Journal ArticleDOI
Iron fortification of rice seed by the soybean ferritin gene.
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TLDR
The rice seed-storage protein glutelin promoter, GluB-1, was used to drive expression of the soybean gene specifically in developing, self-pollinated seeds (T1 seeds) of transgenic plants, as confirmed by reverse transcription PCR analysis.Abstract:
To improve the iron content of rice, we have transferred the entire coding sequence of the soybean ferritin gene into Oryza sativa (L. cv. Kita-ake) by Agrobacterium-mediated transformation. The rice seed-storage protein glutelin promoter, GluB-1, was used to drive expression of the soybean gene specifically in developing, self-pollinated seeds (T1 seeds) of transgenic plants, as confirmed by reverse transcription PCR analysis. Stable accumulation of the ferritin subunit in the rice seed was demonstrated by western blot analysis, and its specific accumulation in the endosperm by immunologic tissue printing. The iron content of T1 seeds was as much as threefold greater than that of their untransformed counterparts.read more
Citations
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Enrichment of cereal grains with zinc: Agronomic or genetic biofortification?
TL;DR: Plant breeding strategy (e.g., genetic biofortification) appears to be a most sustainable and cost-effective approach useful in improving Zn concentrations in grain, and application of Zn fertilizers or Zn-enriched NPK fertilizers offers a rapid solution to the problem.
Journal ArticleDOI
Biofortification of crops with seven mineral elements often lacking in human diets--iron, zinc, copper, calcium, magnesium, selenium and iodine.
TL;DR: In this paper, the authors review aspects of soil science, plant physiology and genetics underpinning crop bio-fortification strategies, as well as agronomic and genetic approaches currently taken to biofortify food crops with the mineral elements most commonly lacking in human diets: iron (Fe), zinc (Zn), copper (Cu), calcium (Ca), magnesium (Mg), iodine (I) and selenium (Se).
Journal ArticleDOI
Nutritional iron deficiency
TL;DR: Although technical challenges limit the amount of bioavailable iron compounds that can be used in food fortification, studies show that iron fortification can be an effective strategy against nutritional iron deficiency.
Journal ArticleDOI
The ZIP family of metal transporters.
TL;DR: Information on where in the plant each of the ZIP transporters functions and how each is controlled in response to nutrient availability may allow the manipulation of plant mineral status with an eye to creating food crops with enhanced mineral content, and developing crops that bioaccumulate or exclude toxic metals.
Journal ArticleDOI
Functional analysis of a NAC-type transcription factor OsNAC6 involved in abiotic and biotic stress-responsive gene expression in rice
Kazuo Nakashima,Lam-Son Phan Tran,Dong Van Nguyen,Miki Fujita,Kyonoshin Maruyama,Daisuke Todaka,Yusuke Ito,Nagao Hayashi,Kazuo Shinozaki,Kazuko Yamaguchi-Shinozaki +9 more
TL;DR: In this article, a transactivation assay using a yeast system demonstrated that OsNAC6 functions as a transcriptional activator, and transient localization studies with OsAC6-sGFP fusion protein revealed its nuclear localization.
References
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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
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Standard methods for the examination of water and wastewater
TL;DR: The most widely read reference in the water industry, Water Industry Reference as discussed by the authors, is a comprehensive reference tool for water analysis methods that covers all aspects of USEPA-approved water analysis.
Journal ArticleDOI
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more
TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
Journal ArticleDOI
Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources
Chih-Ching Chu,Ching-Chu Wang,Ching-San Sun,Chen Hsu,Kwang-Chu Yin,Chih--Yin Chu,Feng-Yun Bi +6 more
TL;DR: In this paper, it was shown that (NH4)2SO4 combined with KNO3 at low concentration is of advantage to the formation, growth and differentiation of pollen callus in rice.