Lipid changes of goat sperm plasma membrane during epididymal maturation
TL;DR: The altered lipid profile of the mature sperm membrane leads to changes in its fluidity that play an important role in determining the structure and functions of the biomembrane.
About: This article is published in Biochimica et Biophysica Acta.The article was published on 1991-01-30. It has received 76 citations till now. The article focuses on the topics: Sperm plasma membrane & Membrane lipids.
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10 Sep 2015
TL;DR: The aim of this review is to critically examine and summarize the data gathered during the past few years from both experimental animal models and human studies on the relation between hypercholesterolemia and semen parameters, endocrine status, spermatogenesis and male fertility.
Abstract: Hyper-Lipidemia/hypercholesterolemia is a major health problem all over the world and it is emerging as an important cause of adverse health outcomes including cardiovascular complications, metabolic disorders and infertility. Over the years, many population based studies have highlighted a trend towards deterioration of semen quality and decline of male fertility. The rise in worldwide dyslipidaemia combined with the trend of decreasing semen quality and male fertility, has called an attention of scientific community. A large number of research papers have been published to explore the links between hyperlipidaemia and male infertility. The aim of this review is to critically examine and summarize the data gathered during the past few years from both experimental animal models and human studies on the relation between hypercholesterolemia and semen parameters, endocrine status, spermatogenesis and male fertility. For this purpose, the PubMed, Scopus and Google Scholar databases were comprehensively searched with the help of various search terms. Experimental studies based on hypercholetrolemic/high fat diet fed animal models and studies conducted on normal infertile/obese infertile men clearly demonstrate a negative impact of hypercholesterolemia on testicular functions, reproductive hormone synthesis and secretion, sperm maturation, sperm quality parameters and ejaculatory functions leading to male infertility. Various mechanisms have been suggested for such actions.
17 citations
Cites background from "Lipid changes of goat sperm plasma ..."
...A significant (50%) decrease in the level of sperm cholesterol has been reported in hamster [20], mouse [21], and rat [22] and ram [23] whereas no significant change in sperm cholesterol content was observed in boar [24] and a significant increase was observed in goat [25]....
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TL;DR: Results are promising, but studies to optimize the protocols for freezing sperm directly from room temperature need to be conducted, as well as studies to determine how cryopreserving sperm in this manner affects other sperm functions.
Abstract: Cholesterol-loaded cyclodextrins (CLC) added to the sperm before cryopreservation enhance sperm quality after freeze-thawing in several cold shock-sensitive species, including cattle and goats. However, all studies conducted to date have used conventional protocols, in which sperm are cooled slowly to 5°C before freezing. As cholesterol plays a significant role in sperm cold shock resistance, it is possible that CLC-treated sperm can withstand cooling damage when the sperm are not cooled slowly to 5°C before freezing. In this study, we determined whether CLC-treated goat (1 mg CLC/120×106 sperm) and bull (2 mg CLC/120×106 sperm) sperm quality, after thawing, was different for sperm frozen using conventional protocols (including a slow cooling phase to 5oC) and protocols in which the sperm were frozen from room temperature, without cooling the sperm slowly to 5°C before freezing. CLC-treated sperm exhibited higher percentages of plasma membrane-intact sperm than control sperm when cryopreserved using conventional protocols. In addition, CLC treatment enhanced both sperm motility and plasma membrane integrity when sperm were frozen directly from room temperature. However, this treatment did not fully prevent the damage of the sperm after cooling rapidly and subsequent freezing, as the sperm quality was lower than that presented by the samples frozen using the conventional protocol. The results are promising, but studies to optimize the protocols for freezing sperm directly from room temperature need to be conducted, as well as studies to determine how cryopreserving sperm in this manner affects other sperm functions.
14 citations
Cites background from "Lipid changes of goat sperm plasma ..."
...Goat sperm plasma membranes contain more phosphatidylethanolamine (Rana et al., 1991) than bull sperm membranes (Watson, 1981), and phosphatidylethanolamine is a lipid that aligns into the hexagonal-II phase orientation when cooled....
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...Goat sperm plasma membranes contain more phosphatidylethanolamine (Rana et al., 1991) than bull sperm membranes (Watson, 1981), and phosphatidylethanolamine is a lipid that aligns into the hexagonal-II phase orientation when cooled....
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TL;DR: The authors' data confirmed that CLC treatment improves ram sperm cold shock and osmotic stress resistance, and augments sperm cholesterol content, and confirms that cholesterol is incorporated into the sperm using CLC.
13 citations
TL;DR: Findings indicate that ether‐linked phospholipids activate PI‐3‐kinase and may participate in mitogenic responses, as compared to vertebrate sources.
Abstract: Phosphatidylcholine (PC) from marine invertebrates is enriched in ether-linked forms. PCs from ray fish, Dasyatis sp., and bivalve, Macoma birmanica, used in the present study, contain 65% and 75% (w/w of total PC) of ether-linked forms, respectively. Ether-linked PCs also occur in mammalian membranes. Agonist-mediated hydrolysis of PC generates second messengers which participate in cellular responses. In this study, we tested whether PCs from marine invertebrates directly affect mammalian cell growth and activity of phosphatidylinositol (Pl-3-kinase). Pl-3-kinase participates in mitogenesis initiated by a variety of growth factors. Pl-3-kinase converts polyphosphoinositides to 3' phosphorylated isomers and these products accumulate in response to mitogenic stimuli. Whether cell membrane lipids regulate Pl-3-kinase activity is not known. The marine animal-derived PCs and dioleoyl DAG (dioleoylglycerol) stimulated growth of murine pre-B lymphocytes, whereas chicken PC (egg lecithin) inhibited growth of these cells. Egg lecithin is also a potent inhibitor of Pl-3-kinase activity in vitro. We studied the effect of PCs and DAG on Pl-3-kinase activity. Unlike egg lecithin, marine animal PCs enhanced Pl-3-kinase activity. We investigated the effect of lipids on Pl-3-kinase substrate utilization. PCs enriched in ether-linked species increased utilization of substrates by Pl-3-kinase. PCs purified from marine organisms also contain a substantially higher percentage of the cis-unsaturated fatty acids, especially of the -omega 3 series (25% and 30% of total fatty acids for Dasyatis sp. and Macoma birmanica, respectively), as compared to vertebrate sources. In spite of differences in fatty acid composition, marine PCs and dioleoyl DAG showed similar effects on cell growth and Pl-3-kinase activity. These findings indicate that ether-linked phospholipids activate Pl-3-kinase and may participate in mitogenic responses.
12 citations
TL;DR: The use of the chicken EY (C) diluent resulted in better post-thawing buck semen quality, including the total progressive sperm motility parameters, sperm vitality, plasma membrane intactness, DNA integrity, and sperm abnormalities percentages, in comparison with the EY diluents of the other avian species.
11 citations
References
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