Journal ArticleDOI
Karyotype stability in long-term callus derived plants ofCrepis tectorum L.
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TLDR
The study of in vitro growth of Crepis tectorum revealed 100 % callusing and 40 % plantlet regeneration and the leaf-tips of regenerated plants were homogeneous and identical to the donor plant both in number and morphology of chromosomes.Abstract:
The study of in vitro growth of Crepis tectorum revealed 100 % callusing and 40 % plantlet regeneration. The root and leaf used as explants showed the normal diploid (2n=8) chromosome constitution. In one month old culture 95 % callus cells were diploid. The callus maintained in 2,4-D 1 mg 1-1 for two years showed 62 % diploid, 5 % tetraploid and 33 % hyperdiploid cells. The differentiation of shoot occurred in two year old calli after subeulturing in 2 mg I-1 BAP and the potentiality of regeneration was retained for more than one year. The leaf-tips of regenerated plants were homogeneous and identical to the donor plant both in number and morphology of chromosomes.read more
Citations
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Journal ArticleDOI
Nuclear genome stability in long-term cultivated callus lines of Fagopyrum tataricum (L.) Gaertn.
Alexander Betekhtin,Magdalena Rojek,Joanna Jaskowiak,Anna Milewska-Hendel,Jolanta Kwasniewska,Yulia Kostyukova,Ewa Kurczyńska,N. I. Rumyantseva,N. I. Rumyantseva,Robert Hasterok +9 more
TL;DR: In this paper, the authors used histological sections and transmission electron microscopy to identify and describe the morphology of the nuclei of all of the analysed callus lines and found that the embryogenic callus cells had prominent round nuclei that did not contain heterochromatin clumps in contrast to the non-morphogenic calluses lines, in which they found nuclei with multiple lobes.
Journal ArticleDOI
Increased miroestrol, deoxymiroestrol and isoflavonoid accumulation in callus and cell suspension cultures of Pueraria candollei var. mirifica
TL;DR: This is the first report to suggest that callus culture is a suitable alternative method for producing miroestrol and deoxymirostrol in in vitro cell culture of P. mirifica.
Journal ArticleDOI
Characterization of the Variability of Nucleoli in the Cells of Panax ginseng Meyer In Vivo and In Vitro.
Yuliya A. Khrolenko,O. L. Burundukova,Lyudmila S. Lauve,T. I. Muzarok,Vyacheslav V. Makhan’kov,Yuri N. Zhuravlev +5 more
TL;DR: It was shown that long-time cells (cultivated since 1988) had cytogenetic changes such as increase level of polyploid and aneuploid cells, increase of nucleoli number into interphase nucleus and decrease of nuclei/nucleoli ratio.
Book ChapterDOI
Morphogenesis, Genetic Stability, and Secondary Metabolite Production in Untransformed and Transformed Cultures
Mihir Halder,Sumita Jha +1 more
TL;DR: This review attempts to highlight an overall knowledge about the role of differentiation and morphogenesis on SM production with genetic and biochemical stability of commonly used different in vitro cultures.
References
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Journal ArticleDOI
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more
TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
Journal ArticleDOI
Morphogenesis in Relation to Chromosomal Constitution in Long-term Plant Tissue Cultures
TL;DR: It was found that during the prolonged period of subculture there was a progressive loss of organ-forming Capacity in all tissue strains, paralleled by increasing abnormalities in the chromosomal constitution, including higher chromosome numbers and greater frequency of aneuploidy.
Journal ArticleDOI
Karyotypic changes in callus cultures from haploid and diploid plants of Crepis capillaris (L.) Wallr
TL;DR: Two auxin-heterotrophic callus cultures of Crepis capillaris were studied and it has been found that new karyotypes also originate through chromosome rearrangements at the same ploidy level as the original explant.
Book ChapterDOI
Plant Regeneration and Chromosome Stability in Tissue Cultures
TL;DR: Since the initial reports in 1939 independently of the successful growth and subculturing of tissue cultures of carrot and tobacco, these two dicots and a multitude of others, including soybeans, bush beans, tomato, potato, belladonna, sycamore and Jerusalem artichoke have been extensively cultured and used for a variety of studies.
Journal ArticleDOI
Karyological and Cytophotometric Study of Callus Induction in Allium sativum L.
Jaroslav Doležel,F. J. Novák +1 more
TL;DR: Callus induction was accompanied by an increase in the ploidy level anditution mitoses were most probably the main mechanism of origin of polyploid cells.
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