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Journal ArticleDOI

Ion-exchange chromatography of tissue nucleotides.

01 Jan 1969-Journal of Chromatography A (Elsevier)-Vol. 44, Iss: 2, pp 331-341
TL;DR: The procedure gives a high degree of resolution of nucleotides in tissue extracts, and eluate samples can be freed of salt and water with a minimum of manipulation, by storage at reduced pressure and room temperature over sodium hydroxide and phosphorus pentoxide.
About: This article is published in Journal of Chromatography A.The article was published on 1969-01-01. It has received 62 citations till now. The article focuses on the topics: Triethylammonium acetate & Ion chromatography.
Citations
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Journal ArticleDOI
TL;DR: In this paper, a dual retention mechanism that postulates solute retention as a result of both solvophobic (hydrophobic) and silanophilic interactions between the eluite and stationary phase was proposed.

405 citations

Journal ArticleDOI
TL;DR: In this paper, a dual binding model was proposed to predict the temperature dependence of retention under various conditions as far as the nature of the stationary phase and the eluent composition are concerned.

396 citations

Journal ArticleDOI
TL;DR: Radiochromatography of nucleotides following intravenous injection of [14C]formate indicated a marked enhancement of postischemic purine de novo synthesis, which was partially re‐utilized by salvage pathways.
Abstract: —Complete cerebral ischemia was produced in normothermic anaesthetized cats by clamping the innominate and the left subclavian arteries combined with lowering the blood pressure. After 1 h of ischemia, ATP was no longer present in detectable amounts. Total adenine nucleotides were reduced to 34 per cent of the normal level. The breakdown of guanine nucleotides was less marked, with small amounts of GTP still being present at the end of the ischemic period. In animals with signs of functional recovery after 3–7 h of recirculation, ATP was resynthesized to 62 per cent of the control level. Total adenine nucleotides increased to 68 per cent and the adenylate energy change—[ATP + 1/2 ADP]/[AMP + ADP + ATP]—was re-established to within 7 per cent of the pre-ischemic value. Radiochromatography of nucleotides following intravenous injection of [14C]formate indicated a marked enhancement of postischemic purine de novo synthesis. Purine nucleosides and free bases which accumulated during ischemia, were partially re-utilized by salvage pathways: adenosine was rephosphorylated to AMP by adenosine kinase (EC 2.7.1.20); inosine and hypoxanthine were re-used via IMP in a reaction mediated by hypoxanthine phosphoribosyltransferase (EC 2.4.2.8).

185 citations

Journal ArticleDOI
TL;DR: The authors' results provide a strong correlation between in vivo and in vitro transcription of T7 by RNA polymerase, and predicted short initiation site sequences, including the first few bases of the RNA chains.

179 citations

Journal ArticleDOI
TL;DR: Findings suggest that control mechanisms can maintain uridine nucleotide levels in a normal range, even in the presence of strong inhibitors of de novo UMP biosynthesis.

79 citations

References
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Journal ArticleDOI
TL;DR: Fibroblasts cultured from patients completely deficient in hypoxanthine-guanine phosphoribosyltransferase activity were studied to determine the biochemical bases of the increased rate of purine biosynthesis de novo observed in such patients.

235 citations

Journal ArticleDOI
TL;DR: A new method of gradient elution analysis for routine chromatographic analysis of urinary ketosteroids is described in detail and its efficiency examined both theoretically and with the help of experimental data.

175 citations