Isolation and characterization of a new keratinolytic bacterium that exhibits significant feather-degrading capability
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TLDR
Potential biotechnological applications of this bacterium that involve hydrolysis of keratin, including the improvement of the nutritional properties of feathers (and other keratins) used as supplementary feedstuffs are suggested.Abstract:
A novel bacterium, Bacillus licheniformis K-19, which produces a large amount of akeratinase that is extremely thermostable and has a broad resistance to pH, was isolated and characterized. The maximum amount of keratinase activity (about 224 Uml-1) was produced at 37°C when the bacterium was cultured for 72 h in broth containing feather meal with initial pH of 7.5. The keratinase activity was observed over a wide range of temperatures (30 - 90°C) and pH values (pH 6 - 10). It was optimal at 60°C and pH 7.5 - 8 respectively. These results suggest potential biotechnological applications of this bacterium that involve hydrolysis of keratin, including the improvement of the nutritional properties of feathers (and other keratins) used as supplementary feedstuffs.
Key words: Bacillus licheniformis, chicken feather, keratin, keratinolytic protease.read more
Citations
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TL;DR: The isolated strain of keratinase-producing bacterium was thermotolerant and alkalophilic bacterium which can therefore be used in leather processing and detergent industry and did not inhibit the enzyme.
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Keratinolytic activities of alkaliphilic Bacillus sp. MBRL 575 from a novel habitat, limestone deposit site in Manipur, India
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Identification and characterization of feather-degrading bacteria from keratin-rich wastes
Younes Ghasemi,Maryam Shahbazi,Maryam Shahbazi,Sara Rasoul-Amini,Mohammad Kargar,Azam Safari,Aboozar Kazemi,Nima Montazeri-Najafabady +7 more
TL;DR: The thermostability of this enzyme makes it feasible to take advantage of this bacterium in biotechnological processes, and the highest keratinolytic activity exhibited in this strain was selected for further characterization.
References
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Journal ArticleDOI
Purification and Characterization of a Keratinase from a Feather-Degrading Bacillus licheniformis Strain
TL;DR: The purified keratinase hydrolyzes a broad range of substrates and displays higher proteolytic activity than most proteases and is a useful enzyme for promoting the hydrolysis of feather keratin and improving the digestibility of feather meal.
Journal ArticleDOI
Isolation, identification, and characterization of a feather-degrading bacterium.
TL;DR: A feather-degrading culture was enriched with isolates from a poultry waste digestor and adapted to grow with feathers as its primary source of carbon, sulfur, and energy, indicating a potential biotechnique for degradation and utilization of feather keratin.
Journal Article
The use of azoalbumin as a substrate in the colorimetric determination or peptic and tryptic activity.
Journal ArticleDOI
Characterization of a new keratinolytic bacterium that completely degrades native feather keratin.
TL;DR: A novel feather-degrading microorganism was isolated from poultry waste, producing a high keratinolytic activity when cultured on broth containing native feather, and complete feather degradation was achieved during cultivation.
Journal ArticleDOI
Native-feather degradation by Fervidobacterium islandicum AW-1, a newly isolated keratinase-producing thermophilic anaerobe
Gae Won Nam,Dong Woo Lee,Han Seoung Lee,Nam Lee,Byoung-Chan Kim,Eun Ah Choe,Jae Kwan Hwang,Maggy Thenawidjaja Suhartono,Yu Ryang Pyun +8 more
TL;DR: The enzyme from F. islandicum AW-1 is a novel, thermostable keratinolytic serine protease that showed higher specific activity for the keratinous substrates than other proteases and catalyzed the cleavage of peptide bonds more rapidly following the reduction of disulfide bridges in feather keratin by 10 mM dithiothreitol.
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