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Journal ArticleDOI

Karyotype diversity and interspecific 4C DNA variation inBupleurum

01 Sep 1993-Biologia Plantarum (Springer Netherlands)-Vol. 35, Iss: 3, pp 355-363
TL;DR: Investigation on karyotype, 4C nuclear DNA amount and interphase nuclear volume (INV) of different HimalayanBupleurum species belonging to Umbelliferae revealed genetic differentiation.
Abstract: Investigation on karyotype, 4C nuclear DNA amount and interphase nuclear volume (INV) of different HimalayanBupleurum species belonging toUmbelliferae revealed genetic differentiation. Numerical and structural alternation of chromosomes in interspecific level were manifested in their statistically significant altered species specific 4C nuclear DNA content. Somatic chromosome number ranged between 2n = 14 and 2n = 16.B. himalayense was reported for the first time having 2n = 16 chromosomes. Correlation coefficient among the various chromosomal and nuclear parameters showed no significant progressive or regressive interdependence except in between INV and nuclear DNA amount. Critical differences between 4C DNA content showed interspecific variation.
Topics: Nuclear DNA (59%), Karyotype (55%), Chromosome (54%)
Citations
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Journal ArticleDOI
01 Aug 1997-Annals of Botany
TL;DR: A supplementary list of nuclear DNA C-values from 37 sources for 471 angiosperm species not listed in the aforementioned compilations, plus additional estimates for 113 species already listed by them are presented, which significantly improves representation of the global flora.
Abstract: The amount of DNA in the unreplicated haploid nuclear genome (its C-value) varies over 600-fold between angiosperm species. Information regarding this character is used in a strikingly wide variety of plant biological fields. Moreover, recent studies have noted a significant need for more information about this important aspect of genome biodiversity. Bennett and co-authors have published four collected lists of nuclear DNA amounts in angiosperm species, compiled primarily for reference purposes, including the most recent in 1995 (Annals of Botany 76: 113‐176). Together they list estimates for over 2500 species which represent about 1% of the global angiosperm flora. Interest in angiosperm genome size has remained high, as shown by the recent publication of many new estimates, creating a need for a fifth compilation. This paper presents a supplementary list of nuclear DNA C-values from 37 sources for 471 angiosperm species not listed in the aforementioned compilations, plus additional estimates for 113 species already listed by them. It contains estimates for palm, orchid and tropical hardwood species which significantly improves representation of the global flora. Work is in hand to combine the genome size data compiled in this and the aforementioned papers into a unified database, and to present the information in separate lists, with species in alphabetical and systematic orders, respectively. Meanwhile, the availability of DNA C-values for angiosperm species can be checked on the World Wide Web (http:}}www.rbgkew.org.uk}cval}database1.html). # 1997 Annals of Botany Company

186 citations


Journal ArticleDOI
TL;DR: Evidence from morphology, crossability, pollen viability and chromosome synapsis suggests a segmental allopolyploid origin for M. cymbalaria, a taxon of controversial taxonomic identity of the Momordica genus.
Abstract: Somatic chromosome number and detailed karyotype analysis were carried out in six Indian Momordica species viz. M. balsamina, M. charantia, M. cochinchinensis, M. dioica, M. sahyadrica and M. cymbalaria (syn. Luffa cymbalaria; a taxon of controversial taxonomic identity). The somatic chromosome number 2n = 22 was reconfirmed in monoecious species (M. balsamina and M. charantia). Out of four dioecious species, the chromosome number was reconfirmed in M. cochinchinensis (2n = 28), M. dioica (2n = 28) and M. subangulata subsp. renigera (2n = 56), while in M. sahyadrica (2n = 28) somatic chromosome number was reported for the first time. A new chromosome number of 2n = 18 was reported in M. cymbalaria against its previous reports of 2n = 16, 22. The karyotype analysis of all the species revealed significant numerical and structural variations of chromosomes. It was possible to distinguish chromosomes of M. cymbalaria from other Momordica species and also between monoecious and dioecious taxa of the genus. Morphology and crossability among the dioecious species was also studied. Evidence from morphology, crossability, pollen viability and chromosome synapsis suggests a segmental allopolyploid origin for M. subangulata subsp. renigera. The taxonomic status of the controversial taxon M. cymbalaria was also discussed using morphological, karyological and crossability data.

