Figure 3 | Nucleolar disruption induced by an RNA polymerase I inhibitor impairs viral replication, transcription, and helical vRNP formation. a, b, f, A549 cells were pretreated with CX5461, 10 µg/mL actinomycin D (Act D), or 1% DMSO (Vehicle) for 2 h, followed by wild-type virus infection (MOI=5) for 5 h. a, Selectivity of the RNA polymerase inhibitors on Pol I and II activities. Total RNA was extracted and analysed by RT-qPCR. The expression levels were compared with that of vehicletreated cells using one-way ANOVA with Dunnett’s test; ***P<0.001. Data are presented as mean ± S.D. of three independent experiments. b, CX5461-induced nucleolar disruption and its effect on NP expression. Scale bars, 20 µm. Representative images from three independent experiments. c, Immunoprecipitation of vRNPs from the PB2-FLAG virus-infected A549 cells (MOI=5), followed by 10 µM CX5461 or
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