Isolation and Characterization of a myo-inositol-1-phosphate Synthase Gene from Yellow Passion Fruit (Passiflora edulis f. flavicarpa) Expressed During Seed Development and Environmental Stress
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Cites methods from "Isolation and Characterization of a..."
...PCRs were carried out as described [ 62 ], except that 20 ng of cDNA was used as a template, in reactions with 32 cycles of amplification....
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152 citations
Cites background from "Isolation and Characterization of a..."
...Myo-inositol-1-phosphate synthase is a key rate limiting enzyme of inositol biosynthesis (Abreu and Arag~ao, 2007)....
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...4) is a key rate limiting enzyme of myo-inositol biosynthesis which catalyses the reaction from glucose-6-phosphate (G-6-P) to myo-inositol-1-phosphate (Ins1P), which is subsequently dephosphorylated by myo-inositol monophosphatase (MIPP) to form free inositol (Abreu and Arag~ao, 2007)....
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121 citations
Cites background from "Isolation and Characterization of a..."
...MIPS catalyses the conversion of D-glucose 6-phosphate to 1-myo-inositol-1phosphate, the first and rate-limiting step in the biosynthesis of all inositol containing compounds (Abreu and Aragao 2007)....
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References
63,427 citations
"Isolation and Characterization of a..." refers methods in this paper
...The alignment was performed using CLUSTAL W (Thompson et al., 1994)....
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57,055 citations
"Isolation and Characterization of a..." refers methods in this paper
...Phylogenetic trees were then constructed using the neighbour-joining algorithm (Saitou and Nei, 1987)....
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26,016 citations
"Isolation and Characterization of a..." refers methods in this paper
...Thus, to consider this unequal probability of the substitution types, the Kimura 2-parameter (Kimura, 1980) was used to compute distances between each pair of sequences....
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12,124 citations
"Isolation and Characterization of a..." refers methods in this paper
...Phylogenetic analyses were carried out using the MEGA (Molecular Evolutionary Genetic Analysis) version 3.1 software program (Kumar et al., 2004)....
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4,268 citations
"Isolation and Characterization of a..." refers background in this paper
...The TargetP 1.1 (Emanuelsson et al., 2000) and ChloroP (Emanuelsson et al., 1999) program algorithms predicted no signal, chloroplast transit or mitochondrial targeting peptides in the N-terminal region of the PeMIPS1....
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