Inflammation and pain in skin and deep tissues

doi:10.5278/VBN.PHD.MED.00015

Home
/
Papers
/
Inflammation and pain in skin and deep tissues

Inflammation and pain in skin and deep tissues

01 Jan 2015-

TL;DR: Evaluate the change in sensitivity after inflammation inside and outside the irradiated skin and investigate the existence of a mechanism of cumulative effects between cutaneous and deep-tissue hyperalgesia and allodynia induced by UVB irradiation after application of heat stimuli and sensitization of deep tissues.

read less

Abstract: Ultraviolet-B irradiation model has been used for many years as a translational model since it is well known to induce cutaneous inflammatory pain in both animals and humans. The aim of this study project was to evaluate time course and sensory changes induced by UVB and in particular: 1) evaluate the change in sensitivity after inflammation inside and outside the irradiated skin, 2) investigate the existence of a mechanism of cumulative effects between cutaneous and deep-tissue hyperalgesia, 3) investigate the effect on mechanical hyperalgesia and allodynia induced by UVB irradiation after application of heat stimuli and sensitization of deep tissues.

...read moreread less

Content maybe subject to copyright Report

Citations

PDF

Open Access

More filters

Book Chapter•

Wall and Melzack's textbook of pain

[...]

Joanna M Zakrzewska, Benjamin C. Lopez

01 Jan 2006

TL;DR: The Wall and Melzack's Textbook of Pain is revised under new editorial leadership, and with a host of new, multidisciplinary international contributors.

...read moreread less

Abstract: WALL AND MELZACK'S TEXTBOOK OF PAIN, revised under new editorial leadership, and with a host of new, multidisciplinary international contributors ...

...read moreread less

527 citations

Journal Article•DOI•

Increased content and transport of substance P and calcitonin gene-related peptide in sensory nerves innervating inflamed tissue: Evidence for a regulatory function of nerve growth factor in vivo

[...]

Josef Donnerer, Rufina Schuligoi, Rainer Amann, Christoph Stein

01 May 1992-Neuropeptides

TL;DR: Findings point towards a regulatory function for nerve growth factor in vivo in the stimulation of sensory neuropeptide synthesis during prolonged inflammatory processes.

...read moreread less

223 citations

Journal Article•DOI•

Core Topics in Pain.

[...]

A Philip J. Lake

01 Apr 2007-Anesthesiology

19 citations

Journal Article•DOI•

Sensory defunctionalization induced by 8% topical capsaicin treatment in a model of ultraviolet-B-induced cutaneous hyperalgesia.

[...]

Silvia Lo Vecchio¹, Hjalte Holm Andersen¹, Jesper Elberling², Lars Arendt-Nielsen¹•Institutions (2)

Aalborg University¹, Copenhagen University Hospital²

24 Jul 2021-Experimental Brain Research

TL;DR: In this article, the authors explored the contribution of capsaicin-sensitive nociceptors in the development of mechanical and heat hyperalgesia in humans following ultraviolet-B (UVB) irradiation.

...read moreread less

Abstract: Subpopulations of primary nociceptors (C- and Aδ-fibers), express the TRPV1 receptor for heat and capsaicin. During cutaneous inflammation, these afferents may become sensitized, leading to primary hyperalgesia. It is known that TRPV1+ nociceptors are involved in heat hyperalgesia; however, their involvement in mechanical hyperalgesia is unclear. This study explored the contribution of capsaicin-sensitive nociceptors in the development of mechanical and heat hyperalgesia in humans following ultraviolet-B (UVB) irradiation. Skin areas in 18 healthy volunteers were randomized to treatment with 8% capsaicin/vehicle patches for 24 h. After patches removal, one capsaicin-treated area and one vehicle area were irradiated with 2xMED (minimal erythema dose) of UVB. 1, 3 and 7 days post-UVB exposure, tests were performed to evaluate the development of UVB-induced cutaneous hyperalgesia: thermal detection and pain thresholds, pain sensitivity to supra-threshold heat stimuli, mechanical pain threshold and sensitivity, touch pleasantness, trans-epidermal water loss (TEWL), inflammatory response, pigmentation and micro-vascular reactivity. Capsaicin pre-treatment, in the UVB-irradiated area (Capsaicin + UVB area), increased heat pain thresholds (P 0.2). No effects of capsaicin were reported on touch pleasantness (P = 1), TEWL (P = 0.31), inflammatory response and pigmentation (P > 0.3) or micro-vascular reactivity (P > 0.8) in response to the UVB irradiation. 8% capsaicin ablation predominantly defunctionalizes TRPV1+-expressing cutaneous nociceptors responsible for heat pain transduction, suggesting that sensitization of these fibers is required for development of heat hyperalgesia following cutaneous UVB-induced inflammation but they are likely only partially necessary for the establishment of robust primary mechanical hyperalgesia.

