Lateral flow assays (LFA) as an alternative medical diagnosis method for detection of virus species: The intertwine of nanotechnology with sensing strategies.
Poorya Sadeghi,Hessamaddin Sohrabi,Maryam Hejazi,Ali Jahanban-Esfahlan,Behzad Baradaran,Maryam Tohidast,Mir Reza Majidi,Ahad Mokhtarzadeh,Seyed Mohammad Tavangar,Miguel de la Guardia +9 more
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TLDR
In this paper, a review of point-of-care-testing (POCT) analysis is presented, where the authors discuss the advantages and limitations of the conventional and clinical approaches.Abstract:
Viruses are responsible for multiple infections in humans that impose huge health burdens on individuals and populations worldwide. Therefore, numerous diagnostic methods and strategies have been developed for prevention, management, and decreasing the burden of viral diseases, each having its advantages and limitations. Viral infections are commonly detected using serological and nucleic acid-based methods. However, these conventional and clinical approaches have some limitations that can be resolved by implementing other detector devices. Therefore, the search for sensitive, selective, portable, and costless approaches as efficient alternative clinical methods for point of care testing (POCT) analysis has gained much attention in recent years. POCT is one of the ultimate goals in virus detection, and thus, the tests need to be rapid, specific, sensitive, accessible, and user-friendly. In this review, after a brief overview of viruses and their characteristics, the conventional viral detection methods, the clinical approaches, and their advantages and shortcomings are firstly explained. Then, LFA systems working principles, benefits, classification are discussed. Furthermore, the studies regarding designing and employing LFAs in diagnosing different types of viruses, especially SARS-CoV-2 as a main concern worldwide and innovations in the LFAs' approaches and designs, are comprehensively discussed here. Furthermore, several strategies addressed in some studies for overcoming LFA limitations like low sensitivity are reviewed. Numerous techniques are adopted to increase sensitivity and perform quantitative detection. Employing several visualization methods, using different labeling reporters, integrating LFAs with other detection methods to benefit from both LFA and the integrated detection device advantages, and designing unique membranes to increase reagent reactivity, are some of the approaches that are highlighted.read more
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Lateral flow assays (LFA) for detection of pathogenic bacteria: A small point-of-care platform for diagnosis of human infectious diseases.
Hessamaddin Sohrabi,Mir Reza Majidi,Mina Fakhraei,Ali Jahanban-Esfahlan,Maryam Hejazi,Fatemeh Oroojalian,Behzad Baradaran,Maryam Tohidast,Miguel de la Guardia,Ahad Mokhtarzadeh +9 more
TL;DR: In this article , the main principles of lateral flow assays (LFAs), challenges, and prospects for more development in this field in sensing pathogenic bacteria have been summarized, and visually-read LFAs improvement to further progressive platforms have been explored by considering the prospects of this very flexible detection of pathogenic organisms.
Journal ArticleDOI
State of the art: Lateral flow assays toward the point-of-care foodborne pathogenic bacteria detection in food samples.
Hessamaddin Sohrabi,Mir Reza Majidi,P. Khaki,Ali Jahanban-Esfahlan,Miguel de la Guardia,Ahad Mokhtarzadeh +5 more
TL;DR: The present review has stressed on latest developments in LFAs-based platforms to detect various foodborne pathogenic bacteria such as Salmonella, Listeria, Escherichia coli, Brucella, Shigella, Staphylococcus aureus, Clostridium botulinum, and Vibrio cholera.
Journal ArticleDOI
Aptamer-Based Lateral Flow Assays: Current Trends in Clinical Diagnostic Rapid Tests
TL;DR: The applications of aptamer technology in LFAs in clinical diagnostic rapid tests for the detection of biomarkers, microbial analytes, hormones and antibiotics, and performance, advantages and drawbacks are discussed.
Journal ArticleDOI
State-of-the-art Cancer Biomarker Detection by Portable (Bio) Sensing Technology: A Critical Review
Hessamaddin Sohrabi,Nadia Bolandi,Afsaneh Hemmati,Shirin Eyvazi,Shahin Ghasemzadeh,Behzad Baradaran,Fatemeh Oroojalian,Mir Reza Majidi,Miguel de la Guardia,Ahad Mokhtarzadeh +9 more
TL;DR: In this article , the authors discuss the recent progress of mHealth bio-affinity technology from laboratory testing to mobile POCT tools and electronic portable assays for the detection of cancer biomarkers.
Journal ArticleDOI
Current and Perspective Sensing Methods for Monkeypox Virus
Ijaz Gul,Changyue Liu,Xi Yuan,Zhicheng Du,Shiyao Zhai,Zhen-lin Lei,Qun Chen,Muhammad Akmal Raheem,Qian He,Qiuyue Hu,Chufan Xiao,Zhang HaiHui,Runming Wang,Sanyang Han,Ke Du,Dongmei Yu,Canyang Zhang,Peiwu Qin +17 more
TL;DR: This article reviews the traditional and emerging nucleic acid detection approaches, immunodiagnostics, whole-particle detection, and imaging-based MPXV detection techniques, and provides recommended solutions to the problems.
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