scispace - formally typeset
Search or ask a question
Journal ArticleDOI

Induction of morphogenesis in meristems of different cultivars of Pisum sativum L.

01 Jan 1990-Plant Science (Elsevier)-Vol. 67, Iss: 2, pp 221-226
TL;DR: Variations of morphogenetic behaviour observed in meristem cultures of the investigated pea cultivars manifested a rather strict genetic determination of their regeneration abilities, especially in respect of flowering and rooting of the regenerated shoots.
About: This article is published in Plant Science.The article was published on 1990-01-01. It has received 9 citations till now. The article focuses on the topics: Callus formation & Kinetin.
Citations
More filters
Book
11 Dec 2000
TL;DR: Chemistry nutrition plant physiology, agronomy processing biotechnology breeding strategies for improving grain legume carbohydrates, and research into breeding strategies to improve grain legumes carbohydrates are presented.
Abstract: Chemistry nutrition plant physiology and agronomy processing biotechnology breeding strategies for improving grain legume carbohydrates. (Part contents).

110 citations

Book ChapterDOI
01 Jan 1994
TL;DR: Among the prevalent tissue culture techniques of agricultural and horticultural promise, meristem and shoot tip culture has been exploited at a much wider scale primarily due to its application in diverse areas such as rapid clonal multiplication of vegetatively propagated crop plants, virus elimination and germplasm preservation.
Abstract: Among the prevalent tissue culture techniques of agricultural and horticultural promise, meristem and shoot tip culture has been exploited at a much wider scale primarily due to its application in diverse areas such as rapid clonal multiplication of vegetatively propagated crop plants, virus elimination and germplasm preservation of both vegetatively and seed propagated crops (Kartha, 1981; 1986; George and Sherrington, 1984; Hussey, 1986), and more recently in the development of genetic transformation protocols for crop improvement through gene transfer (Ulian, 1988; Gould et al., 1991).

59 citations

Journal ArticleDOI
TL;DR: Pea (Pisum sativum L. cv. Espace) seeds directly cultured on thidiazuron (TDZ)-containing medium formed high numbers of shoots and bud-containing tissues (BCT) were formed at the cotyledonary nodes, shoot nodes, tendrils, stipules, and internodes.
Abstract: Pea (Pisum sativum L. cv. Espace) seeds directly cultured on thidiazuron (TDZ)-containing medium formed high numbers of shoots. The number of shoots per seedling depended on the concentration and duration of the TDZ treatment. The best treatment was 12-wk incubation on MS medium supplemented with 4 mg/l TDZ followed by 4-wk culture on MS medium supplemented with 0.5 mg/l benzylaminopurine (BA) and produced more than 400 shoots/seedling. Isolated shoots rooted at a high frequency on MS medium containing 2–3 mg/l indole-3-butyric acid and 2 mg/l α-naphthalene acetic acid. In addition to the formation of shoots, bud-containing tissues (BCT) were formed at the cotyledonary nodes, shoot nodes, tendrils, stipules, and internodes. The BCT from the cotyledonary nodes and the shoot nodes was maintained in its pure state on MS medium supplemented with 4 mg/l TDZ by repeated culture. Shoot development was accomplished when the BCT were left on MS medium supplemented with 4 mg/l TDZ without subculture prior to transfer onto MS medium supplemented with 0.5 mg/l BA.

34 citations


Cites background from "Induction of morphogenesis in meris..."

  • ...In pea, protocols for somatic embryogenesis (Griga 1988, 2002; Loiseau et al. 1998) and organogenesis (Kartha et al. 1974; Kallak and Koiveer 1990) have been described; however, none of them were successful for the routine production of genetically modified plants....

    [...]

  • ...1998) and organogenesis (Kartha et al. 1974; Kallak and Koiveer 1990) have been described; however, none of them were successful for the routine production of genetically modified plants....

    [...]

