Journal ArticleDOI
Isolation and Characterization of a Near-Haploid Human Cell Line
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TLDR
By serial subcloning, a near-haploid subclone (P1-55) is isolated from a heterogeneous human leukemia cell line, KBM-7, which has approximately half the human diploid DNA content and has a haploid karyotype except for a disomy of chromosome 8.About:
This article is published in Experimental Cell Research.The article was published on 1999-11-01. It has received 136 citations till now. The article focuses on the topics: Karyotype & Ploidy.read more
Citations
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Journal ArticleDOI
Hunting Viral Receptors Using Haploid Cells
Sirika Pillay,Jan E. Carette +1 more
TL;DR: The most commonly employed methods for virus receptor discovery are discussed, specifically highlighting the use of forward genetic screens in human haploid cells and the discovery of the lysosomal proteins NPC1 and LAMP1 as intracellular receptors for Ebola virus and Lassa virus.
Journal ArticleDOI
Genome wide functional genetics in haploid cells
Ulrich Elling,Josef M. Penninger +1 more
TL;DR: Haploid genetics has thus become a powerful alternative to RNAi or CRISPR based screens and open the possibility of combining the power of a haploid genome with pluripotency of embryonic stem cells to uncover fundamental biological processes in defined cell types at a genomic scale.
Journal ArticleDOI
Haploidy in Humans: An Evolutionary and Developmental Perspective
Ido Sagi,Nissim Benvenisty +1 more
TL;DR: Haploidy in evolution and the barriers to haploidy, in particular in the human context are discussed and major differences in cell size and gene expression levels are reported.
Journal ArticleDOI
Quantitative live-cell imaging and computational modeling shed new light on endogenous WNT/CTNNB1 signaling dynamics.
TL;DR: Wang et al. as discussed by the authors combined CRISPR/Cas9-mediated genome editing and quantitative live-cell microscopy to measure the dynamics, diffusion characteristics and absolute concentrations of fluorescently tagged, endogenous CTNNB1 in human cells under both physiological and oncogenic conditions.
Journal ArticleDOI
Efficient and crucial quality control of HAP1 cell ploidy status.
TL;DR: Sharing an effective procedure to quality control the near-haploid HAP1 cells for standardized comparison to CRISPR/Cas9 modified versions and demonstrating the need for controlling the spontaneous diploidization of H AP1 cultures is shared.
References
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Journal ArticleDOI
Altering the genome by homologous recombination.
TL;DR: The current status of gene targeting with particular emphasis on germ line modification of the mouse genome is discussed, and the different methods so far employed to identify those rare embryonic stem cells in which the desired targeting event has occurred are described.
Journal ArticleDOI
Production of clones of homozygous diploid zebra fish ( Brachydanio rerio )
TL;DR: Clones of homozygous fish have been produced from individual homozygotes and associated genetic methods facilitate genetic analyses of this vertebrate.
Journal ArticleDOI
Bypass of Senescence After Disruption of p21CIP1/WAF1 Gene in Normal Diploid Human Fibroblasts
TL;DR: At the checkpoint between the prereplicative phase of growth and the phase of chromosome replication, cells lacking p21 failed to arrest the cell cycle in response to DNA damage, but their apoptotic response and genomic stability were unaltered.
Journal Article
Phenotypes of c-Myc-deficient rat fibroblasts isolated by targeted homologous recombination
TL;DR: The c-myc null cell lines reported here are a new experimental system in which to investigate the importance of putative c-Myc target genes and to identify novel downstream genes involved in cell cycle progression and apoptosis.
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