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All figures (11)
FIG. 4. Effect of varying exposure time of plasma treatment on the viability of CCRF-CEM cells. The cell viability was determined using trypan blue exclusion assays, and the values are expressed as the mean percentage of total viable cells 6 standard deviation of three separate experiments. (a) Cell viability determined at 12 h postplasma treatment and (b) cell viability determined at 36 h postplasma treatment.
FIG. 5. Photograph of the trypan blue exclusion assay showing the live vs dead cells after 3 min of exposure to plasma treatment sampled at 12 h posttreatment. A ratio of 1:1 of the trypan blue dye (0.4% concentration) to 106 cells in media was used and the mixture was loaded on a hemocytometer. The cells that are dead absorb the trypan blue dye while live cells do not. The difference is clearly observed using a bright field microscope at a magnification of 25 .
FIG. 6. Percent viability vs exposure time for cancer cells. Data taken 48 h postexposure.
FIG. 8. Image of untreated SCaBER cancer cells grown in MEM complete growth media at a magnification of 10 using an inverted bright field microscope.
FIG. 7. Morphology of DU 145 cancer cells, control (a) and plasma treated for 10 min (b).
FIG. 9. Image of SCaBER cells after the trypan blue exclusion assay showing cells treated by LTP for 2 min at 12 h postplasma exposure. Live cells are bright/whitish (indicated by the circle) as the dye cannot penetrate into the intact cell membrane while dead cells are stained blue/dark (indicated by the arrow). The image is at 10 magnification using a bright field microscope.
FIG. 11. Images of SCaBER cells reattaching to the surface of the culture plate at different times after reseeding. Photos were taken at specific times as noted on each image. Panel A is the control SCaBER cells that were not treated. The untreated cells adhere and are responsive at 1.5 h post-reseeding. Panel B is a 2 min LTP treatment and panel C is a 5 min LTP treatment. The earliest reattachment time post-reseeding for the 5 min LTP treatment was at 4.5 h as indicated by a few cells beginning to adhere to the surface.
FIG. 10. Cell viability of SCaBER cells treated in media reveal dead (dark bars on top) and live (gray bars below). The viability was monitored for 0, 12, 24, and 48 h post-LTP exposure.
FIG. 1. Schematic of the plasma pencil.
FIG. 3. Experimental setup for the exposure of samples to the plasma pencil.
FIG. 2. Cold plasma plume touching the author’s hand.
Journal Article
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DOI
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Killing adherent and nonadherent cancer cells with the plasma pencil
[...]
Mounir Laroussi
,
Soheila Mohades
,
Nazir Barekzi
08 Jan 2015
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