Journal ArticleDOI
Interaction of the Escherichia coli HU protein with DNA. Evidence for formation of nucleosome-like structures with altered DNA helical pitch.
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TLDR
The results suggest a model in which DNA is coiled in left-handed toroidal supercoils on the HU complex, having a stoichiometry resembling that of the half-nucleosome of eukaryotic chromatin.About:
This article is published in Journal of Molecular Biology.The article was published on 1986-01-05. It has received 305 citations till now. The article focuses on the topics: HU Protein & DNA supercoil.read more
Citations
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Bacterial nucleoid-associated proteins, nucleoid structure and gene expression
TL;DR: NAP biology is considered from the standpoints of both gene regulation and nucleoid structure to show that nucleoid-associated proteins (NAPs) and transcription contribute in combination to the dynamic nature of nucleoids structure.
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A physiological role for DNA supercoiling in the osmotic regulation of gene expression in S. typhimurium and E. coli
Christopher F. Higgins,Charles J. Dorman,Douglas A. Stirling,Lesley Waddell,Ian R. Booth,Gerhard May,Erhard Bremer +6 more
TL;DR: Evidence that DNA supercoiling plays a key role in the osmotic induction of proU transcription is presented, and mutations in osmZ are highly pleiotropic, affecting expression of a variety of chromosomal genes including ompF, ompC, fimA, and the bgl operon.
Journal ArticleDOI
Cyclic AMP in prokaryotes.
J. L. Botsford,J G Harman +1 more
TL;DR: This review summarizes recent work focused on elucidating the molecular mechanisms of CRP-cAMP complex-mediated processes in enteric coliforms and suggests a role for cAMP has been suggested in nitrogen fixation.
Journal ArticleDOI
Nucleotide excision repair in Escherichia coli.
TL;DR: Using a number of in vitro assays, researchers have been able to elucidate the action mechanism of the UvrABC nuclease complex, which represents a unique form of protein-DNA interaction.
References
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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
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A rapid, sensitive, and versatile assay for protein using Coomassie brilliant blue G250
TL;DR: An assay for proteins in solution that depends on the conversion of Coomassie brilliant blue G250 in dilute acid from a brownish-orange to an intense blue color has high reproducibility and can detect less than 1.0 μg of albumin.
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High resolution acrylamide gel electrophoresis of histones.
Sakol Panyim,Roger Chalkley +1 more
TL;DR: A high resolution gel electrophoresis of histone is described, capable of distinguishing between histone fractions whose mobilities differ by as little as 1% under the conditions of pH and urea concentration employed.
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Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis
TL;DR: The use of polyacrylamide gel electrophoresis to estimate chain lengths of double- and single-stranded DNA molecules in the size range 20-1000 base pairs (or nucleotides) is described.
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E. coli DNA binding protein HU forms nucleosome-like structure with circular double-stranded DNA
TL;DR: The incubation of the E coli DNA binding protein HU with relaxed circular SV40 DNA in the presence of pure nicking-closing enzyme introduces up to 18 negative superhelical turns in the DNA molecules as measured by agarose gel electrophoresis.