Iron Transport in Salmonella typhimurium: Mutants Blocked in the Biosynthesis of Enterobactin
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TLDR
Transduction experiments showed that the genes for enterobactin biosynthesis are closely linked on the S. typhimurium chromosome, and it is suggested that they form an operon which is repressed by the presence of iron.Abstract:
A number of mutants of Salmonella typhimurium were isolated which are blocked in the biosynthesis of enterobactin, an iron chelator that is secreted by the wild-type bacteria when they are grown on low iron media One class of these enb mutants accumulates the enterobactin precursor 2,3-dihydroxybenzoic acid, and another class does not accumulate any detectable catechol precursor The enb mutants grow very well on a glucose-mineral salts medium Addition of citrate, itself an iron chelator, to the medium drastically inhibits growth unless the medium is supplemented with enterobactin or iron salts Citrate inhibits iron uptake from the medium by enb mutants; enterobactin counteracts this inhibition and also, by itself, increases iron uptake Thus, the apparent function of enterobactin is to promote the absorption of iron from the medium by the bacteria Transduction experiments showed that the genes for enterobactin biosynthesis are closely linked on the S typhimurium chromosome It is suggested that they form an operon which is repressed by the presence of iron S typhimurium can utilize the iron chelate ferrichrome (Deferriferrichrome is a cyclic hexapeptide that is produced by some fungi but not by S typhimurium) The enb mutants use ferrichrome as an effective growth factorread more
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Linkage map of Salmonella typhimurium, edition VII.
TL;DR: Presentation de la sixieme carte genetique de Salmonella typhimurium comprenant 532 genes, plus de quelques nouvelles methodes et de nouveaux materiels pour l'analyse genetiques.
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Molecular mechanism of regulation of siderophore-mediated iron assimilation.
A Bagg,J B Neilands +1 more
TL;DR: This review describes a molecular mechanism for regulation of one pathway of microbial assimilation of iron, an element which may well be a nutrient required by all living cells and which cannot be synthesized and must be acquired from the environment.
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Genetics and molecular biology of siderophore-mediated iron transport in bacteria.
TL;DR: The potential role of siderophore-mediated systems as virulence determinants in the specific host-bacteria interaction leading to disease is analyzed with respect to the influence of these systems in the expression of other factors, such as toxins, in the bacterial virulence repertoire.
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Stoffwechselprodukte von Mikroorganismen
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References
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Acetylornithinase of Escherichia coli: partial purification and some properties.
Henry J. Vogel,David M. Bonner +1 more
TL;DR: Compounds Used-N”l-Acetyl-n-ornithine was synthesized as previously described and L-Ornithine monohydrochloride was obtained from the Mann Research Laboratories.
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Enterobactin, an iron transport compound from Salmonella typhimurium.
J.R. Pollack,J.B. Neilands +1 more
TL;DR: Enterobactin, a physiologically active iron sequestering agent, is excreted into the medium by Salmonella typhimurium under low iron conditions and is a cyclic polyester consisting of three residues of 2,3-dihydroxy-N-benzoylserine.
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TL;DR: A mutually noncomplementing group of phage P22 mutants (L mutants) have been isolated which cannot accomplish a late step necessary for prophage integration, and the L+ gene is thus necessary for integration but not for maintenance of the lysogenic state.
Journal ArticleDOI
The linkage map of salmonella typhimurium.
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