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Book ChapterDOI

Large Scale Preparation of Highly-Purified Egg Yolk Phosphatidylcholine by HPLC

TL;DR: The system which refines egg phosphatidylcholine (PC) for pharmaceutical and food use was developed and the purity of obtained PC was about 100%.
Abstract: We studied to develop the system which refines egg phosphatidylcholine (PC) for pharmaceutical and food use. HPLC was applied with silicagel particles(YMC-GEL S-50) as the column packing and ethanol/water(92:8) as the safety mobile phase. Six hundred grams of egg lecithin PL-100H (Q.P. Corp.) were separated into PC and phosphatidylethanolamine(PE) at a time. The purity of obtained PC was about 100%. And we could easily recover the mobile phase by distillation and use it repeatedly.
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Journal ArticleDOI
TL;DR: A convenient method for the separation of molecular species of phosphatidylcholine by reversed-phase high performance liquid chromatography (HPLC) is described, finding the average HCN to be remarkably the same for all the three different naturally occurring sources considering a wide variety of different fatty acid compositions of each type.

131 citations