Light and the E3 ubiquitin ligase COP1/SPA control the protein stability of the MYB transcription factors PAP1 and PAP2 involved in anthocyanin accumulation in Arabidopsis.
Alexander Maier,Andrea Schrader,Leonie Kokkelink,Christian Falke,Bastian Welter,Elisa Iniesto,Vicente Rubio,Joachim F. Uhrig,Martin Hülskamp,Ute Hoecker +9 more
Reads0
Chats0
TLDR
These findings have identified mechanisms via which the COP1/SPA complex controls anthocyanin levels in Arabidopsis that may be useful for applications in biotechnology directed towards increasing anthocianin content in plants.Abstract:
Summary
Anthocyanins are natural pigments that accumulate only in light-grown and not in dark-grown Arabidopsis plants. Repression of anthocyanin accumulation in darkness requires the CONSTITUTIVELY PHOTOMORPHOGENIC1/SUPPRESSOR OF PHYA-105 (COP1/SPA) ubiquitin ligase, as cop1 and spa mutants produce anthocyanins also in the dark. Here, we show that COP1 and SPA proteins interact with the myeloblastosis (MYB) transcription factors PRODUCTION OF ANTHOCYANIN PIGMENT1 (PAP)1 and PAP2, two members of a small protein family that is required for anthocyanin accumulation and for the expression of structural genes in the anthocyanin biosynthesis pathway. The increased anthocyanin levels in cop1 mutants requires the PAP1 gene family, indicating that COP1 functions upstream of the PAP1 gene family. PAP1 and PAP2 proteins are degraded in the dark and this degradation is dependent on the proteasome and on COP1. Hence, the light requirement for anthocyanin biosynthesis results, at least in part, from the light-mediated stabilization of PAP1 and PAP2. Consistent with this conclusion, moderate overexpression of PAP1 leads to an increase in anthocyanin levels only in the light and not in darkness. Here we show that SPA genes are also required for reducing PAP1 and PAP2 transcript levels in dark-grown seedlings. Taken together, these results indicate that the COP1/SPA complex affects PAP1 and PAP2 both transcriptionally and post-translationally. Thus, our findings have identified mechanisms via which the COP1/SPA complex controls anthocyanin levels in Arabidopsis that may be useful for applications in biotechnology directed towards increasing anthocyanin content in plants.read more
Citations
More filters
Journal ArticleDOI
Transcriptional control of flavonoid biosynthesis by MYB–bHLH–WDR complexes
TL;DR: Recent advances in the characterization of the underlying regulatory mechanisms of flavonoid biosynthesis are reviewed, with a special focus on the MBW (MYB-bHLH-WDR) protein complexes.
Journal ArticleDOI
MYB Transcription Factors as Regulators of Phenylpropanoid Metabolism in Plants
TL;DR: This review summarizes the current understanding of V-myb myeloblastosis viral oncogene homolog (MYB) proteins and their roles in the regulation of phenylpropanoid metabolism in plants.
Journal ArticleDOI
A Conserved Network of Transcriptional Activators and Repressors Regulates Anthocyanin Pigmentation in Eudicots
Nick W. Albert,Nick W. Albert,Nick W. Albert,Kevin M. Davies,David H. Lewis,Huaibi Zhang,Mirco Montefiori,Cyril Brendolise,Murray R. Boase,Hanh Ngo,Paula E. Jameson,Kathy E. Schwinn +11 more
TL;DR: The gene regulatory network that regulates the timing, location, and level of anthocyanin pigmentation in petunia is characterized, establishing features of the network that are functionally conserved in eudicots and are thus likely to be widespread in the Eudicots.
Journal ArticleDOI
Light-controlled flavonoid biosynthesis in fruits.
TL;DR: An overview of the currently known mechanisms of the light-controlled flavonoid accumulation is provided and R2R3 MYB transcription factors are known to regulate by differential expression the biosynthesis of distinct flavonoids in response to specific light wavelengths.
Journal ArticleDOI
Molecular genetics of blood-fleshed peach reveals activation of anthocyanin biosynthesis by NAC transcription factors.
Hui Zhou,Kui Lin-Wang,Huiliang Wang,Chao Gu,Andrew P. Dare,Richard V. Espley,Huaping He,Andrew C. Allan,Yuepeng Han +8 more
TL;DR: A mechanism whereby BL is the key gene for the blood-flesh trait in peach via its activation of PpMYB10.1 is presented, resulting in anthocyanin pigmentation in tobacco.
References
More filters
Journal ArticleDOI
Environmental Significance of Anthocyanins in Plant Stress Responses
TL;DR: The environmental induction of anthocyanins and their proposed importance in ameliorating environmental stresses induced by visible and UVB radiation, drought and cold temperatures are reviewed.
Journal ArticleDOI
Flavonoids: a colorful model for the regulation and evolution of biochemical pathways
TL;DR: The analysis of pigmentation continues to provide insights into new areas, such as the channeling and intracellular transport of metabolites, regulation of gene expression and RNA interference.
Journal ArticleDOI
Activation Tagging Identifies a Conserved MYB Regulator of Phenylpropanoid Biosynthesis
TL;DR: A novel approach for enhancing the accumulation of natural products based on activation tagging by Agrobacterium-mediated transformation with a T-DNA that carries cauliflower mosaic virus 35S enhancer sequences at its right border is reported.
Journal ArticleDOI
Regulation of the anthocyanin biosynthetic pathway by the TTG1/bHLH/Myb transcriptional complex in Arabidopsis seedlings.
TL;DR: It is demonstrated that overexpression of Myb113 or Myb114 results in substantial increases in pigment production similar to those previously seen as a result of over-expression of PAP1, and pigment production in these overexpressors remains TTG1- and bHLH-dependent.
Journal ArticleDOI
Targeted destabilization of HY5 during light-regulated development of Arabidopsis
TL;DR: It is shown that the abundance of HY5 is directly correlated with the extent of photomorphogenic development, and that the COP1–HY5 interaction may specifically target HY5 for proteasome-mediated degradation in the nucleus.