Larvicidal activity of neem oil (Azadirachta indica) formulation against mosquitoes
TL;DR: The neem oil formulation was found effective in controlling mosquito larvae in different breeding sites under natural field conditions and may prove to be an effective and eco-friendly larvicide, which could be used as an alternative for malaria control.
Abstract: Background
Mosquitoes transmit serious human diseases, causing millions of deaths every year. Use of synthetic insecticides to control vector mosquitoes has caused physiological resistance and adverse environmental effects in addition to high operational cost. Insecticides of botanical origin have been reported as useful for control of mosquitoes. Azadirachta indica (Meliaceae) and its derived products have shown a variety of insecticidal properties. The present paper discusses the larvicidal activity of neem-based biopesticide for the control of mosquitoes.
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Patent•
20 Dec 2018
TL;DR: In this paper, the present invention is directed to pesticidal mixtures comprising sabadilla alkaloids and neem oil and methods of controlling pests including insects and mites.
Abstract: The present invention is directed to pesticidal mixtures comprising sabadilla alkaloids and neem oil and methods of controlling pests including insects and mites by application of pesticidal mixtures comprising sabadilla alkaloids and neem oil.
TL;DR: In this article , the effect of Zingiber officinale and Allium sativum extracts against filariasis vectors was evaluated by introducing 10 larvae of the vectors to the concentrations of 250, 500, and 750ppm of the crude sample, and data were subjected to probit analysis to determine the LC50 and Chi-squared test to check the significance of the mortality by R software.
Abstract: Mosquitoes present an immense threat to millions of people worldwide and act as vectors for filariasis disease. The objective of the study was to determine the effect of Allium sativum and Zingiber officinale extracts against filariasis vectors. The larvae were collected from the breeding site by using standard procedures for identification and larvicidal activities. Twenty grams (20 g) from each (Allium sativum and Zingiber officinale) were extracted separately by aqueous, ethanol, and methanol solvents. The phytochemical analysis was determined in the crude sample by using standard methods. Then, larvicidal effects were determined by introducing 10 larvae of the vectors to the concentrations of 250 ppm, 500 ppm, and 750 ppm of the crude sample, and data were subjected to probit analysis to determine the LC50 and Chi-squared test to check the significance of the mortality by R software. Anopheles funestus, Anopheles gambiae s.l., Anopheles pharoensis, Culex antennatus, and Culex quinquefasciatus were the filariasis vectors identified during the study period. The presence of phytochemical tests such as anthraquinones, flavonoids, glycosides, phenol, saponin, steroids, tannin, and terpenes was obtained. The larvicidal effects of the selected plant extracts ranged from 0%-100%. The lowest LC50 (53 ppm) was observed for A. sativum methanol test extract against Cx. quinquefasciatus. Ethanol extracts of A. sativum have a significant effect on An. funestus (X2 = 7.5, p = 0.02352) and Cx. quinquefasciatus (X2 = 10.833, p = 0.0.0044), whereas aqueous extracts have a significant effect only on An. gambiae s.l. (X2 = 7.0807, p = 0.029. Ethanol extracts of Z. officinale have a significant effect only on the mortality of An. pharoensis (X2 = 7.0807, p = 0.029), but methanol and aqueous extracts have no significant effect against filariasis vectors. In conclusion, A. sativum have a high toxic effect than Z. officinale extract against filariasis vectors in all type of solvents. So using those plant extracts is the best to reduce the risk of the synthetic chemical on nontarget organisms and the environment, in addition to the control of mosquito-borne diseases, but further studies will be conducted to evaluate the toxicity at different stages of the vectors.
TL;DR: In this article , the authors highlighted the application of biodiesel as a carrier fuel with azadirachta indica oil as insecticide in thermal fogging system towards adult mosquitoes and mosquito larvae.
