Induction of Airway Mucus Production By T Helper 2 (Th2) Cells: A Critical Role For Interleukin 4 In Cell Recruitment But Not Mucus Production
Citations
54 citations
54 citations
54 citations
Cites methods from "Induction of Airway Mucus Productio..."
...10 TCR Tg mice and then transferred into naive wild-type recipients, followed by intranasal OVA challenge to induce inflammatory responses in the lung (22)....
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...Populations of OVA-specific Th2 CD4 T cells were generated in vitro as described by previous investigators (22) using 5 mg/ml pOVA323–339 (GenScript, Piscataway, NJ), 15 ng/ml IL-4 (PeproTech, Rocky Hill, NJ), 25 U/ml IL-2 (Roche Diagnostics, Indianapolis, IN), and 2....
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53 citations
Cites background from "Induction of Airway Mucus Productio..."
...In asthma, allergen-induced Th2 cells in the lungs lead to eosinophilic airway inflammation, increased secretion of mucus and recurring bronchospasm (18)....
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52 citations
References
7,567 citations
"Induction of Airway Mucus Productio..." refers background in this paper
...The lower limit of sensitivity for each of the ELISAs was 0.6 ng/ml (IFNg ), 5 pg/ml (IL-4), 0.010 ng/ml (IL-5), and 200 pg/ml (IL-10)....
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...CD4 Th2 cells make a different panel of cytokines, including IL-4, IL-5, and IL-10 (17, 18)....
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...Assays were standardized with recombinant IFNg , IL-5, IL-10 (Endogen), and IL-4 (Collaborative Research, Inc.)....
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...1 A ), and IL-10 (data not shown)....
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...IL-4 2/2 OVA-specific Th2 cells produced comparable levels of IL-5 and IL-10 when compared to IL-4 1/1 OVA-specific Th2 cells, but IL-4 was produced only by IL-4 1/1 Th2 cells....
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2,898 citations
"Induction of Airway Mucus Productio..." refers background in this paper
...Th2 cells secreting IL-4 and IL-5 have been shown to be present and activated in the bronchial wall of asthmatic individuals (9, 23)....
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1,916 citations
"Induction of Airway Mucus Productio..." refers methods in this paper
...To generate Th1 or Th2 cells from DO11.10 mice, CD4 T cells were isolated by negative selection as previously described (31) using mAbs to CD8 (clone 53-6.72, clone 2.43 [ 32 ]), Class II MHC I-A d (212.A1 [33]) and anti‐Ig-coated magnetic beads (Advanced Magnetics, Inc....
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1,831 citations
1,262 citations