Journal ArticleDOI
Isolation of putative progenitor endothelial cells for angiogenesis.
Takayuki Asahara,Toyoaki Murohara,Alison Sullivan,Marcy Silver,Rien van der Zee,Tong Li,Bernhard Witzenbichler,Gina C. Schatteman,Jeffrey M. Isner +8 more
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TLDR
It is suggested that EC progenitors may be useful for augmenting collateral vessel growth to ischemic tissues (therapeutic angiogenesis) and for delivering anti- or pro-angiogenic agents, respectively, to sites of pathologic or utilitarianAngiogenesis.Abstract:
Putative endothelial cell (EC) progenitors or angioblasts were isolated from human peripheral blood by magnetic bead selection on the basis of cell surface antigen expression In vitro, these cells differentiated into ECs In animal models of ischemia, heterologous, homologous, and autologous EC progenitors incorporated into sites of active angiogenesis These findings suggest that EC progenitors may be useful for augmenting collateral vessel growth to ischemic tissues (therapeutic angiogenesis) and for delivering anti- or pro-angiogenic agents, respectively, to sites of pathologic or utilitarian angiogenesisread more
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Vascular endothelial growth factor: basic science and clinical progress.
TL;DR: Vascular endothelial growth factor (VEGF) is an endothelial cell-specific mitogen in vitro and an angiogenic inducer in a variety of in vivo models and is implicated in intraocular neovascularization associated with diabetic retinopathy and age-related macular degeneration.
Journal ArticleDOI
Stromal Fibroblasts Present in Invasive Human Breast Carcinomas Promote Tumor Growth and Angiogenesis through Elevated SDF-1/CXCL12 Secretion
Akira Orimo,Piyush Gupta,Dennis C. Sgroi,Fernando Arenzana-Seisdedos,Thierry Delaunay,Rizwan Naeem,Vincent J. Carey,Andrea L. Richardson,Robert A. Weinberg +8 more
TL;DR: Using a coimplantation tumor xenograft model, it is demonstrated that carcinoma-associated fibroblasts extracted from human breast carcinomas promote the growth of admixed breast carcinoma cells significantly more than do normal mammaries derived from the same patients.
Journal ArticleDOI
Bone Marrow Origin of Endothelial Progenitor Cells Responsible for Postnatal Vasculogenesis in Physiological and Pathological Neovascularization
Takayuki Asahara,Haruchika Masuda,Tomono Takahashi,Christoph Kalka,Christopher Pastore,Marcy Silver,Marianne Kearne,Meredith Magner,Jeffrey M. Isner +8 more
TL;DR: Findings indicate that postnatal neovascularization does not rely exclusively on sprouting from preexisting blood vessels (angiogenesis); instead, EPCs circulate from bone marrow to incorporate into and thus contribute to postnatal physiological and pathological neov vascularization, which is consistent with postnatal vasculogenesis.
Journal ArticleDOI
Circulating Endothelial Progenitor Cells, Vascular Function, and Cardiovascular Risk
Jonathan Hill,Gloria Zalos,Julian Halcox,William H. Schenke,Myron A. Waclawiw,Arshed A. Quyyumi,Toren Finkel +6 more
TL;DR: A strong correlation between the number of circulating endothelial progenitor cells and the subjects' combined Framingham risk factor score was observed and measurement of flow-mediated brachial-artery reactivity revealed a signifi...
Journal ArticleDOI
Role of the Vascular Endothelial Growth Factor Pathway in Tumor Growth and Angiogenesis
Daniel J. Hicklin,Lee M. Ellis +1 more
TL;DR: Recently, an anti-VEGF antibody (bevacizumab), when used in combination with chemotherapy, was shown to significantly improve survival and response rates in patients with metastatic colorectal cancer and thus, validate VEGF pathway inhibitors as an important new treatment modality in cancer therapy.
References
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Failure of blood-island formation and vasculogenesis in Flk-1-deficient mice.
Fouad Shalaby,Janet Rossant,Janet Rossant,Terry P. Yamaguchi,Terry P. Yamaguchi,Marina Gertsenstein,Xiang-Fu Wu,Xiang-Fu Wu,Martin L. Breitman,Martin L. Breitman,Andre C. Schuh +10 more
TL;DR: The generation of mice deficient in Flk-1 by disruption of the gene using homologous recombination in embryonic stem (ES) cells is reported, indicating that FlK-1 is essential for yolk-sac blood-island formation and vasculogenesis in the mouse embryo.
Journal ArticleDOI
The fms-Like Tyrosine Kinase, a Receptor for Vascular Endothelial Growth Factor
TL;DR: Findings show that flt encodes a receptor for VEGF-VPF, a factor that induces vascular permeability when injected in the guinea pig skin and stimulates endothelial cell proliferation.
Journal ArticleDOI
High affinity VEGF binding and developmental expression suggest Flk-1 as a major regulator of vasculogenesis and angiogenesis.
Birgit Millauer,Susanne Wizigmann-Voos,Harald Schnürch,Ricardo Martinez,Niels Møller,Werner Risau,Axel Ullrich +6 more
TL;DR: Investigation of flk-1 receptor tyrosine kinase mRNA expression by in situ hybridization analysis revealed specific association with endothelial cells at all stages of mouse development, suggesting a major role of this ligand-receptor signaling system in vasculogenesis and angiogenesis.
Journal ArticleDOI
Distinct roles of the receptor tyrosine kinases Tie-1 and Tie-2 in blood vessel formation
Thomas N. Sato,Yuzuru Tozawa,Urban Deutsch,Karen Wolburg-Buchholz,Yuko Fujiwara,Maureen Gendron-Maguire,Thomas Gridley,Hartwig Wolburg,Werner Risau,Ying Qin +9 more
TL;DR: In vivo analyses of embryos deficient in Tie-2 showed that it is important in angiogen-esis, particularly for vascular network formation in endothelial cells, which contrasts with previous reports on Tie-1 function in vasculogenesis and/or endothelial cell survival.
Journal ArticleDOI
Identification of the KDR tyrosine kinase as a receptor for vascular endothelial cell growth factor
Bruce I. Terman,Maureen Dougher-Vermazen,Miguel Eduardo Carrion,Dragan Dimitrov,Douglas C. Armellino,Denis Gospodarowicz,Peter Bohlen +6 more
TL;DR: The KDR receptor tyrosine kinase shares structural similarities with a recently reported receptor for VEGF, flt, in a manner reminiscent of the similarities between the alpha and beta forms of the PDGF receptors.