...read moreread less

1 citations

References

PDF

Open Access

More filters

Journal Article•DOI•

Ultraviolet-B-Induced Erythema is Mediated by Nitric Oxide and Prostaglandin E2 in Combination

[...]

Lesley E. Rhodes, G. Belgi¹, Richard Parslew², L. McLoughlin², Geraldine F. Clough¹, Peter S. Friedmann¹ - Show less +2 more•Institutions (2)

University of Southampton¹, Royal Liverpool University Hospital²

01 Oct 2001-Journal of Investigative Dermatology

TL;DR: Findings confirm prostaglandin E2 and NO to be mediators of ultraviolet-induced erythema and show that there is prolonged synthesis of both mediators within the ery themal response and that synthesis of NO is induced by lower doses of ultraviolet B compared with that of prostaglanders E2.

...read moreread less

115 citations

"Inflammation and pain in skin and d..." refers background in this paper

...In fact, Rhodes and collaborators used microdialysis t echnique in the UVB irradiated skin and confirmed that NO is one of the most important infl ammatory mediators released that can directly be involved in the skin response to UVB irradiation (Clough and Church 2002; Rhodes et al., 2001)....
[...]
...In fact, Rhodes and collaborators used microdialysis technique in the UVB irradiated skin and confirmed that NO is one of the most important inflammatory mediators released that can directly be involved in the skin response to UVB irradiation (Clough and Church 2002; Rhodes et al., 2001)....
[...]

Journal Article•DOI•

Experimental muscle pain and tenderness following infusion of endogenous substances in humans

[...]

Hanne Mørk¹, Messoud Ashina¹, Lars Bendtsen¹, Jes Olesen¹, Rigmor Jensen¹ - Show less +1 more•Institutions (1)

University of Copenhagen¹

01 Apr 2003-European Journal of Pain

TL;DR: Intramuscular infusion of a combination of Bk, 5‐HT, His, and PGE2 induced a prolonged moderate pain and tenderness in healthy humans, and this model may be a valuable tool in future studies of the pathophysiological mechanisms of myofascial pain.

...read moreread less

112 citations

"Inflammation and pain in skin and d..." refers background in this paper

...This sensitization mimics the inflammatory condition and the decrease in pain thresholds prese nt in some disorders involving the musculoskeletal system, making the models very usef ul from a clinical perspective (Mørk et al., 2003; Staahl and Drewes 2004)....
[...]
...This sensitization mimics the inflammatory condition and the decrease in pain thresholds present in some disorders involving the musculoskeletal system, making the models very useful from a clinical perspective (Mørk et al., 2003; Staahl and Drewes 2004)....
[...]

Journal Article•DOI•

Erythema and pigmentation

[...]

Herbert Hönigsmann¹•Institutions (1)

University of Vienna¹

01 Apr 2002-Photodermatology, Photoimmunology and Photomedicine

TL;DR: The acute clinical effects of exposure to sunlight or artificial UV radiation consist of erythema (sunburn) and pigmentation (tanning) as well as thickening of the epidermis, in particular the stratum corneum (‘Lichtschwiele’).

...read moreread less

Abstract: The acute clinical effects of exposure to sunlight or artificial UV radiation consist of erythema (sunburn) and pigmentation (tanning) as well as thickening of the epidermis, in particular the stratum corneum (‘Lichtschwiele’). There are also other biological reactions, such as immunosuppression and the photosynthesis of vitamin D, which will not be discussed here. Most human UV radiation exposure is to sunlight, but other sources such as phototherapy lamps, sunbeds, arc-welding apparatus and unshielded fluorescent and tungsten-halogen lamps can cause similar effects. The individual erythema and tanning responses of human skin are basically genetically determined. Studies indicate a possible role for polymorphisms in the MSH receptor (1), although other genes may also be involved.