Abstract: The study highlighted the application of biodiesel as a carrier fuel with azadirachta indica oil as insecticide in thermal fogging system towards adult mosquitoes and mosquito larvae. The open fogging test is conducted indoor inside a visible container (dimension: 50 cm x 50 cm x 50 cm) with different dilution ratio of azadirachta indica oil and biodiesel to determine the ideal ratio for the formulation to give effects towards mosquitoes and mosquito larvae effectively. Spread-ability test is also conducted to examine the effectiveness of the insecticide-carrier solution in a real-life simulation. The biodiesel used was produced through the hydrotalcite catalyzed transesterification derived from palm oil. The adulticidal and larvicidal activity of biodiesel and azadirachta indica oil as fuel-insecticide is evaluated through dilution ratio of 9:1, 1:1 and 3:7. The fuel-insecticide solution is dispersed in the visible tank by heating it to produce fog. From the tests conducted, the most ideal dilution ratio effect is 1:1 towards both adult mosquito and mosquito larvae. The ratio indicated the shortest time to achieve 100% mortality rate in adult mosquitoes (20 minutes). While in spread-ability test, 55 minutes was taken to achieve total mortality. In larvicidal potential, the ratio of 1:1 showed the highest larvae deceased which is 2 out of 3 larvae for both open and spread-ability test.
TL;DR: An overview of recent advances in research on the environmental impact of neem products and their use and effectiveness as a biopesticide is presented.
Abstract: Pesticides have both acute and chronic effects on humans and all other warm blooded living things. In pesticide applications, few of the amount used reaches the target organisms, whereas the rest mixes into the ecosystem and leads to the deterioration of the ongoing natural balance. Therefore, growing attention has been given toward the development of alternate environmentally friendly pesticides/insecticides that would aid an efficient pest management system and also prevent chronic exposures leading to diseases. In this context, plant-derived natural products are considered valuable candidates to reverse this negative trend. Botanical pesticides are relatively safe, degradable, and are readily available sources of biopesticides. Neem is an ideal alternative candidate as a natural non-synthetic plant pesticide. The neem products have been obtained from several species of neem trees in the family Meliaceae. Array of more than 300 bioactive compounds in the neem tree makes it a unique plant with potential applications in pest and vector management. Botanical pesticides, such as neem, have limited persistence in the environment, and ultraviolet light, temperature, rainfall and other environmental factors can degrade neem. Unlike toxic synthetic insecticides, neem materials do not kill the pest, but incapacitate or neutralize it via cumulative behavioural, physiological, and cytological effects. In spite of high selectivity, neem materials affect more than 400 harmful species including insect pests, phytophagous mites, mites, and ticks affecting man and animals, parasitic protozoans, noxious molluscs, plant parasitic nematodes, pathogenic fungi, and harmful bacteria and fungi. Neem ingredients affect insects in various ways such as repellent, antifeedant, toxic, fecundity and growth regulatory effects. This review presents an overview of recent advances in research on the environmental impact of neem products and their use and effectiveness as a biopesticide.
TL;DR: The study provided that margosa oil is promising as a larvicidal agent against Cx.
Abstract: in the gizzard dendrites. The study provided that margosa oil is promising as a larvicidal agent against Cx. pipiens , naturally revolved biopesticides would be an alternative to chemical insecticides.
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TL;DR: In order to make experimental studies comparable and statistically meaningful, the article recommends the following formula: per cent control = 100(X - Y)/X, which eliminates errors due to deaths in the control sample which were not due to the insecticide.
Abstract: There are several statistical methods used in biology (entomology) for computing the effectiveness of an insecticide, based on relating the number of dead insects in the treated plat to the number of live ones in the untreated plat. In order to make experimental studies comparable and statistically meaningful, the article recommends the following formula: per cent control = 100(X - Y)/X, where X = % living in the untreated check sample and Y = % living in the treated sample. Calculation using this method eliminates errors due to deaths in the control sample which were not due to the insecticide. An example based on treatments of San Jose scale includes computation of probable errors for X and Y, and the significance of the difference between the two counts. Common biometric convention holds that when the difference between the results of two experiments is greater than three times its probable error, the results are significant and due to the treatment applied.
