Journal ArticleDOI
Isolation and Characterization of a Near-Haploid Human Cell Line
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TLDR
By serial subcloning, a near-haploid subclone (P1-55) is isolated from a heterogeneous human leukemia cell line, KBM-7, which has approximately half the human diploid DNA content and has a haploid karyotype except for a disomy of chromosome 8.About:
This article is published in Experimental Cell Research.The article was published on 1999-11-01. It has received 136 citations till now. The article focuses on the topics: Karyotype & Ploidy.read more
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Posted ContentDOI
Nascent RNA sequencing of peripheral blood leukocytes reveal gene expression diversity
TL;DR: UPRO and pChRO provided a powerful platform to identify differentially expressed genes between individuals with minimal sample requirements and generated genome-wide transcription profiles of human haploid cell lines and peripheral blood samples combined with Chromatin Run-On sequencing.
Journal ArticleDOI
Dissection of splicing regulation at an endogenous locus by zinc-finger nuclease-mediated gene editing.
TL;DR: Using a ZFN pair along with a donor plasmid to manipulate the outcomes of DNA repair, several discrete, targeted mutations are introduced into the fourth intron of the endogenous BAX gene in Chinese hamster ovary cells demonstrating that ZFN-mediated gene editing is a highly effective tool for dissection of pre-mRNA splicing regulatory sequences in their endogenous context.
Journal ArticleDOI
Use of a genome-wide haploid genetic screen to identify treatment predicting factors: a proof-of-principle study in pancreatic cancer.
Yuk Ting Ma,Sarah Leonard,Naheema S. Gordon,Jennifer Anderton,Claire D. James,David Huen,Ciaran B J Woodman,Daniel H. Palmer +7 more
TL;DR: It is shown that in human pancreatic cancer cell lines, treatment with the potent class I histone deacetylase inhibitor, entinostat, synergistically enhances gemcitabine-induced inhibition of cell proliferation and apoptosis.
Journal ArticleDOI
Comprehensive analysis of DNA replication timing across 184 cell lines suggests a role for <i>MCM10</i> in replication timing regulation
TL;DR: In this paper , the authors identify trans-acting modulators of DNA replication timing in 184 cell lines from three cell types, encompassing 60 different gene knockouts or genetic diseases.
Patent
Mammalian haploid embryonic stem cells
Anton Wutz,Martin Leeb +1 more
TL;DR: In this paper, a cell culture and a cell line of mammalian haploid embryonic stem cells were described and a method for the production of such stem cells was described. But this method is not applicable to the development of artificial neural networks.
References
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Journal ArticleDOI
Altering the genome by homologous recombination.
TL;DR: The current status of gene targeting with particular emphasis on germ line modification of the mouse genome is discussed, and the different methods so far employed to identify those rare embryonic stem cells in which the desired targeting event has occurred are described.
Journal ArticleDOI
Production of clones of homozygous diploid zebra fish ( Brachydanio rerio )
TL;DR: Clones of homozygous fish have been produced from individual homozygotes and associated genetic methods facilitate genetic analyses of this vertebrate.
Journal ArticleDOI
Bypass of Senescence After Disruption of p21CIP1/WAF1 Gene in Normal Diploid Human Fibroblasts
TL;DR: At the checkpoint between the prereplicative phase of growth and the phase of chromosome replication, cells lacking p21 failed to arrest the cell cycle in response to DNA damage, but their apoptotic response and genomic stability were unaltered.
Journal Article
Phenotypes of c-Myc-deficient rat fibroblasts isolated by targeted homologous recombination
TL;DR: The c-myc null cell lines reported here are a new experimental system in which to investigate the importance of putative c-Myc target genes and to identify novel downstream genes involved in cell cycle progression and apoptosis.
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