Journal ArticleDOI
Infections with weakly haemolytic Brachyspira species in pigs with miscellaneous chronic diseases
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This investigation shows that infections with weakly haemolytic Brachyspira spp.About:
This article is published in Veterinary Microbiology.The article was published on 2009-03-02. It has received 39 citations till now. The article focuses on the topics: Brachyspira.read more
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Journal ArticleDOI
Reproduction of Mucohaemorrhagic Diarrhea and Colitis Indistinguishable from Swine Dysentery following Experimental Inoculation with “Brachyspira hampsonii” Strain 30446
Joseph E. Rubin,Matheus O. Costa,Janet E. Hill,Heather E. Kittrell,Champika Fernando,Yanyun Huang,Brendan O'Connor,John C. S. Harding +7 more
TL;DR: “Brachyspira hampsonii” strain 30446 causes mucohaemorrhagic diarrhea in pigs following a 4–9 day incubation period, and culture and 30446-specific qPCR are reliable methods of detection of this organism in feces and tissues of diarrheic pigs.
Journal ArticleDOI
Comparative virulence of clinical Brachyspira spp. isolates in inoculated pigs
Eric R. Burrough,Erin L. Strait,Joann M. Kinyon,Leslie Bower,Darin M. Madson,B. L. Wilberts,Kent Schwartz,Timothy S. Frana,J. Glenn Songer +8 more
TL;DR: Results suggest that phenotypic culture characteristics of Brachyspira spp.
Journal ArticleDOI
The use of quantitative PCR for identification and quantification of Brachyspira pilosicoli, Lawsonia intracellularis and Escherichia coli fimbrial types F4 and F18 in pig feces.
Marie Ståhl,Branko Kokotovic,Charlotte Kristiane Hjulsager,Solvej Østergaard Breum,Øystein Angen +4 more
TL;DR: The qPCR assay for Lawsonia was found to be more sensitive than cultivation for E. coli and B. pilosicoli and the parallel simultaneous analysis for several bacteria in multi-qPCR and the determination of the quantities of the infectious agents increases the information obtained from the samples and the chance for obtaining a relevant diagnosis.
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Complete genome sequence of Brachyspira intermedia reveals unique genomic features in Brachyspira species and phage-mediated horizontal gene transfer
TL;DR: Evidence that bacteriophages were being remodeled and genes incorporated into them within the Brachyspira genomes is found, and a mechanism for horizontal gene transfer appears to be gene translocations leading to remodeling of bacter iophages in combination with broad tropism.
Journal ArticleDOI
Characterization and recognition of Brachyspira hampsonii sp. Nov., a novel intestinal spirochete that is pathogenic to pigs
TL;DR: B. hampsonii is classified as a unique species with genetically diverse yet phenotypically similar genomovars (I, II, and III) and the type strain NSH-16 (= ATCC BAA-2463 = NCTC 13792).
References
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Book
Clinical veterinary microbiology
TL;DR: General techniques laboratory safety collection and submission of diagnostic specimens essential equipment and reagents for a veterinary diagnostic microbiology laboratory diagnostic applications of immunological tests
Journal ArticleDOI
Pathological, Immunohistochemical, and In-situ Hybridization Studies of Natural Cases of Postweaning Multisystemic Wasting Syndrome (PMWS) in Pigs
C. Rosell,Joaquim Segalés,J. Plana-Durán,Monica Balasch,G. M. Rodríguez-Arrioja,S. Kennedy,Gordon Allan,Francis McNeilly,K. S. Latimer,Mariano Domingo +9 more
TL;DR: Fifteen pigs from five farms on which there had been a previous clinical and histopathological diagnosis of postweaning multisystemic wasting syndrome (PMWS) were investigated, and lymphoid lesions were suggestive of immunosuppression.
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Amplification of the ZFY and ZFX genes for sex identification in humans, cattle, sheep and goats.
Eric Aasen,Juan F. Medrano +1 more
TL;DR: In this paper, the authors used universal primers to amplify 447 or 445 bp fragments from male or female genomic DNA corresponding to the ZFY or ZFX genes and found specific banding patterns between the two sexes in these species.
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Autoclaved liquid medium for propagation of Treponema hyodysenteriae.
TL;DR: Three liquid media that differ slightly in composition but not in the method of preparation were developed for the propagation of TrePonema hyodysenteriae and Treponema innocens and supported better growth of T. hyodysteriae than did previously used liquid media.
Journal ArticleDOI
Selective medium for the isolation of Treponema hyodysenteriae.
SR Jenkinson,CR Wingar +1 more