Journal ArticleDOI
Isolation and characterization of plasminogen and plasmin from bovine milk
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TLDR
Enzyme-linked immunosorbent assay (ELISA) and Western blotting experiments revealed that immunoreactive plasminogen was associated with acid-precipitated casein, rennet-coagulated casein and casein micelles, and was found in acid whey and to a lesser extent in rennet whey.About:
This article is published in International Dairy Journal.The article was published on 1995-01-01. It has received 79 citations till now. The article focuses on the topics: Plasmin & Casein.read more
Citations
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The proteolytic systems of lactic acid bacteria
TL;DR: A model for the proteolytic pathway is proposed that consists of an extracellularly located serine-proteinase, transport systems specific for di-tripeptides and oligopeptides, and a multitude of intracellular peptidases.
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A review and proposed nomenclature for major proteins of the milk-fat globule membrane.
TL;DR: A revised nomenclature for the major membrane proteins is proposed and discussed in relation to earlier schemes, and it is recommended that proteins be assigned specific names as they are identified by molecular cloning and sequencing techniques.
Journal ArticleDOI
Plasmin in milk and dairy products: an update.
Eric D. Bastian,Rodney J. Brown +1 more
TL;DR: Plasmin is secreted as plasminogen that is activated in blood and milk and its role in blood is to proteolytically break down blood clots.
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Detection and impact of protease and lipase activities in milk and milk powders
TL;DR: In this paper, the authors focus on the characteristics of proteases (specifically proteinases) and lipases in milk and milk powders, detection methods for such enzymes and the effects of these enzymes on milk during storage.
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Inhibitory effects of human and bovine milk constituents on rotavirus infections.
A.S. Kvistgaard,Lone Tjener Pallesen,Carlos F. Arias,Susana López,Torben E. Petersen,Christian W. Heegaard,Jan T. Rasmussen +6 more
TL;DR: It is shown that human, and not bovine, lactadherin inhibits Wa rotavirus infection in vitro, and a bovina macromolecular whey protein fraction turned out to have an efficient and versatile inhibitory activity against rotav virus.
References
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Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes.
TL;DR: Small amounts of myoglobin, beta-lactoglobulin, and other proteins and peptides can be spotted or electroblotted onto polyvinylidene difluoride membranes, stained with Coomassie Blue, and sequenced directly, suggesting that PVDF membranes are superior supports for sequence analysis of picomole quantities of proteins purified by gel electrophoresis.
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Plasminogen: Purification from Human Plasma by Affinity Chromatography
Dale G. Deutsch,Edwin T. Mertz +1 more
TL;DR: Plasminogen was prepared from human plasma by affinity chromatography on L-lysine-substituted Sepharose with a specific activity of 100 caseinolytic units per milligram of nitrogen.
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A simplified method for cyanogen bromide activation of agarose for affinity chromatography
TL;DR: Agarose can be activated by adding cyanogen bromide, dissolved in acetonitrile, to beads suspended in a solution of sodium carbonate, and the necessity for manual titration and the use of a pH meter are eliminated.