Induction of the Lactose Transport System in a Lipid-Synthesis-Defective Mutant of Escherichia coli
Chuen Chin Hsu,C. Fred Fox +1 more
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The blocking of lipid synthesis by glycerol starvation causes a drastic decrease in inducibility of beta-galactoside transport activity relative to beta-Galactosidase, indicating that induction of lactose transport requires de novo lipid synthesis.Abstract:
In order to relate the biogenesis of the lactose transport system to lipid synthesis, a glycerol-requiring mutant of Escherichia coli K-12 with a specific defect in l-glycerol-3-phosphate synthesis was isolated and characterized. The defective enzyme is the biosynthetic l-glycerol-3-phosphate dehydrogenase [l-glycerol-3-phosphate: NAD (P) oxidoreductase, EC 1.1.1.8] which functions as a dihydroxyacetone phosphate reductase to provide l-glycerol-3-phosphate for lipid synthesis. In this mutant, removal of glycerol from the growth medium results in inhibition of the synthesis of protein, deoxyribonucleic acid, and phospholipid. Inhibition of phospholipid synthesis immediately follows glycerol removal, whereas the inhibition of deoxyribonucleic acid and protein synthesis is preceded by a short lag period. Glycerol starvation does not change the turnover pattern of previously synthesized phospholipids. The blocking of lipid synthesis by glycerol starvation causes a drastic decrease in inducibility of beta-galactoside transport activity relative to beta-galactosidase, indicating that induction of lactose transport requires de novo lipid synthesis.read more
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Physical properties of membrane lipids: biological relevance and regulation.
John E. Cronan,Edward P. Gelmann +1 more
TL;DR: This paper presents a meta-analyses of E. coli Membrane Lipids and its role in the regulation of temperature-induced alterations in fatty acids, and the role of membrane liquidity in this process.
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Enzymology, genetics, and regulation of membrane phospholipid synthesis in Escherichia coli.
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Metabolism and function of the membrane phospholipids of Escherichia coli.
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Biogenesis of microbial transport systems: Evidence for coupled incorporation of newly synthesized lipids and proteins into membrane
Golder Wilson,C. Fred Fox +1 more
TL;DR: The study of the biogenesis of the independent β-galactoside and β-glueoside transport systems of Escherichia coli K12 has been studied using an unsaturated fatty acid auxotroph, indicating a preferential association of newly synthesized transport proteins with Newly synthesized lipids.
References
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The Role of Energy Coupling in the Transport of β-Galactosides by Escherichia coli
Hans Winkler,T H Wilson +1 more
TL;DR: Evidence was consistent with the hypothesis that the same membrane carriers were involved in active transport by control cells and facilitated diffusion by poisoned cells, and the most striking finding was that the addition of metabolic inhibitors reduced the KT of exit about two orders of magnitude, whereas the Kt of entrance remained constant.
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