47 citations


Journal ArticleDOI
25 Jun 1997-Cytologia
TL;DR: The structural alteration of chromosomes as well as loss or addition of highly repetitive sequences in the genome showed variation in the DNA amount at cultivar level, but a marginal variation in nuclear DNA content at the cultivarlevel indicated a close relationship between them.
Abstract: Extensive karyotype analysis including determination of somatic chromosome number, total chromosome length and volume and estimation of 4C DNA amount were carried out on 9 different cultivars of ginger (Zingiber officinale Rose.) for the first time. A significant variation in nuclear DNA amount was recorded at the cultivar level. The 4C DNA amount varied from 19.663-24.102 pg in the cultivars studied. The correlation coefficient studies showed that the 4C DNA content and genomic chromosome volume were interdependent. The structural alteration of chromosomes as well as loss or addition of highly repetitive sequences in the genome showed variation in the DNA amount at cultivar level, but a marginal variation in nuclear DNA content at the cultivar level indicated a close relationship between them.

27 citations


Journal ArticleDOI
01 Nov 2004-Genetica
TL;DR: By employing RAPD markers, karyotypes and 4C DNA content analysis, the present study has helped to resolve the relationship between the taxonomically diverse leguminous mangroves and study their ability to coexist with mangrove that would shed light on the evolution ofMangroves from terrestrial species.
Abstract: Randomly amplified polymorphic DNA (RAPD) markers, karyotypes and 4C DNA content were analyzed in five legume mangroves belonging to the sub-family Papilinoideae (Dalbergia spinosa, Derris heterophylla and D. indica) and Caesalpinioideae (Caesalpinia crista, Cynometra ramiflora) of the family Fabaceae to establish the genetic variability and phylogenetic affinities. Somatic chromosome numbers were reported for the first time in D. spinosa (2n = 20), C. ramiflora (2n = 26) and D. heterophylla (2n = 24) with reconfirmation of the somatic chromosome number in D. indica (2n = 22) and C. crista (2n = 24). Significant intergeneric and interspecific variation of 4C DNA content was observed and that varied from 8.970 pg in C. ramiflora to 28.730 pg in D. indica. From the RAPD analysis, the dendogram showed clustering of Caesalpinia crista and Cynomitra ramiflora into one group (81.80). In the second groups Derris indica and Derris heterophylla were more similar (83.10) than Dalbergia spinosa (85.80). Species-specific DNA markers (900 bp) obtained in D. spinosa from OPN15; 700 and 2000 bp in C. ramiflora from OPN4 and 400 and 800 bp in D. heterophylla and 500 bp DNA fragment in C. crista obtained from OPN-11 were found characteristic RAPD markers of these species. C. crista found more closer affinity to C. ramiflora of the sub-family Caesalpinioideae [genetic distance (1-F) = 0.847]. Derris indica showed closer genetic relation with D. heterophylla [genetic distance (1-F) = 0.856] than D. spinosa [genetic distance (1-F) = 0.876] where Derris and Dalbergia belongs to the sub-family Papilionoideae. By employing these markers the present study has helped to resolve the relationship between the taxonomically diverse leguminous mangroves and study their ability to coexist with mangroves that would shed light on the evolution of mangroves from terrestrial species.

20 citations


Journal ArticleDOI
TL;DR: Random amplified polymorphic DNA (RAPD) markers are used to estimate interspecific variation among mangrove and non-mangrove Heritiera fomes, H. littoralis and H. macrophylla to find characteristic molecular markers in H. Macrophylla, a species from a non-reproductive habitat.
Abstract: Random amplified polymorphic DNA (RAPD) markers are used to estimate interspecific variation among mangrove and non-mangrove Heritiera fomes, H. littoralis and H. macrophylla. All the species have 2n =38 chromosomes, with minute structural changes distinguishing the karyotype of each species. Significant variation of 4C DNA content occurs at the interspecific level. Interspecific polymorphism ranged from 14.09% between H. fomes and H. littoralis to 52.73% between H. fomes and H. macrophylla. H. macrophylla showed wide polymorphism in the RAPD marker with H. littoralis (51.23%) and H. fomes (52.73%) . Two distinct RAPD products obtained from OPA-10 (1000 bp) and OPD-15 (900 bp) found characteristic molecular markers in H. macrophylla, a species from a non-mangrove habitat. H. macrophylla was more distantly related to H. fomes[genetic distance (1-F)=0.305] than to H. littoralis[genetic distance (1-F)=0.273]. H. littoralis was of a closer affinity to H. fomes[genetic distance (1-F)=0.218] than to H. macrophylla.

17 citations


References
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Journal ArticleDOI
02 Sep 2009-Hereditas
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