Installation of efficient quenching groups of a fluorescent probe for the specific detection of cysteine and homocysteine over glutathione in solution and imaging of living cells
Ajit Kumar Mahapatra,Srimanta Manna,Parthasarathi Karmakar,Kalipada Maiti,Rajkishor Maji,Debasish Mandal,Raihan Uddin,Sukhendu Mandal +7 more
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TLDR
In this article, the authors reported the synthesis and characterisation of a new fluorescent probe 4-(7-nitro-benzo[1,2,5]oxadiazol-4-yl)-benzaldehyde (NBOB) installed with quenching groups for highly selective and sensitive sensing of biothiols.Abstract:
Herein, we report the synthesis and characterisation of a new fluorescent probe 4-(7-nitro-benzo[1,2,5]oxadiazol-4-yl)-benzaldehyde (NBOB) installed with quenching groups for highly selective and sensitive sensing of biothiols. The probe itself is non-fluorescent due to the presence of quenching groups and photoinduced electron transfer (PET) process. Thus, sensitivity of the probe towards thiols was significantly improved by quenching effects. NBOB has been shown to exhibit selective reactivity towards cysteine (Cys) and homocysteine (Hcy) over glutathione (GSH) under stoichiometric conditions. The response mechanism was proved by 1H NMR, LCMS and theoretical calculation. The probe NBOB has been shown to react with Cys present in Vero cells by fluorescence microscopy.read more
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Recent advances in the development of responsive probes for selective detection of cysteine
TL;DR: In this review, advances in the development of Cys-responsive luminescence probes, including molecular probes and nanoprobes, are included and discussed in sections corresponding to their response mechanisms.
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A dual emission fluorescent probe enables simultaneous detection and discrimination of Cys/Hcy and GSH and its application in cell imaging
TL;DR: CA-NBD showed high selectivity towards Cys/Hcy and GSH over other analytes with apparent colorimetric and fluorescent signals and was successfully used to detect thiols in living Hela cells with low cytotoxicity.
Journal ArticleDOI
Fluorescent probes for detecting cysteine.
TL;DR: In this review, fluorescent probes that detect cysteine over the past three years are summarized based on structural features of fluorophores such as coumarin, BODIPY, rhodamine, fluorescein, CDs, QDs, etc and reaction groups including acrylate, aldehyde, halogen, 7-nitrobenzofurazan, etc.
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A coumarin-based fluorescent probe for specific detection of cysteine in the lysosome of living cells
Yayi Tu,Mojib Latif +1 more
TL;DR: In this article , a coumarin-based fluorescent probe MCA for the detection of Cys in living cells was developed, which exhibited high sensitivity (detection limit = 6.8 nM) and selectivity towards Cys superior to other analytes.
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Chemistry of Substituted Thiazinanes and Their Derivatives.
TL;DR: This review was focused on the synthetic approaches of thiazinane derivatives and their chemical reactivity.
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Leonore A. Herzenberg,Stephen C. De Rosa,J. Gregson Dubs,Mario Roederer,M. T. Anderson,Stephen W. Ela,Stanley C. Deresinski,Leonard A. Herzenberg +7 more
TL;DR: Clinical studies presented here directly demonstrate that low GSH levels predict poor survival in otherwise indistinguishable HIV-infected subjects and establishes GSH deficiency as a key determinant of survival in HIV disease.
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TL;DR: In this article, the authors show that GSH deficiency in CD4Tcells from such subjectsisassociated with markedly decreased survival 2-3 years after baseline data collection (Kaplan-Meier and logistic regression analyses, P < 0.0001 for both analyses).