Journal ArticleDOI
Isolation and Characterization of a Near-Haploid Human Cell Line
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TLDR
By serial subcloning, a near-haploid subclone (P1-55) is isolated from a heterogeneous human leukemia cell line, KBM-7, which has approximately half the human diploid DNA content and has a haploid karyotype except for a disomy of chromosome 8.About:
This article is published in Experimental Cell Research.The article was published on 1999-11-01. It has received 136 citations till now. The article focuses on the topics: Karyotype & Ploidy.read more
Citations
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Journal ArticleDOI
High-throughput functional genomics using CRISPR–Cas9
TL;DR: A review of the latest applications of CRISPR-Cas9 in mammalian functional genomics screens is presented in this article, which covers related genome-scale applications of Cas9 for either gene knockout or transcriptional modulation.
Journal ArticleDOI
Haploid Genetic Screens in Human Cells Identify Host Factors Used by Pathogens
Jan E. Carette,Carla P. Guimaraes,Malini Varadarajan,Annie S. Park,Irene Wuethrich,Alzbeta Godarova,Maciej Kotecki,Brent H. Cochran,Eric Spooner,Hidde L. Ploegh,Thijn R. Brummelkamp +10 more
TL;DR: Using insertional mutagenesis to develop a screening method to generate null alleles in a human cell line haploid for all chromosomes except chromosome 8, host factors essential for infection with influenza and genes encoding important elements of the biosynthetic pathway of diphthamide, which are required for the cytotoxic effects ofdiphtheria toxin and exotoxin A are identified.
Journal ArticleDOI
Caspase-mediated cleavage of phospholipid flippase for apoptotic phosphatidylserine exposure
Katsumori Segawa,Sachiko Kurata,Yuichi Yanagihashi,Thijn R. Brummelkamp,Fumihiko Matsuda,Shigekazu Nagata +5 more
TL;DR: Using a haploid genetic screen in human cells, it is found that ATP11C and CDC50A are required for aminophospholipid translocation from the outer to the inner plasma membrane leaflet; that is, they display flippase activity.
Journal ArticleDOI
Genome-wide Maps of Nuclear Lamina Interactions in Single Human Cells
Jop Kind,Jop Kind,Ludo Pagie,Sandra S. de Vries,Leila Nahidiazar,Siddharth S. Dey,Magda Bienko,Ye Zhan,Bryan R. Lajoie,Carolyn A. de Graaf,Mario Amendola,Geoffrey Fudenberg,Maxim Imakaev,Leonid A. Mirny,Kees Jalink,Job Dekker,Alexander van Oudenaarden,Bas van Steensel +17 more
TL;DR: The consistency of NL contacts is inversely linked to gene activity in single cells and correlates positively with the heterochromatic histone modification H3K9me3, which highlights fundamental principles of single-cell chromatin organization.
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Obligate ligation-gated recombination (ObLiGaRe): custom-designed nuclease-mediated targeted integration through nonhomologous end joining.
TL;DR: ObLiGaRe is described, a new method for site-specific gene insertions that uses the efficient NHEJ pathway and acts independently of HR, and has enabled us to insert a 15-kb inducible gene expression cassette at a defined locus in human cell lines.
References
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Journal ArticleDOI
Altering the genome by homologous recombination.
TL;DR: The current status of gene targeting with particular emphasis on germ line modification of the mouse genome is discussed, and the different methods so far employed to identify those rare embryonic stem cells in which the desired targeting event has occurred are described.
Journal ArticleDOI
Production of clones of homozygous diploid zebra fish ( Brachydanio rerio )
TL;DR: Clones of homozygous fish have been produced from individual homozygotes and associated genetic methods facilitate genetic analyses of this vertebrate.
Journal ArticleDOI
Bypass of Senescence After Disruption of p21CIP1/WAF1 Gene in Normal Diploid Human Fibroblasts
TL;DR: At the checkpoint between the prereplicative phase of growth and the phase of chromosome replication, cells lacking p21 failed to arrest the cell cycle in response to DNA damage, but their apoptotic response and genomic stability were unaltered.
Journal Article
Phenotypes of c-Myc-deficient rat fibroblasts isolated by targeted homologous recombination
TL;DR: The c-myc null cell lines reported here are a new experimental system in which to investigate the importance of putative c-Myc target genes and to identify novel downstream genes involved in cell cycle progression and apoptosis.
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