Figure 4b, and Supplementary movie 2). In contrast, NPNoLSmut were barely assembled 161 into double-helical structures and the resultant vRNPs showed pleomorphic morphology 162 with a height of ≤5 nm, where string-like structures, probably naked RNAs based on 163 their structure, were exposed (Figure 2c, 2d, 2e, and Supplementary movie 3). 164 Importantly, NoLS-NPNoLSmut was also assembled into double-helical vRNPs (~65% of 165 the vRNPs) (Figure 2c, 2d, 2e, and Supplementary movie 4). Immunoelectron 166 microscopy confirmed that both double-helical vRNPs and the pleomorphic aggregates 167 comprised NP and viral polymerase (Extended Data Figure 4a), indicating that the 168 pleomorphic aggregates are abortive vRNPs. Taken together, these data demonstrate 169 that the nucleolar NP localization is critical for functional double-helical vRNP 170 formation. 171
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