Journal ArticleDOI
TL;DR: Ten genotypes from Pisum sativum and Pisum arvense were screened for their regeneration abilities and all screened genotypes were able to regenerate plants with a high efficiency although some differences in their organogenetic response were observed.
Abstract: Ten genotypes from Pisum sativum and Pisum arvense were screened for their regeneration abilities. Most of them were created through experimental mutagenesis from Bulgarian varieties and have various valuable agronomic traits. Embryonic axes from immature embryos were plated on modified Murashige and Skoog medium, containing different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-d), α-naphthaleneacetic acid (NAA) and benzyladenine (BA). Two schemes for direct and indirect organogenesis were established. Callus and shoot formation were induced on media containing 0.2 mM 2,4-d or 5 mM BA, respectively. Embryonic axes formed buds directly when plated on medium with 10 mM BA and 1 mM NAA. Organogenesis and adventitious bud formation were maintained on medium supplemented with BA and NAA. Rhizogenesis was induced on Gamborgs' B5 medium. All screened genotypes were able to regenerate plants with a high efficiency (50–100%) although some differences in their organogenetic response were observed.

17 citations


Cites result from "Induction of morphogenesis in meris..."

  • ...However, morpogenetic potential was observed (contrary to the observations of Tetu et al., 1990 and Stejskal and Griga, 1992) in all tested genotypes without morphological abnormalities ( reported by Kallak and Koiveer, 1990)....

    [...]

Journal ArticleDOI
TL;DR: A conditioning pretreatment with benzyladenine during imbibition of seed as well as during seed germination was shown to be an essential factor for flower induction and flowers originated in most cases directly from the explant and rarely arose from small shoots.

17 citations

References
More filters
Journal ArticleDOI
TL;DR: Callus formation and shoot production occurred within 4 to 6 weeks in defined media containing 0.2 to 5.0 μM benzyladenine and 1 μM naphthaleneacetic acid while most callus produced one or more shoots at high frequency, root formation did not occur regularly.
Abstract: Shoot formation was observed in callus from apical cells of pea (Pisum sativum L. cv. Century). Shoot apices from 4-day-old plants were macerated and the resulting cell masses grown on agar media. The callus formation and shoot production occurred within 4 to 6 weeks in defined media containing 0.2 to 5.0 μM benzyladenine and 1 μM naphthaleneacetic acid. While most callus produced one or more shoots at high frequency, root formation did not occur regularly. Plants obtained by these procedures were grown to maturity producing flowers and pods.

114 citations

Journal ArticleDOI
TL;DR: Whole plant regeneration via somatic embryogenesis was obtained in pea using explants from immature embryos or shoot apex segments and plantlets obtained from both zygotic embryos and shoot apices were transferred to soil and were grown to maturity.
Abstract: Whole plant regeneration via somatic embryogenesis was obtained in pea (Pisum sativum L.) using explants from immature embryos or shoot apex segments. The induction of somatic embryos required picloram or 2,4-D. Germination of fully-developed embryos was accomplished by subculture on medium with only cytokinin and then on medium supplemented with cytokinins in combination with a reduced auxin concentration. Plantlets obtained from both zygotic embryos and shoot apices were transferred to soil and were grown to maturity. Nine plants were examined cytologically, revealing three tetraploids (2n=4x=28) and six diploids (2n=2x=14).

86 citations

Journal ArticleDOI
01 Jan 1979-Planta
TL;DR: Six lines of peas were found that would regenerate after 2 months' growth as callus, but the cultivars “Frosty” and “Alaska” were among the lines that would not regenerate at all.
Abstract: Sixteen genetic lines of peas were screened for their ability to regenerate whole plants from callus cultures. Epicotyl sections from germinating seeds were placed on callus-inducing medium; the resulting callus was subcultured monthly and was tested every other month for its regeneration ability. Six lines were found that would regenerate after 2 months' growth as callus. Four of these continued to regenerate after 4 months and, of these, two after 6 months. The cultivars “Frosty” and “Alaska” were among the lines that would not regenerate at all.

80 citations

Journal ArticleDOI
TL;DR: Analysis of four isozymes, esterase, GDH, 6-PGD, and LAP, as well as nine genetically defined morphological characters indicated retention of genetic stability in the progeny of tisssue culture propagules.

67 citations

Journal ArticleDOI
TL;DR: Root formation, on the shoots produced by culturing meristems, was induced by reculturing the shoots, 2 cm long, on half strength B5 medium supplemented with NAA at a concentration of 10 −6 M.

59 citations