Journal ArticleDOI
Lipid changes of goat sperm plasma membrane during epididymal maturation
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TLDR
The altered lipid profile of the mature sperm membrane leads to changes in its fluidity that play an important role in determining the structure and functions of the biomembrane.About:
This article is published in Biochimica et Biophysica Acta.The article was published on 1991-01-30. It has received 76 citations till now. The article focuses on the topics: Sperm plasma membrane & Membrane lipids.read more
Citations
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Journal ArticleDOI
Lipid diffusion in the plasma membrane of ram and boar spermatozoa during Maturation in the epididymis measured by fluorescence recovery after photobleaching
TL;DR: The technique of fluorescence recovery after photobleaching (FRAP) is used to measure diffusion of the lipid reporter probe ODAF in spermatozoa from two species: ram and boar, where substantial changes in membrane lipids occur during passage through the epididymis, and there are relatively few changes.
Journal ArticleDOI
Effects of metabolic rate and sperm competition on the fatty-acid composition of mammalian sperm
TL;DR: A comparative study in mammals found that high MSMR and high levels of sperm competition both promote a decrease in the proportion of PUFAs that are more prone to lipid peroxidation, which supports the idea that the effects of MS MR and sperm competition on sperm function can operate at very different levels.
Journal ArticleDOI
Uneven Distribution of Ceramides, Sphingomyelins and Glycerophospholipids Between Heads and Tails of Rat Spermatozoa
TL;DR: The fact that intact rat spermatozoa constitutively have much more VLCPUFA-containing ceramide than sphingomyelin is explained by the present findings, since the former are mostly lipids of the large tail while the latter mostly collect in the small head.
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Optimizing and quantifying fusion of liposomes to mammalian sperm using resonance energy transfer and flow cytometric methods.
TL;DR: Liposome fusion with mammalian sperm membranes can be quantified cytometrically and varies with lipid composition, sperm-to-liposome ratio, and individual animals.
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Membrane fluidity changes in goat sperm induced by cholesterol depletion using beta-cyclodextrin.
TL;DR: After cholesterol removal from mature goat spermatozoa, important changes in the relative anisotropy range of the two transitions were found, indicating an increase in the "fluidity" of some of the lipid microdomains of sperm membranes.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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A simple method for the isolation and purification of total lipides from animal tissues.
TL;DR: In this paper, the authors described a simplified version of the method and reported the results of a study of its application to different tissues, including the efficiency of the washing procedure in terms of the removal from tissue lipides of some non-lipide substances of special biochemical interest.
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Two dimensional thin layer chromatographic separation of polar lipids and determination of phospholipids by phosphorus analysis of spots
TL;DR: Separation of polar lipids by two-dimensional thin layer chromatography providing resolution of all the lipid classes commonly encountered in animal cells and a sensitive, rapid, reproducible procedure for determination of phospholipids by phosphorus analysis of spots are described.
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