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Journal ArticleDOI

Industrial-scale manufacturing of pharmaceutical-grade bioactive peptides.

01 May 2011-Biotechnology Advances (Elsevier)-Vol. 29, Iss: 3, pp 272-277
TL;DR: The plausibility of manufacturing pharmaceutical-grade bioactive peptides from food proteins; the challenges and some implementable strategies for overcoming those challenges are discussed.
About: This article is published in Biotechnology Advances.The article was published on 2011-05-01. It has received 287 citations till now.
Citations
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Journal ArticleDOI
TL;DR: The overlap of technical expertise in enzyme immobilization, protein and process engineering will define the next generation of immobilized biocatalysts and the successful scale-up of their induced processes.
Abstract: Enzymes as industrial biocatalysts offer numerous advantages over traditional chemical processes with respect to sustainability and process efficiency. Enzyme catalysis has been scaled up for commercial processes in the pharmaceutical, food and beverage industries, although further enhancements in stability and biocatalyst functionality are required for optimal biocatalytic processes in the energy sector for biofuel production and in natural gas conversion. The technical barriers associated with the implementation of immobilized enzymes suggest that a multidisciplinary approach is necessary for the development of immobilized biocatalysts applicable in such industrial-scale processes. Specifically, the overlap of technical expertise in enzyme immobilization, protein and process engineering will define the next generation of immobilized biocatalysts and the successful scale-up of their induced processes. This review discusses how biocatalysis has been successfully deployed, how enzyme immobilization can improve industrial processes, as well as focuses on the analysis tools critical for the multi-scale implementation of enzyme immobilization for increased product yield at maximum market profitability and minimum logistical burden on the environment and user.

470 citations

Journal ArticleDOI
01 Jan 2011

410 citations

Journal ArticleDOI
TL;DR: In this present review, some aspects of the processes that involve protein hydrolysis steps are discussed, especially considering the application of specific proteases as a tool on food biotechnology.
Abstract: This review intended to give a brief idea of the importance of proteases applications. Processes that involve protein hydrolysis steps find wide ranging utilizations, such as cleaning process, proteomic studies, or food biotechnology process. Many positive effects hoped for with food processing can be achieved by protein hydrolysis using specific proteases, changing nutritional, bioactive and functional properties of food proteins, which include improved digestibility, modifications of sensory quality (such as texture or taste), improvement of antioxidant capability or reduction in allergenic compounds. Protease applications in industrial processes are constantly being introduced and can be advantageous compared to chemical processes, by increasing hydrolysis specificity, product preservation and purity, and reducing environmental impact. Differences in specificity between proteases are very important to take in to consideration as a guide for the choice of protease according to the protein source to be hydrolyzed or predicted products. In this present review, some aspects of the processes that involve protein hydrolysis steps are discussed, especially considering the application of specific proteases as a tool on food biotechnology.

397 citations

Journal ArticleDOI
01 Apr 2014-Peptides
TL;DR: This review focuses on bioactive peptides derived from soybean and illustrates their production and biofunctional attributes.

383 citations

References
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Journal ArticleDOI
TL;DR: The role of cationic host-defense peptides in modulating the innate immune response and boosting infection-resolving immunity while dampening potentially harmful pro-inflammatory (septic) responses gives these peptides the potential to become an entirely new therapeutic approach against bacterial infections.
Abstract: Short cationic amphiphilic peptides with antimicrobial and/or immunomodulatory activities are present in virtually every life form, as an important component of (innate) immune defenses. These host-defense peptides provide a template for two separate classes of antimicrobial drugs. Direct-acting antimicrobial host-defense peptides can be rapid-acting and potent, and possess an unusually broad spectrum of activity; consequently, they have prospects as new antibiotics, although clinical trials to date have shown efficacy only as topical agents. But for these compounds to fulfill their therapeutic promise and overcome clinical setbacks, further work is needed to understand their mechanisms of action and reduce the potential for unwanted toxicity, to make them more resistant to protease degradation and improve serum half-life, as well as to devise means of manufacturing them on a large scale in a consistent and cost-effective manner. In contrast, the role of cationic host-defense peptides in modulating the innate immune response and boosting infection-resolving immunity while dampening potentially harmful pro-inflammatory (septic) responses gives these peptides the potential to become an entirely new therapeutic approach against bacterial infections.

