Induction of Airway Mucus Production By T Helper 2 (Th2) Cells: A Critical Role For Interleukin 4 In Cell Recruitment But Not Mucus Production
Citations
194 citations
192 citations
Cites background or methods from "Induction of Airway Mucus Productio..."
...This PBS as previously described (4), for 20 min/d for a total of 7 d index was calculated for each mouse lung and then the mean of over a period of 9 d (four consecutive days exposed, 2 d rested, the HMI was calculated for each experimental group....
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...10 CD4 Th1 or Th2 cells were transferred that received IL-13 / or IL-13 / Th2 cells and IL-13 / into recipient mice, we previously showed that Th2, but not mice that received IL-13 / Th2 cells and inhaled OVA Th1, cells stimulate airway epithelial mucus (4)....
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...Using a system in which in vitroor IL-5, and in inflammatory responses that were devoid generated T cell receptor transgenic Th2 cells are transferred of eosinophils or mast cells (4, 5)....
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...Airway mucus staining in IL-4R / bone marrow Th1 cells typically do not stimulate mucus production due chimeras after transfer of Th2 to the inhibitory action of IFN- (4, 16)....
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...murine models of antigen-induced airway inflammation, the IL-13 has a unique in vivo function in gastrointestinal expulinduction of mucus requires the presence of activated CD4 sion of the parasitic nematode, Nippostrongylus braziliensis Th2 cells (4)....
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187 citations
186 citations
Cites background from "Induction of Airway Mucus Productio..."
...IL-4 is critical for Th2 cell induction (35), especially during the sensitization phase (20–22), but it has been shown that in the absence of IL-4, AHR, lung eosinophilia and mucus production can still be induced (36)....
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184 citations
Cites background from "Induction of Airway Mucus Productio..."
...OVA challenge of naive mice receiving transfer of OVA-specific, IL4-deficient Th2 cells resulted in marked mucus hyperproduction in the airways, suggesting that IL-4 was not necessary (56)....
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References
7,567 citations
"Induction of Airway Mucus Productio..." refers background in this paper
...The lower limit of sensitivity for each of the ELISAs was 0.6 ng/ml (IFNg ), 5 pg/ml (IL-4), 0.010 ng/ml (IL-5), and 200 pg/ml (IL-10)....
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...CD4 Th2 cells make a different panel of cytokines, including IL-4, IL-5, and IL-10 (17, 18)....
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...Assays were standardized with recombinant IFNg , IL-5, IL-10 (Endogen), and IL-4 (Collaborative Research, Inc.)....
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...1 A ), and IL-10 (data not shown)....
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...IL-4 2/2 OVA-specific Th2 cells produced comparable levels of IL-5 and IL-10 when compared to IL-4 1/1 OVA-specific Th2 cells, but IL-4 was produced only by IL-4 1/1 Th2 cells....
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2,898 citations
"Induction of Airway Mucus Productio..." refers background in this paper
...Th2 cells secreting IL-4 and IL-5 have been shown to be present and activated in the bronchial wall of asthmatic individuals (9, 23)....
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1,916 citations
"Induction of Airway Mucus Productio..." refers methods in this paper
...To generate Th1 or Th2 cells from DO11.10 mice, CD4 T cells were isolated by negative selection as previously described (31) using mAbs to CD8 (clone 53-6.72, clone 2.43 [ 32 ]), Class II MHC I-A d (212.A1 [33]) and anti‐Ig-coated magnetic beads (Advanced Magnetics, Inc....
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1,831 citations
1,262 citations