11,700 citations
"Larvicidal activity of neem oil (Az..." refers methods in this paper
...The percent corrected mortality was calculated using Abbott's formula [13] and Log probit analysis was used to determine the median lethal concentration (LC50)/90% lethal concentration (LC90) of the formulation....
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TL;DR: In the context of agricultural pest management, botanical insecticides are best suited for use in organic food production in industrialized countries but can play a much greater role in the production and postharvest protection of food in developing countries.
Abstract: Botanical insecticides have long been touted as attractive alternatives to synthetic chemical insecticides for pest management because botanicals reputedly pose little threat to the environment or to human health. The body of scientific literature documenting bioactivity of plant derivatives to arthropod pests continues to expand, yet only a handful of botanicals are currently used in agriculture in the industrialized world, and there are few prospects for commercial development of new botanical products. Pyrethrum and neem are well established commercially, pesticides based on plant essential oils have recently entered the marketplace, and the use of rotenone appears to be waning. A number of plant substances have been considered for use as insect antifeedants or repellents, but apart from some natural mosquito repellents, little commercial success has ensued for plant substances that modify arthropod behavior. Several factors appear to limit the success of botanicals, most notably regulatory barriers and the availability of competing products (newer synthetics, fermentation products, microbials) that are cost-effective and relatively safe compared with their predecessors. In the context of agricultural pest management, botanical insecticides are best suited for use in organic food production in industrialized countries but can play a much greater role in the production and postharvest protection of food in developing countries.
2,996 citations
"Larvicidal activity of neem oil (Az..." refers background in this paper
...Many of these derived products have antifeedancy, ovicidal activity, fecundity suppression besides insect growth regulation and repellency against insects [ 5-10 ]....
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TL;DR: Les substances derivees du «neem», efficaces contre les insectes sont examines (influence sur le comportement de fixation, the ponte, the prise de nourriture, the metamorphose, the fecondite, the fitners) ainsi que les capacites potentielles des insecticides extraits du neem pour le controle des insectes nuisibles and leurs effets sur les animaux.
Abstract: Les substances derivees du «neem», efficaces contre les insectes sont examines (influence sur le comportement de fixation, la ponte, la prise de nourriture, la metamorphose, la fecondite, la fitners) ainsi que les capacites potentielles des insecticides extraits du neem pour le controle des insectes nuisibles et leurs effets sur les animaux a sang chaud et sur l'homme
1,595 citations
Journal Article•
TL;DR: Examples of phytochemicals evaluated against mosquitoes as general toxicants, growth and reproduction inhibitors, repellents and ovipositional deterrents are given.
Abstract: A review on the reported uses of chemicals derived from botanical sources is presented, along with the part of the plant used for extraction, the mosquito species studied and the bioactivity observed for 344 plant species. Examples of phytochemicals evaluated against mosquitoes as general toxicants, growth and reproduction inhibitors, repellents and ovipositional deterrents are given. The effects of mosquito species and life stage specificity, solvents used for extraction, phototoxic activity and the geographical source from where the plant compounds are derived are discussed.
735 citations
Book•
01 Jan 1995
TL;DR: The tree and its characteristics biologically active ingredients effects on viruses and organisms neem products for pest management and practical results of neem applications against arthropod pests, and probability of development of resistance toxicity of neems to vertebrates and side effects on beneficial and other non-target organisms as discussed by the authors.
Abstract: The tree and its characteristics biologically active ingredients effects on viruses and organisms neem products for pest management and practical results of neem applications against arthropod pests, and probability of development of resistance toxicity of neem to vertebrates and side effects on beneficial and other non-target organisms various uses of neem products economic, socioeconomic and policy considerations, and neem in sociocultural life in South Asia other meliaceous plants containing ingredients for pest management and other purposes register of scientific and common names. (Part contents).
414 citations