Isolation of Piperin from The Fruit of Piper retrofractum
06 Mar 2017-Vol. 2, Iss: 1, pp 6-9
TL;DR: In this paper, the authors described the isolation of major compound from MeOH extract from the fruit of Piper retrofractum using several chromatography technique including liquid vacuum chromatography and thin layer chromatography, and further purification using re-cristalization technique.
Abstract: This paper will described the isolation of major compound from MeOH extract from the fruit of Piper retrofractum . Using several chromatography technique including liquid vacuum chromatography and thin layer chromatography, and further purification using re-cristalization technique, Piperine, an alkaloids compound, was isolated from this extract. The structure of this compound was determined using spectroscopic methods including FTIR, 1D-NMR and 2-D NMR. Keywords : P.retrofractum , alkaloids, piperine, structure elucidation
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TL;DR: Results indicate that P. retrofractum extract has great potential to be developed as a botanical insecticide for controlling BPH.
Abstract: Brown planthopper (Nilaparvata lugens; BPH) is one of major insect pests of rice and farmers often resort to synthetic insecticides to control this pest. Intensive and excessive use of synthetic insecticides cause several deleterious effects to human, beneficial organisms as well as the environment. Therefore, an alternative control strategy of BPH which is environmentally friendly and safer than the synthetic insecticides should be developed, such as botanical insecticides.This research sought to study mortality, feeding inhibition, and oviposition deterrent activities of Piper retrofractum, P. crocatum, Chromolaema odorata, Tagetes erecta, Tithonia diversifolia and Ageratum conyzoides extracts against BPH. Probit analysis was used to estimate LC50 and LC95 values of the extracts. P. retrofractrum extract exhibited highest mortality compared to the other extracts with LC95 values of 0.71%, followed by T. erecta 3.28%. The extract of P. retrofractum also indicated the highest feeding inhibition of 86.40% at LC75 value, which gradually decreased on T. erecta, T. diversifolia, P. crocatum, A. conyzoides, and C. odorata extracts were 72.68, 71.92, 68.89, 68.25, and 65.34%, respectively. The treatment of P. retrofractum extract at LC75 level caused the highest oviposition deterrence against BPH at 80.40%. All of the above results indicate that P. retrofractum extract has great potential to be developed as a botanical insecticide for controlling BPH.
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28 Feb 2020
TL;DR: Java chili extract ( Piper retrofractum Vahl.) was effective in decreased the number of leukocytes in W istar rats with traumatic ulcers.
Abstract: Background: Traumatic ulcer is an ulcerative lesion of the oral mucosa caused by trauma. The prevalence of traumatic ulcers is quite high between the other oral lesions. Handling traumatic ulcer use corticosteroids that have the effect of increase the growth of Candida sp. in the oral cavity (candidiasis) . Objective: To determine the effect of Java chili extract ( Piper retrofractum Vahl.) on leukocyte reduction in Wistar rats with traumatic ulcer. Methods: This is experimental laboratory study with pre and post test with a control group design. The research sample using 20 male Wistar rats, weight ±150 grams, 2-3 months old, divided into 4 treatment groups, (K1) group with untreated ulcers, (K2) rats with ulcers treated with iodine, (K3) rats with ulcers treated with java chili extract 50%, (K4) rats with ulcers treated with java chili extract 100%.The research was analyzed by different test with dependent sample t test . Results: The results showed a value of p <0.05 for all comparisons between the two groups showed that there were differences between the two groups compared have a significant difference in the number of leukocytes so it was known that Java chili extract decreased the number of leukocytes in W istar rats with traumatic ulcers. Conclusion: Java chili extract ( Piper retrofractum Vahl.) was effective in decreased the number of leukocytes in W istar rats with traumatic ulcers.
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TL;DR: Aqueous extracts of nine medicinal plants were bioassayed against larvae of Culex quinquefasciatus Say and Aedes aegypt (L.) and the long pepper, Piper retrofractum Vahl (Piperaceae), showed the highest level of activity against mosquito larvae.
Abstract: Aqueous extracts of nine medicinal plants were bioassayed against larvae of Culex quinquefasciatus Say and Aedes aegypt (L.). Among these plants, the long pepper, Piper retrofractum Vahl (Piperaceae), showed the highest level of activity against mosquito larvae. To gain more information on larvicidal activity of P. retrofractum, fresh fruits of this plant were extracted in water and the extracts made into powder and bioassayed against 3rd and 4th instar larvae of Cx. quinquefasciatus and Ae. aegypti in the laboratory. Extracts of unripe (001/3) and ripe (002/3 and 001/4) fruits showed different levels of activity against Cx. quinquefasciatus larvae. Extracts 001/3 and 002/3 were equi-toxic to a Bacillus sphaericus resistant and susceptible strains, both from Thailand. The ripe fruit extract 002/3 was somewhat more active against Ae. aegypti than Cx. quinquefasciatus. Another ripe fruit extract (001/4) was much more toxic to both mosquito species. Diluted solutions of the solid extract (002/3) in distilled water lost their larvicidal activity upon aging. Loss of activity at 25 degrees C was greater than that stored at 4 degrees C, and greater in water than in acetone solution.
94 citations
"Isolation of Piperin from The Fruit..." refers background in this paper
...A literature survey disclosed that a number biological studies have been carried out on this plant extract such as antioxidant, anti-fungal, cytotoxic and also shown the α-glucosidase inhibitory activity [2-8], and revealed that piperidine alkaloids, amides, and phenylpropanoid are the major secondary metabolites isolated....
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TL;DR: Three new amides, (2E,14Z)-N-isobutyleicosa-2,14-dienamide (1), dipiperamides F and G (2 and 3), together with 30 known compounds were isolated from the fruits of Piper retrofractum by extensive spectroscopic analyses and a plausible biosynthetic pathway leading to the amides isolated is discussed.
38 citations
"Isolation of Piperin from The Fruit..." refers background in this paper
...A literature survey disclosed that a number biological studies have been carried out on this plant extract such as antioxidant, anti-fungal, cytotoxic and also shown the α-glucosidase inhibitory activity [2-8], and revealed that piperidine alkaloids, amides, and phenylpropanoid are the major secondary metabolites isolated....
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TL;DR: Two new piperidine alkaloids, piperoctadecalidine and pipereicosalidine have been isolated from the fruits of Piper retrofractum (Piperaceae) along with two known piperine and pipernonaline as discussed by the authors.
33 citations
"Isolation of Piperin from The Fruit..." refers background in this paper
...A literature survey disclosed that a number biological studies have been carried out on this plant extract such as antioxidant, anti-fungal, cytotoxic and also shown the α-glucosidase inhibitory activity [2-8], and revealed that piperidine alkaloids, amides, and phenylpropanoid are the major secondary metabolites isolated....
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TL;DR: Two new compounds, piperoside and isoheptanol 2(S)-O-β-D-xylopyranosyl (1→6)-O -β- D-glucopyranuside (11) are identified and their structures are determined from 1D-NMR, 2D- NMR, and HR-ESI-MS spectral, a modified Mosher's method, and comparisons with previous reports.
31 citations
"Isolation of Piperin from The Fruit..." refers background in this paper
...A literature survey disclosed that a number biological studies have been carried out on this plant extract such as antioxidant, anti-fungal, cytotoxic and also shown the α-glucosidase inhibitory activity [2-8], and revealed that piperidine alkaloids, amides, and phenylpropanoid are the major secondary metabolites isolated....
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