Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors
Kazutoshi Takahashi,Koji Tanabe,Mari Ohnuki,Megumi Narita,Tomoko Ichisaka,Kiichiro Tomoda,Shinya Yamanaka +6 more
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It is demonstrated that iPS cells can be generated from adult human fibroblasts with the same four factors: Oct3/4, Sox2, Klf4, and c-Myc.About:
This article is published in Cell.The article was published on 2007-11-30 and is currently open access. It has received 18175 citations till now. The article focuses on the topics: Induced pluripotent stem cell & Embryonic stem cell.read more
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Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro
Jason R. Spence,Christopher N. Mayhew,Scott A. Rankin,Matthew F. Kuhar,Jefferson E. Vallance,Kathryn Tolle,Elizabeth E. Hoskins,Vladimir V. Kalinichenko,Susanne I. Wells,Aaron M. Zorn,Noah F. Shroyer,James M. Wells +11 more
TL;DR: A robust and efficient process is established to direct the differentiation of human PSCs into intestinal tissue in vitro using a temporal series of growth factor manipulations to mimic embryonic intestinal development and indicates that human intestinal stem cells form de novo during development.
Journal ArticleDOI
A conditional knockout resource for the genome-wide study of mouse gene function.
William C. Skarnes,Barry Rosen,Anthony P. West,Manousos Koutsourakis,Wendy Bushell,Vivek Iyer,Alejandro O. Mujica,Alejandro O. Mujica,Mark G. Thomas,Jennifer Harrow,Tony Cox,David A. Jackson,Jessica Severin,Jessica Severin,Patrick J. Biggs,Patrick J. Biggs,Jun Fu,Michael Nefedov,Pieter J. de Jong,A. Francis Stewart,Allan Bradley +20 more
TL;DR: High-throughput genome engineering highlighted by this study is broadly applicable to rat and human stem cells and provides a foundation for future genome-wide efforts aimed at deciphering the function of all genes encoded by the mammalian genome.
Journal ArticleDOI
Hotspots of aberrant epigenomic reprogramming in human induced pluripotent stem cells
Ryan Lister,Mattia Pelizzola,Yasuyuki S. Kida,R. David Hawkins,Joseph R. Nery,Gary C. Hon,Jessica Antosiewicz-Bourget,Jessica Antosiewicz-Bourget,Ronan C. O'Malley,Rosa Castanon,Sarit Klugman,Michael Downes,Ruth T. Yu,Ron Stewart,Ron Stewart,Bing Ren,Bing Ren,James A. Thomson,Ronald M. Evans,Joseph R. Ecker +19 more
TL;DR: The first whole-genome profiles of DNA methylation at single-base resolution in five human iPSC lines, along with methylomes of ES cells, somatic cells, and differentiated iPSCs and ES cells are reported.
Journal ArticleDOI
Induced pluripotent stem cells from a spinal muscular atrophy patient
Allison D. Ebert,Junying Yu,Ferrill F. Rose,Virginia B. Mattis,Christian L. Lorson,James A. Thomson,Clive N. Svendsen +6 more
TL;DR: This is the first study to show that human induced pluripotent stem cells can be used to model the specific pathology seen in a genetically inherited disease and represents a promising resource to study disease mechanisms, screen new drug compounds and develop new therapies.
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Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes.
Trond Aasen,Angel Raya,Maria J. Barrero,Elena Garreta,Antonella Consiglio,Federico Gonzalez,Rita Vassena,Josipa Bilic,Vladimir Pekarik,Gustavo Tiscornia,Michael J. Edel,Stéphanie Boué,Juan Carlos Izpisua Belmonte +12 more
TL;DR: It is shown that reprograming of juvenile human primary keratinocytes by retroviral transduction with OCT4, SOX2, KLF4 and c-MYC is at least 100-fold more efficient and twofold faster compared with reprogramming of human fibroblasts.
References
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Journal ArticleDOI
Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
TL;DR: Induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions is demonstrated and iPS cells, designated iPS, exhibit the morphology and growth properties of ES cells and express ES cell marker genes.
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Embryonic Stem Cell Lines Derived from Human Blastocysts
James A. Thomson,Joseph Itskovitz-Eldor,Sander S. Shapiro,Michelle A. Waknitz,Swiergiel Jennifer J,Vivienne S. Marshall,Jeffrey M. Jones +6 more
TL;DR: Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primate embryonic stem cells but do not characterize other early lineages.
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Establishment in culture of pluripotential cells from mouse embryos
TL;DR: The establishment in tissue culture of pluripotent cell lines which have been isolated directly from in vitro cultures of mouse blastocysts are reported, able to differentiate either in vitro or after innoculation into a mouse as a tumour in vivo.
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Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells
TL;DR: In this article, the authors described the establishment directly from normal preimplantation mouse embryos of a cell line that forms teratocarcinomas when injected into mice and demonstrated the pluripotency of these embryonic stem cells by the observation that subclonal cultures, derived from isolated single cells, can differentiate into a wide variety of cell types.
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Core transcriptional regulatory circuitry in human embryonic stem cells.
Laurie A. Boyer,Tong Ihn Lee,Megan F. Cole,Sarah E. Johnstone,Stuart S. Levine,Jacob P. Zucker,Matthew G. Guenther,Roshan M. Kumar,Heather L. Murray,Richard G. Jenner,David K. Gifford,David K. Gifford,David K. Gifford,Douglas A. Melton,Douglas A. Melton,Rudolf Jaenisch,Richard A. Young,Richard A. Young +17 more
TL;DR: Insight is provided into the transcriptional regulation of stem cells and how OCT4, SOX2, and NANOG contribute to pluripotency and self-renewal and how they collaborate to form regulatory circuitry consisting of autoregulatory and feedforward loops.