Journal ArticleDOI
Influence of selenium on glutathione and some associated enzymes in rats with mammary tumor induced by 7,12-Dimethylbenz(a)anthracene
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TLDR
Observations clearly demonstrate the influence of dietary selenium supplementation in correcting abnormal changes in glutathione turnover and some associated enzymes in tumor induced rats.Abstract:
A recent finding in epidemiological and laboratory studies suggests that the ratio of selenium to glutathione is lower in breast cancer subjects than its control counterparts. Selenium, an antioxidant and anticarcinogen, can modify the status of glutathione and some associated enzymes by blocking peroxidation of lipids in membranes of cancer subjects. Studies were conducted using female albino rats of Wistar strain bearing mammary tumor induced by 7,12-dimethylbenz(a) anthracene to assess the biological role of selenium on some antioxidant enzymes associated with the maintenance of glutathione status. For induction of mammary tumor, 25 mg DMBA in a 1 ml emulsion of sunflower oil and physiological saline was injected subcutaneously to each rat. One group in each of control and tumor bearing rats, were fed 5 mg sodium selenite/kg diet from the day of tumor induction for 24 weeks. Increase in the reduced glutathione concentration was preceded by significant increase in the oxidized glutathione as well as in the activities of γ-glutamylcysteine synthetase, glutathione peroxidase, glutathione reductase, glutathione S-transferase, and glucose-6-phosphate dehydrogenase by selenium administration in rats bearing tumor. However, selenium administration to rats bearing tumor decreased the activity of γ-glutamyl transpeptidase. These observations clearly demonstrate the influence of dietary selenium supplementation in correcting abnormal changes in glutathione turnover and some associated enzymes in tumor induced rats.read more
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Effect of allyl isothiocyanate on NF-κB signaling in 7,12-dimethylbenz(a)anthracene and N-methyl-N-nitrosourea-induced mammary carcinogenesis.
Thangarasu Rajakumar,Pachaiappan Pugalendhi,Rajendran Jayaganesh,Dhanabalan Ananthakrishnan,K. Gunasekaran +4 more
TL;DR: The result suggests that AITC has anti-inflammatory potential to prevent DMBA- and MNU-induced mammary carcinogenesis in rats.
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Allyl isothiocyanate, a potent chemopreventive agent targets AhR/Nrf2 signaling pathway in chemically induced mammary carcinogenesis
Thangarasu Rajakumar,Pachaiappan Pugalendhi,Subbaiyan Thilagavathi,Dhanabalan Ananthakrishnan,K. Gunasekaran +4 more
TL;DR: AITC prevents DMBA-induced mammary carcinogenesis via inhibition of phase I and induction of phase II detoxification enzymes by modulating AhR/Nrf2 signaling pathway and histopathological results confirmed that AITC protectsDMBA-mediated hepatocellular damage.
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Inhibitory effect of selenium on N-nitrosodiethylamine and phenobarbital-promoted rat liver carcinogenesis
TL;DR: Selenium treatment in-creased the activity of this enzyme in kidney, lung, and erythrocytes to a significant level compared with the tissue or cell levels of the carcinogen control rats and confirmed the fact that selenium is particularly protective in limiting the action of DEN during the initiation phase of hepatocarcinogenesis.
Journal ArticleDOI
Dose‐dependent chemopreventive effects of citronellol in DMBA‐induced breast cancer among rats
TL;DR: The result suggests that the citronellol of 50 mg/kg body weight exerted significant chemopreventive effects and can be considered as a minimum optimum dose in the prevention of mammary carcinogenesis.
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Antitumoral effects of Allium sivasicum on breast cancer in vitro and in vivo.
Bektas Tepe,Ersin Tuncer,Serpil Ünver Saraydin,Hatice Özer,Metin Şen,Kursat Karadayi,Deniz Sahin Inan,Sule Karadayi,Zubeyde Akin Polat,Aşkın Akpulat,Mustafa Duman,Binnur Koksal,Mustafa Turan +12 more
TL;DR: Results indicate that A. sivasicum has antitumoral potential against breast cancer.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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Selenium: Biochemical Role as a Component of Glutathione Peroxidase
TL;DR: When hemolyzates from erythrocytes of selenium-deficient rats were incubated in vitro in the presence of ascorbate or H2O2, added glutathione failed to protect the hemoglobin from oxidative damage.
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Enzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione: Applications to mammalian blood and other tissues
TL;DR: The use of the foregoing analytical method in the determination of total and oxidized glutathione contents of rat blood, kidney, and liver gave values in good agreement with those obtained by previous investigators.