Journal ArticleDOI
Lipase catalysed hydrolysis of ricebran oil by free and immobilised enzyme system in batch stirred reactor
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A change of the reaction rate was observed for the lipasecatalysed hydrolysis of ricebran oil in a batch stirred tank reactor using immobilized lipase enzyme as compared to free enzyme.Abstract:
A change of the reaction rate was observed for the lipasecatalysed hydrolysis of ricebran oil in a batch stirred tank reactor using immobilized lipase enzyme as compared to free enzyme. The reactor rate was observed to be controlled mainly by factors like temperature, pH, initial enzyme concentration, initial substrate concentration and initial products concentration.read more
Citations
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Journal ArticleDOI
Lipase applications in oil hydrolysis with a case study on castor oil: a review
TL;DR: In this article, a single variable optimization method is used to obtain optimum conditions for a lipid-catalyzed process of vegetable oil hydrolysis, where the presence of metal ions has different effects on the activity of different lipases and the effects of additives on the same lipase vary with their types.
Journal ArticleDOI
Hydrolysis of rice bran oil using immobilized lipase in a stirred batch reactor
TL;DR: Candida cylindracea lipase was immobilized by adsorption on acid washed glass beads and it was observed that protein loading of the support depends on the size of the particle, with smaller particle containing higher amount of protein per unit weight.
Journal ArticleDOI
Kinetics of enzymatic hydrolysis of rice bran oil in organic system
TL;DR: In this paper, the kinetics of enzymatic hydrolysis of rice bran oil in organic solvent (n -hexane) by free and immobilized Candida lipolytica lipase were investigated in a batch reactor.
References
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Book
Biochemical engineering fundamentals
James E. Bailey,David F. Ollis +1 more
TL;DR: Biochemical Engineering Fundamentals, 2/e as mentioned in this paper combines contemporary engineering science with relevant biological concepts in a comprehensive introduction to biochemical engineering, which enables students to comprehend the major problems in biochemical engineering and formulate effective solutions.
Journal ArticleDOI
A simple and rapid colorimetric method for determination of free fatty acids for lipase assay
Dae Y. Kwon,Joon Shick Rhee +1 more
TL;DR: A simple and rapid colorimetric method was developed to determine the lipase activity for fat splitting by observing the color developed using cupric acetate-pyridine as a color developing reagent.
Journal ArticleDOI
Characteristics of lipase‐catalyzed hydrolysis of olive oil in AOT–isooctane reversed micelles
Daeseok Han,Joon Shick Rhee +1 more
TL;DR: Candida rugosa lipase solubilized in organic solvents in the presence of both surfactant and water could catalyze the hydrolysis of triglycerides, and kinetic analysis of the lipase‐catalyzed reaction was found to be possible in this system.
Journal ArticleDOI
Continuous hydrolysis of olive oil by lipase in microporous hydrophobic membrane bioreactor
TL;DR: The hydrolysis seemed to be limited by diffusion of fat or fatty acid through the micropores of the membrane at higher interfacial enzyme concentrations, and the lipase was stabilized significantly by glycerol added to the buffer solution.
Journal ArticleDOI
Application of immobilized lipase to hydrolysis of triacylglyceride
TL;DR: Lipase from Candida cylindracea was immobilized by entrapment with photo-crosslinkable resin prepolymers or urethane prepolymer, and by covalent binding or by adsorption to different types of porous inorganic or organic supports to show some activity for hydrolysis of olive oil.
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