Inhibition of Cdc42 during mitotic exit is required for cytokinesis
Benjamin D. Atkins,Satoshi Yoshida,Satoshi Yoshida,Koji Saito,Chi-Fang Wu,Daniel J. Lew,David Pellman,David Pellman +7 more
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TLDR
A decrease in Cdc42 activation during mitotic exit is necessary to allow localization of key cytokinesis regulators and proper septum formation.Abstract:
The role of Cdc42 and its regulation during cytokinesis is not well understood. Using biochemical and imaging approaches in budding yeast, we demonstrate that Cdc42 activation peaks during the G1/S transition and during anaphase but drops during mitotic exit and cytokinesis. Cdc5/Polo kinase is an important upstream cell cycle regulator that suppresses Cdc42 activity. Failure to down-regulate Cdc42 during mitotic exit impairs the normal localization of key cytokinesis regulators-Iqg1 and Inn1-at the division site, and results in an abnormal septum. The effects of Cdc42 hyperactivation are largely mediated by the Cdc42 effector p21-activated kinase Ste20. Inhibition of Cdc42 and related Rho guanosine triphosphatases may be a general feature of cytokinesis in eukaryotes.read more
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Journal ArticleDOI
Cell Polarity in Yeast.
TL;DR: Findings from budding and fission yeasts that reveal both a conserved core polarity circuit and several adaptations that each organism exploits to fulfill the needs of its lifestyle are reviewed.
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Distinct roles of Rho1, Cdc42, and Cyk3 in septum formation and abscission during yeast cytokinesis
TL;DR: Rho1 inactivation by Cyk3 promotes primary septum formation and cleavage-furrow ingression, whereas Rho1 activation or Cdc42 inactivation promotes secondary septal formation and abscission.
Journal ArticleDOI
Cell cycle entry triggers a switch between two modes of Cdc42 activation during yeast polarization
TL;DR: Using optogenetics, yeast polarization is probed and it is found that local recruitment of Cdc24 or Bem1 is sufficient to induce polarization by triggering self-sustaining Cdc42 activity, however, the response to these perturbations depends on the recruited molecule, the cell cycle stage, and existing polarization sites.
Journal ArticleDOI
Cell-cycle control of cell polarity in yeast.
TL;DR: It is reported that yeast daughter cells can polarize Cdc42 before CDK activation at start, and it is shown that G1 cyclin‐dependent kinase activity enables localization of a subset of CDC42 effectors to sites enriched for C dc42.
Journal ArticleDOI
The final cut: cell polarity meets cytokinesis at the bud neck in S. cerevisiae
TL;DR: This review recapitulates how budding yeast cells undergo polarized processes at the bud neck for cell division, and divides asymmetrically, producing two cells with distinct sizes and fates.
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