Journal ArticleDOI
l-Phenylalanine ammonia-lyase: IV. Evidence that the prosthetic group contains a dehydroalanyl residue and mechanism of action
Reads0
Chats0
TLDR
It is proposed that the active site contains a dehydroalanyl residue which upon reduction yields a tritiated alanyl residue so substituted at the nitrogen atom that HCl hydrolysis leads to racemization and tritium loss.About:
This article is published in Archives of Biochemistry and Biophysics.The article was published on 1970-11-01. It has received 133 citations till now. The article focuses on the topics: Active site & Phenylalanine.read more
Citations
More filters
Journal ArticleDOI
Phenylalanine ammonia lyase
Edith L. Camm,G.H.Neil Towers +1 more
TL;DR: The literature concerning the physiology and biochemistry of the enzyme phenylalanine ammonia lyase (PAL) (E.C. 4.1.1) from different organisms has been reviewed as mentioned in this paper.
Book ChapterDOI
Phytoalexins: enzymology and molecular biology.
Journal ArticleDOI
Phenylalanine ammonia-lyase - regulation of its induction, and its role in plant development
TL;DR: The properties of phenylalanine ammonia-lyase from higher plants and its position in phenylpropanoid metabolism are briefly reviewed.
Journal ArticleDOI
A modern view of phenylalanine ammonia lyase
TL;DR: Key details relating to the physiological role of PAL, the mechanism of catalysis, methods of determination and purification, enzyme kinetics, and enzyme activity in nonaqueous media are discussed.
Journal ArticleDOI
New Generation of Biocatalysts for Organic Synthesis
TL;DR: The advantages and applications of the most recent and attractive biocatalysts--reductases, transaminases, ammonia lyases, epoxide hydrolases, and dehalogenases--will be discussed herein and exemplified by the syntheses of interesting compounds.
References
More filters
Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Book ChapterDOI
[117] Chromatographic determination of amino acids by the use of automatic recording equipment
Stanford Moore,William H. Stein +1 more
TL;DR: This chapter describes the chromatographic determination of amino acids by the use of automatic recording equipments and suggests that air-oxidation of cysteine in the hydrolyzate to cystine, before chromatography, is desirable in order to obtain a more accurate value for the total cyStine + cySteine content of the protein.
Journal ArticleDOI
Amino acid analysis: aqueous dimethyl sulfoxide as solvent for the ninhydrin reaction.
TL;DR: Dimethyl sulfoxide can replace the latter, volume for volume, in a ninhydrin reagent mixture that gives equal performance and has improved stability, and is recommended to replace methyl Cellosolve-containing reagents in the quantitative determination of amino acids by automatic analyzers and by the manual ninHydrin method.