3,556 citations

Journal ArticleDOI
TL;DR: A variety of naturally formed bioactive peptides have been found in fermented dairy products, such as yoghurt, sour milk and cheese as mentioned in this paper, but the health benefits attributed to peptides in these traditional products have, so far, not been established, however.

1,465 citations


"Industrial-scale manufacturing of p..." refers background in this paper

  • ...Several studies in the past few decades have established that bioactive peptides have certain bio-functionalities and may therefore serve therapeutic roles in body systems (Hancock and Sahl, 2006; Huang et al., 2010; Kim andWijesekara, 2010; Korhonen and Pihlanto, 2006)....

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  • ...Second, the proteases of most microorganisms, especially the Lactic Acid Bacteria are expressed on the cell-membrane making harvesting and purification relatively cheap and less laborious....

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  • ...…is more efficient under industrial-scale conditions and less costive than the convectional batch methods and it is achieved by the use of compartmentalized ultrafiltration membrane reactors which may or may not be coupled with other purification techniques/units (Korhonen and Pihlanto, 2006)....

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  • ...Another common approach is the use of continuous processes which has been widely applied for total conversion of food proteins of various origins to hydrolysates with improved nutritional and/or functional properties (Korhonen and Pihlanto, 2006)....

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  • ...The unavailability of large scale technologies and high cost of purification techniques are a limiting factor to the commercialization of peptide-based products (Korhonen and Pihlanto, 2006)....

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Journal ArticleDOI
TL;DR: Several products are already on the market or under development by food companies that exploit the potential of food-derived bioactive peptides and which ascribe scientifically evidenced health claims to consumption of these functional foods.

986 citations

Journal ArticleDOI
TL;DR: An overview of the bioactive peptides derived from marine organisms and their biological activities with potential applications in different areas is presented.

652 citations

Journal ArticleDOI
TL;DR: There is evidence for the antihypertensive effects of milk products fermented with Lactobacillus helveticus containing the tripeptides IPP and VPP, which inhibit angiotensin converting enzyme, and for osteoprotective effects by milk basic protein.
Abstract: Some dietary proteins cause specific effects going beyond nutrient supply. A number of proteins seem to act directly in the intestine, such as IGFs, lactoferrin and immunoglobulins. Many substances, however, are peptides encrypted in intact molecules and are released from their encrypted position by enzymes during gastrointestinal transit or by fermentation or ripening during food processing. Among food-derived bioactive proteins and peptides from plants and animals, those obtained from milk are known in particular. Numerous effects have been described after in vitro and animal trials for bioactive proteins and peptides, such as immunomodulating, antihypertensive, osteoprotective, antilipemic, opiate, antioxidative and antimicrobial. This article reviews the current knowledge of the existence of bioactive proteins and of in vitro bioactivity and the present evidence of health effects exerted by such substances or products containing bioactive compounds. For example, there is evidence for the antihypertensive effects of milk products fermented with Lactobacillus helveticus containing the tripeptides IPP and VPP, which inhibit angiotensin converting enzyme, and for osteoprotective effects by milk basic protein. There is less profound evidence on the immunomodulating effects of lactoferrin and postprandial triglyceride reduction by a hydrolysate of bovine hemoglobin.

519 citations


"Industrial-scale manufacturing of p..." refers background in this paper

  • ...Bioactive peptides are protein hydrolysates that can induce beneficial physiological responses in the human body (Möller et al., 2008)....

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  • ...The functional abilities of bioactive peptides have therefore aroused a lot of scientific, technological and consumer interest as regards the role of dietary proteins in controlling and influencing health (Möller et al., 2008)....

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