Journal ArticleDOI
Interaction of chlorpromazine with myoglobin and hemoglobin: A comparative study
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TLDR
Thermodynamic analysis revealed that binding of CPZ to hemoglobin was exothermic, whereas binding to myoglobin was endothermic with a high entropic contribution, suggesting that CPZ binding toMyoglobin is hydrophobic in nature.About:
This article is published in Biochemical Pharmacology.The article was published on 1994-06-01. It has received 68 citations till now. The article focuses on the topics: Myoglobin & Binding constant.read more
Citations
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Journal ArticleDOI
Binding of [Cr(phen)3](3+) to transferrin at extracellular and endosomal pHs: potential application in photodynamic therapy.
TL;DR: It is proposed that the TfR could recognize the holoTf despite having a chromium complex associated, and the binding process would occur in a site different from the specific iron binding sites of the protein and would be the same in both protein states.
Journal ArticleDOI
Exonuclease I-assisted fluorescence aptasensor for tetrodotoxin.
TL;DR: The fluorescence aptasensor shows good analytical performance with the linear increase of fluorescence intensity at the tetrodotoxin concentration from 0.030 nM to 6.0 × 103 NM, and the detection limit of 11.0 pM is much lower than that of other reported sensor methods.
Journal ArticleDOI
A label-free Exonuclease I-assisted fluorescence aptasensor for highly selective and sensitive detection of silver ions
TL;DR: In this article, a label-free Exonuclease I-assisted fluorescence aptamer sensing platform was established for the detection of silver ions with high sensitivity and selectivity, based on the specific interaction of Ag+ and cytosine-cytosine (C-C) base mismatch.
Journal ArticleDOI
Energetics of binding and protein unfolding upon amphiphilic drug complexation with a globular protein in different aqueous media.
Mohammad Arif Cheema,Pablo Taboada,Silvia Barbosa,Manuel Gutiérrez-Pichel,Manuel Gutiérrez-Pichel,Emilio Castro,Mohammad Siddiq,Víctor Mosquera +7 more
TL;DR: The interactions and complexation process of the structurally related amphiphilic phenothiazines promazine and triflupromazine hydrochlorides with horse myoglobin in aqueous buffered solutions of pH 2.5, 5.5 and 9.0 is examined to think this is a consequence of the already expanded structure of the protein at this pH and the subsequent binding of drug molecules to the protein.
Journal ArticleDOI
Methyglyoxal administration induces modification of hemoglobin in experimental rats: An in vivo study.
TL;DR: The modifications of Arg-31α, Arg-92α and Arg-104β of methylglyoxal-treated rat hemoglobin appear to be associated with the observed structural alterations of the heme protein, and may have physiological significance.
References
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Journal ArticleDOI
Myoglobin-facilitated oxygen diffusion: role of myoglobin in oxygen entry into muscle.
Journal ArticleDOI
Evidence for cooperative binding of chlorpromazine with hemoglobin: equilibrium dialysis, fluorescence quenching and oxygen release study.
TL;DR: Binding of chlorpromazine with human hemoglobin has been studied by equilibrium dialysis and fluorescence quenching and results revealed that the binding was positively cooperative with overall affinity constant K = 3.8 x 10(3) M-1.
Book ChapterDOI
Preparation of myoglobins.
TL;DR: This chapter presents procedures for the isolation of intracellular oxygen-binding proteins of tissues, called “tissue hemoglobins” in the widest sense, which are monomers or dimers having a minimum molecular weight of 18,000 with similar optical spectra and chemical reactivity.
Journal ArticleDOI
Irreversible binding of the chlorpromazine radical cation and of photoactivated chlorpromazine to biological macromolecules
N. J. De Mol,R.W. Busker +1 more
TL;DR: The irreversible binding of chlorpromazine radical cation (CPZ+.) and photoactivated chlor Promazine ( CPZ) to calf thymus DNA in vitro and bacterial macromolecules in intact bacterium cells was investigated and the consequences of covalent binding for the cytotoxicity and genotoxicity of CPZ+.
Journal ArticleDOI
Protein-ligand interactions: interaction of nitrosamines with nicotinic acetylcholine receptor.
TL;DR: Scatchard analysis indicates that all four ligands are true agonists of the receptor exhibiting positive cooperative binding with the existence of more than one class of binding site.
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