Journal ArticleDOI
Isolation and Characterization of a Near-Haploid Human Cell Line
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TLDR
By serial subcloning, a near-haploid subclone (P1-55) is isolated from a heterogeneous human leukemia cell line, KBM-7, which has approximately half the human diploid DNA content and has a haploid karyotype except for a disomy of chromosome 8.About:
This article is published in Experimental Cell Research.The article was published on 1999-11-01. It has received 136 citations till now. The article focuses on the topics: Karyotype & Ploidy.read more
Citations
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Journal ArticleDOI
Haploid genomes illustrate epigenetic constraints and gene dosage effects in mammals
Martin Leeb,Anton Wutz +1 more
TL;DR: The recent literature on haploid genomes and dosage effects is reviewed and it is tried to embed recent findings in an evolutionary perspective.
Posted ContentDOI
Biological Plasticity Rescues Target Activity in CRISPR Knockouts
Arne H. Smits,Frederik Ziebell,Gerard Joberty,Nico Zinn,William F. Mueller,Sandra Clauder-Münster,Dirk Eberhard,M. Fälth Savitski,Paola Grandi,Petra Jakob,A. M. Michon,Han Sun,K. Tessmer,Tilmann Bürckstümmer,Marcus Bantscheff,Lars M. Steinmetz,Gerard Drewes,Wolfgang Huber +17 more
TL;DR: An experimental strategy combining RNA-seq and triple-stage mass spectrometry to characterize 193 genetically verified deletions targeting 136 distinct genes generated by CRISPR-induced frameshifts in HAP1 cells implies that systematic characterization of residual protein expression or function in CRISpr-Cas9 generated KO lines is necessary for phenotype interpretation.
Patent
Compositions and methods for mammalian genetics and uses thereof
TL;DR: In this paper, the authors provide compositions and methods for performing mammalian cell genetics, e.g., genetic screens, using near-haploid cells, and further provide genes and gene products isolated using the inventive methods and methods of use thereof.
Journal ArticleDOI
'Artificial spermatid'-mediated genome editing†.
TL;DR: DKO-AG-haESCs combed with CRISPR-Cas technology can be used as the genetically tractable fertilization agent, to efficiently create genetically modified offspring, and is a versatile genetic tool for in vivo analyses of gene function.
Book ChapterDOI
Higher-Order Chromatin Organization in Diseases: From Chromosomal Position Effect to Phenotype Variegation
TL;DR: Common themes are emerging from the review of fundamental research and clinical observations, which are the focus of this chapter.
References
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Journal ArticleDOI
Altering the genome by homologous recombination.
TL;DR: The current status of gene targeting with particular emphasis on germ line modification of the mouse genome is discussed, and the different methods so far employed to identify those rare embryonic stem cells in which the desired targeting event has occurred are described.
Journal ArticleDOI
Production of clones of homozygous diploid zebra fish ( Brachydanio rerio )
TL;DR: Clones of homozygous fish have been produced from individual homozygotes and associated genetic methods facilitate genetic analyses of this vertebrate.
Journal ArticleDOI
Bypass of Senescence After Disruption of p21CIP1/WAF1 Gene in Normal Diploid Human Fibroblasts
TL;DR: At the checkpoint between the prereplicative phase of growth and the phase of chromosome replication, cells lacking p21 failed to arrest the cell cycle in response to DNA damage, but their apoptotic response and genomic stability were unaltered.
Journal Article
Phenotypes of c-Myc-deficient rat fibroblasts isolated by targeted homologous recombination
TL;DR: The c-myc null cell lines reported here are a new experimental system in which to investigate the importance of putative c-Myc target genes and to identify novel downstream genes involved in cell cycle progression and apoptosis.
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