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Open AccessJournal ArticleDOI

Isolation of a Nonpigmented, Thermophilic Bacterium Similar to Thermus aquaticus

Robert F. Ramaley, +1 more
- 01 Aug 1970 - 
- Vol. 103, Iss: 2, pp 527-528
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TLDR
A thermophilic (70 C), gram-negative bacterium has been isolated that resembles Thermus aquaticus but lacks the type-characteristic yellow carotenoid.
Abstract
A thermophilic (70 C), gram-negative bacterium has been isolated that resembles Thermus aquaticus but lacks the type-characteristic yellow carotenoid.

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Citations
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A comparative analysis of extreme thermophilic bacteria belonging to the genus Thermus

TL;DR: Several extreme thermophilic Gram negative bacteria found in a thermally polluted river in Belgium have been compared with Thermus strains isolated from widely distant geographical areas, finding them to be morphologically identical with strain YT-1 of Thermus aquaticus.
Journal ArticleDOI

Genetic transformation of the extreme thermophile Thermus thermophilus and of other Thermus spp.

TL;DR: Competence was maintained throughout the growth phase, with the highest transformation frequencies at pH 6 to 9 and at 70 degrees C, and optimal transformation was obtained by the addition of a divalent cation (Ca2+ or Mg2+).
Journal ArticleDOI

Rhodothermus marinus, gen. nov., sp. nov., a Thermophilic, Halophilic Bacterium from Submarine Hot Springs in Iceland

TL;DR: Thermophilic, reddish-coloured heterotrophic bacteria different from Thermus were isolated from submarine alkaline hot springs in Iceland, considered to represent a new genus which is named Rhodothermus, with the type species Rhodother Mus marinus.
Journal ArticleDOI

The Deinococcus-Thermus phylum and the effect of rRNA composition on phylogenetic tree construction

TL;DR: Through comparative analysis of 16S ribosomal RNA sequences, it can be shown that two seemingly dissimilar types of eubacteria Deinococcus and the ubiquitous hot spring organism Thermus are distantly but specifically related to one another.
Journal ArticleDOI

Isolation and Characterization of Acidothermus cellulolyticus gen. nov., sp. nov., a New Genus of Thermophilic, Acidophilic, Cellulolytic Bacteria

TL;DR: The organisms are resistant to penicillin G at 100 μg/ml and share several important features with Thermus strains, namely heterotrophic, aerobic, and thermophilic mode of growth; morphological features; sensitivity to lysozyme; and presence of catalase.
References
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Journal ArticleDOI

A procedure for the isolation of deoxyribonucleic acid from micro-organisms

TL;DR: A method has been described for the isolation of DNA from micro-organisms which yields stable, biologically active, highly polymerized preparations relatively free from protein and RNA, and Representative samples have been characterized for their thermal stability and sedimentation behaviour.
Journal ArticleDOI

Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl.

TL;DR: A comprehensive study of the buoyant density of DNA as a function of composition has been made and the linear relation previously reported has been confirmed.
Journal ArticleDOI

Thermus aquaticus gen. n. and sp. n., a Nonsporulating Extreme Thermophile

TL;DR: Thermus aquaticus gen. n and sp. n are gram-negative nonsporulating nonmotile rods which frequently form long filaments at supraoptimal temperatures or in the stationary phase as mentioned in this paper.
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