...read moreread less

107 citations

"Inflammation and pain in skin and d..." refers background in this paper

...This reaction is generally classified as a superficial or first-degree burn (McStay ; Soter 1990), and is considered an acute inflammatory reaction caused by an excessive exposure of the epidermis to UVB radiations (Honigsmann 2002)....
[...]
...Humans are normally exposed to UVB radiation through natural sunlight but there are also artificial sources of UVB radiation such as sun-beds, phototherapy lamps or tungsten-halogen lamps (Honigsmann 2002)....
[...]
...UVB irradiation also induces increased pigmentation and thickening of the epidermis (Honigsmann 2002)....
[...]
...The UVB-irradiation is mostly absorbed by the chromophores present in the epidermis and leads to destruction of epidermal cells, like keratinocytes and to release of numerous cytokines and inflammatory mediators into the skin (Honigsmann 2002)....
[...]

Journal Article•DOI•

Algometer reliability in measuring pain pressure threshold over normal spinal muscles to allow quantification of anti-nociceptive treatment effects

[...]

Louise Potter¹, Christopher J. McCarthy², Jacqueline Oldham¹•Institutions (2)

University of Manchester¹, University of Warwick²

01 Dec 2006-International Journal of Osteopathic Medicine

TL;DR: This study provides further validity to the use of algometry as a suitable, convenient method of monitoring treatment effects and shows it is a reliable, both within-session and between-sessions, measure of a subject's pain.

...read moreread less

106 citations

"Inflammation and pain in skin and d..." refers methods in this paper

...…been used for evaluation of sensitivity to pain and the assessment of pressure perception but this methodology has a certain amount of variability, even though it is considered a very reliable method for measuring pressure pain thresholds (Fischer 1987; Potter et al., 2006; Reeves et al., 1986)....
[...]

Journal Article•DOI•

Cytokine profile in human skin in response to experimental inflammation, noxious stimulation, and administration of a COX-inhibitor: a microdialysis study.

[...]

Martin S. Angst¹, J D Clark¹, J D Clark², Brendan Carvalho¹, Martha Tingle¹, Martin Schmelz³, David C. Yeomans¹ - Show less +3 more•Institutions (3)

Stanford University¹, Veterans Health Administration², Heidelberg University³

30 Sep 2008-Pain

TL;DR: The utility of explored method for tracking cytokines in human tissue is supported and the data suggest that high clinical doses of ibuprofen exert anti‐inflammatory effects by down‐regulating tissue cytokine levels.

...read moreread less

Abstract: Animal studies have documented a critical role for cytokines in cell signaling events underlying inflammation and pain associated with tissue injury. While clinical reports indicate an important role of cytokines in inflammatory pain, methodological limitations have made systematic human studies difficult. This study examined the utility of a human in vivo bioassay combining microdialysis with multiplex immunoassay techniques for measuring cytokine arrays in tissue. The first experiment measured cytokines in interstitial fluid collected from non-inflamed and experimentally inflamed skin (UVB). The effects of noxious heat on cytokine release were also assessed. The second experiment examined whether anti-hyperalgesic effects of the COX-inhibitor ibuprofen were associated with decreased tissue levels of the pro-inflammatory cytokines IL-1 beta and IL-6. In the first experiment, inflammation significantly increased IL-1 beta, IL-6, IL-8, IL-10, G-CSF, and MIP-1 beta. Noxious heat but not experimental inflammation significantly increased IL-7 and IL-13. In the second experiment, an oral dose of 400 and 800 mg ibuprofen produced similar anti-hyperalgesic effects suggesting a ceiling effect. Tissue levels of IL-1 beta and IL-6 were not affected after the 400mg dose but decreased significantly (44+/-32% and 38+/-13%) after the 800 mg dose. These results support the utility of explored method for tracking cytokines in human tissue and suggest that anti-hyperalgesic and anti-inflammatory effects of ibuprofen are at least partially dissociated. The data further suggest that high clinical doses of ibuprofen exert anti-inflammatory effects by down-regulating tissue cytokine levels. Explored human bioassay is a promising tool for studying the pathology and pharmacology of inflammatory and chronic pain conditions.

...read moreread less

101 citations

"Inflammation and pain in skin and d..." refers background in this paper

...It is believed that UVB inflammation releases a number of vasoactive inflammatory mediators acting directly on the vasculature (Angst et al., 2008)....
[...]