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Journal ArticleDOI

Lipid changes of goat sperm plasma membrane during epididymal maturation

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TLDR
The altered lipid profile of the mature sperm membrane leads to changes in its fluidity that play an important role in determining the structure and functions of the biomembrane.
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This article is published in Biochimica et Biophysica Acta.The article was published on 1991-01-30. It has received 76 citations till now. The article focuses on the topics: Sperm plasma membrane & Membrane lipids.

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Polyunsaturated Fatty Acids in Male Ruminant Reproduction — A Review

TL;DR: Four aspects of PUFA are reviewed in light of previous research including rumen metabolism, biological roles, influence on reproduction, and strategies to use in male ruminants.
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Deterioration of goat sperm viability in milk extenders is due to a bulbourethral 60-kilodalton glycoprotein with triglyceride lipase activity.

TL;DR: The preliminary identification of the goat BUSgp60 as a bulbourethral lipase belonging to the PL-RP2 family is supported, for the best preservation of goat semen, specific inhibitors of this family of lipase could be added into milk-based extenders without eliminating seminal plasma.
Journal ArticleDOI

Epididymis cholesterol homeostasis and sperm fertilizing ability.

TL;DR: A review of data reported showing how imbalanced cholesterol levels may particularly affect the post-testicular events of sperm maturation that lead to fully fertile male gametes is focused on.
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Incorporating Lipids into Boar Sperm Decreases Chilling Sensitivity but Not Capacitation Potential

TL;DR: Liposomes of complex composition can fuse to boar sperm without harming in vitro capacitation or acrosome reaction and reduce sperm chilling sensitivity.
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Uncovering sperm metabolome to discover biomarkers for bull fertility

TL;DR: This is the first study aimed at ascertaining the metabolome of spermatozoa from bulls with different fertility phenotype using gas chromatography-mass spectrometry and identified five metabolites in the two groups of sires that can be used, in the future, as key indicators of bull fertility.
References
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Journal Article

Protein Measurement with the Folin Phenol Reagent

TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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A simple method for the isolation and purification of total lipides from animal tissues.

TL;DR: In this paper, the authors described a simplified version of the method and reported the results of a study of its application to different tissues, including the efficiency of the washing procedure in terms of the removal from tissue lipides of some non-lipide substances of special biochemical interest.
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Two dimensional thin layer chromatographic separation of polar lipids and determination of phospholipids by phosphorus analysis of spots

TL;DR: Separation of polar lipids by two-dimensional thin layer chromatography providing resolution of all the lipid classes commonly encountered in animal cells and a sensitive, rapid, reproducible procedure for determination of phospholipids by phosphorus analysis of spots are described.
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