecular plant-microbe and symbiotic interactions. From a biotechnological perspective, silencing of fungal genes by generating siRNAs in the host provides a novel strategy for the development of broad fungi-resistance strategies in plants and other organisms.read more
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Host-induced gene silencing of cytochrome P450 lanosterol C14α-demethylase–encoding genes confers strong resistance to Fusarium species
TL;DR: It is demonstrated that host-induced gene silencing (HIGS) targeting the fungal sterol 14α-demethylase (CYP51) genes restricts Fusarium infection in plants, demonstrating that HIGS is a powerful tool, which could revolutionize crop plant protection.
Journal ArticleDOI
Cotton plants export microRNAs to inhibit virulence gene expression in a fungal pathogen
Tao Zhang,Yunlong Zhao,Jianhua Zhao,Sheng Wang,Yun Jin,Zhong-Qi Chen,Yuan-Yuan Fang,Chenlei Hua,Shou-Wei Ding,Hui-Shan Guo +9 more
TL;DR: This work shows that in response to infection with Verticillium dahliae, cotton plants increase production of microRNA 166 and miR159 and export both to the fungal hyphae for specific silencing, identifying a novel defence strategy of host plants by exporting specific miRNAs to induce cross-kingdom gene silencing in pathogenic fungi and confer disease resistance.
Journal ArticleDOI
Intercellular and systemic movement of RNA silencing signals
TL;DR: A review discusses the advances in systemic RNAi and presents the current challenges and questions in this rapidly evolving field.
Journal ArticleDOI
New wind in the sails: improving the agronomic value of crop plants through RNAi-mediated gene silencing
Aline Koch,Karl-Heinz Kogel +1 more
TL;DR: Recent studies that reveal the enormous potential RNAi strategies hold not only for improving the nutritive value and safety of the food supply, but also for providing an environmentally friendly mechanism for plant protection are reviewed.
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Development of a host-induced RNAi system in the wheat stripe rust fungus Puccinia striiformis f. sp. tritici.
TL;DR: The results indicate that an in planta RNAi approach can be used in functional genomics research for rust fungi and that it could potentially be used to engineer durable resistance.
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