Isolation of brain strandin, a new type of large molecule tissue component.
TL;DR: The isolation from brain tissue of a substance to which the name of strandin has been given for descriptive purposes is reported, which has the property of forming long strands that show perfect orientation under polarized light.
About: This article is published in Journal of Biological Chemistry.The article was published on 1951-08-01 and is currently open access. It has received 253 citations till now.
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TL;DR: In this paper, the authors described a simplified version of the method and reported the results of a study of its application to different tissues, including the efficiency of the washing procedure in terms of the removal from tissue lipides of some non-lipide substances of special biochemical interest.
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TL;DR: A simple method is described for the preparation of extracts of total pure lipides from brain tissue by homogenizing the tissue with a chloroform-methanol mixture and washing free of non-lipide contaminants.
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633 citations
References
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TL;DR: The acetylation of glucosamine which yields the Nmonoacetyl derivative, under the conditions described by Zuckerkandl and Messiner-Klebermass does not appear to be quantitative and rapid fading of the colour that develops on the addition of the p-dimethylaminobenzaldehyde reagent increases the difficulty of colorimetric estimation.
Abstract: A COLORIMETRIC method for the determination of glucosamine has recently been described by Zuckerkandl and Messiner-Klebermass [1931]. According to their procedure the test-solution, which should contain between 1 and 4 mg. glucosamine hydrochloride, is evaporated to dryness and treated with a freshly prepared solution of sodium methoxide. The free glucosamine base is then cautiously acetylated with acetic anhydride to yield the N-monoacetyl derivative which can be estimated by means of the intense reddish-purple colour that develops when, after a preliminary treatment with dilute alkali, p-dimethylaminobenzaldehyde in acid solution (Ehrlich's reagent) is added. We have found this method unsatisfactory. The acetylation of glucosamine which yields the Nmonoacetyl derivative, under the conditions described by Zuckerkandl and Messiner-Klebermass does not appear to be quantitative and, moreover, rapid fading of the colour that develops on the addition of the p-dimethylaminobenzaldehyde reagent increases the difficulty of colorimetric estimation. That Zuckerkandl and Messiner-Klebermass were aware of the difficulties involved in the use of the method for quantitative purposes is evident from their description of the reaction. They state that the error of estimation is, at the most, 6 %. CH-C. CO. OC2H8 H H OH
1,533 citations
TL;DR: A simple method is described for the preparation of extracts of total pure lipides from brain tissue by homogenizing the tissue with a chloroform-methanol mixture and washing free of non-lipide contaminants.
1,203 citations
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