Investigation on cell proliferation with a new antibody against thymidine kinase 1.
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TLDR
A polyclonal anti‐TK1 antibody against a synthetic peptide from the C‐terminus of human TK1 is developed and demonstrated, demonstrating the exclusive location of TK 1 in the cytoplasm of cells.Abstract:
The cytosolic thymidine kinase 1 (TK1) is one of the enzymes involved in DNA replication. Based on biochemical studies, TK1 is activated at late G1 of cell cycle, and its activity correlates with the cell proliferation. We have developed a polyclonal anti‐TK1 antibody against a synthetic peptide from the C‐terminus of human TK1. Using this antibody, here we demonstrate the exclusive location of TK1 in the cytoplasm of cells. Cell cycle dependent TK1 expression was studied by simultaneous fluorescence staining for TK1 and bromodeoxyuridine, by using elutriated cells, and by quantitation of the amount TK1 in relation to the cellular DNA content. TK1, which was strongly expressed in the cells in S+G2 period, raised at late G1 and decreased during mitosis. The amount of TK1 increased three folds from late G1 to G2. TK1 positive cells were demonstrated in areas of proliferation activity of various normal and malignant tissues. The new anti‐TK1 antibody works in archival specimens and is a specific marker of cell proliferation.read more
Citations
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Measuring proliferation in breast cancer: practicalities and applications.
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Production and characterisation of a novel chicken IgY antibody raised against C-terminal peptide from human thymidine kinase 1.
Chuanjing Wu,Rongjiang Yang,Ji Zhou,Shing Bao,Li Zou,Pinggan Zhang,Yongrong Mao,Jianping Wu,Qimin He +8 more
TL;DR: Chicken egg yolk immunoglobulins generated to a synthetic 31-amino acid peptide from the C-terminal of human HeLa thymidine kinase 1 (TK1) enzyme are suggested to be potentially useful for serological and immunohistochemical detection of TK1 as an early prognosis and for monitoring patients undergoing treatment.
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TL;DR: The status of TK1 for cancer monitoring and its use as a proliferation marker are summarized and a comprehensive overview about the association of Tk-1 with various entities is given.
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TL;DR: The aim of the current study was to perform methodologic, descriptive, comparative, and prognostic studies of Ki‐67, proliferating cell nuclear antigen (PCNA), and flow cytometric S‐phase fraction in endometrial carcinoma.