Leaf rolling and stem fasciation in grass pea (Lathyrus sativus L.) mutant are mediated through glutathione-dependent cellular and metabolic changes and associated with a metabolic diversion through cysteine during phenotypic reversal.
Cites background from "Leaf rolling and stem fasciation in..."
...Generally, leaf rolling is largely regulated by several factors: hormones (Fujino et al. 2008; Lee et al. 2006), metabolic changes (Talukdar and Talukdar, 2014) and formation of specific cells, such as bulliform cell (Fang et al. 2012; Xiang et al. 2012)....
Cites background or methods or result from "Leaf rolling and stem fasciation in..."
...In agreement with the present findings, BSO-treated rlfL-1 mutant of Lathyrus induced Cys desulfuration which played prominent roles in reversal of cell proliferations and restoration of normal mitosis during low GSH level (Talukdar and Talukdar 2014b)....
...The result pointed out that cytosolic isoforms (Cu/Zn SODs) in both bean genotypes played pivotal roles in maintaining SOD activity during As-exposures, which confirmed earlier findings (Abercrombie et al. 2008; Talukdar and Talukdar 2013, 2014a)....
...Mutants deficient in Cys-desulfuration pathway exhibited overaccumulation of free Cys which led to excess ROS generation and oxidative imbalance (Álvarez et al. 2010; Talukdar 2014) but relieved when Cys-desulfuration was induced (Talukdar and Talukdar 2014b)....
...Inverse relationship between GSH pool and expressions of GO transcripts was also observed in rlfL-1 mutant of grass pea in which diminishing GSH redox due to BSO treatment led to enhanced GO expressions and photorespiratory H2O2 production (Talukdar and Talukdar 2014b)....
...Relative gene expression analysis through quantitative RT-PCR Total RNA isolation and first-strand cDNA synthesis were done following manufacturer’s (Chromous Biotech, Bangalore, India) instructions, as detailed earlier (Talukdar and Talukdar 2014a)....
"Leaf rolling and stem fasciation in..." refers methods in this paper
...APX activity was expressed as μmol ascorbate oxidized minmg protein ....