Journal ArticleDOI
Lipid changes of goat sperm plasma membrane during epididymal maturation
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TLDR
The altered lipid profile of the mature sperm membrane leads to changes in its fluidity that play an important role in determining the structure and functions of the biomembrane.About:
This article is published in Biochimica et Biophysica Acta.The article was published on 1991-01-30. It has received 76 citations till now. The article focuses on the topics: Sperm plasma membrane & Membrane lipids.read more
Citations
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Journal ArticleDOI
Targeted disruption of hormone-sensitive lipase results in male sterility and adipocyte hypertrophy, but not in obesity
Jun-ichi Osuga,Shun Ishibashi,Teruaki Oka,Hiroaki Yagyu,Ryu-ichi Tozawa,Akihisa Fujimoto,Futoshi Shionoiri,Naoya Yahagi,Frederic B. Kraemer,Osamu Tsutsumi,Nobuhiro Yamada +10 more
TL;DR: It is shown that HSL is required for spermatogenesis but is not the only enzyme that mediates the hydrolysis of triacylglycerol stored in adipocytes, and that adipocytes were significantly enlarged and the size differences between cell and tissue suggests the heterogeneity of adipocytes.
Journal ArticleDOI
Bicarbonate stimulated phospholipid scrambling induces cholesterol redistribution and enables cholesterol depletion in the sperm plasma membrane
Frits M. Flesch,Jos F. Brouwers,Patricia F. E. M. Nievelstein,Arie J. Verkleij,Lambert M.G. van Golde,Ben Colenbrander,Barend M. Gadella +6 more
TL;DR: A model is proposed in which phospholipid scrambling induces the formation of an apical membrane raft in the sperm head surface that enables albumin mediated efflux of cholesterol.
Journal ArticleDOI
A porcine homolog of the major secretory protein of human epididymis, HE1, specifically binds cholesterol.
Naomichi Okamura,Sachiko Kiuchi,Michiko Tamba,Takayuki Kashima,Shinsuke Hiramoto,Tadashi Baba,Françoise Dacheux,Jean-Louis Dacheux,Yoshiki Sugita,Yin-Zhe Jin +9 more
TL;DR: It was found that the He1 homolog is a major cholesterol-binding protein in the porcine epididymal fluid and the possibility that the HE1 homology is involved in the regulation of the lipid composition of the sperm membranes during the maturation in epidIDymis is discussed.
Journal ArticleDOI
The epididymis and sperm maturation: a perspective.
TL;DR: In common mammals, sperm leaving the testis are incapable of fertilizing a female gamete, and when assessing sperm maturation it is necessary to establish the proportion of sperm that has completed and retained all steps of maturation necessary to achieve fertilization of oocytes under the conditions imposed.
References
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Journal ArticleDOI
Changes in phospholipids of ram spermatozoa during migration through the epididymis and possible origin of prostaglandin F2α in testicular and epididymal fluid
TL;DR: Of the phospholipid-bound fatty acids the most pronounced change occurs in palmitic during migration of spermatozoa through the reproductive tract, and prompted a study of the prostaglandin content of testicular and epididymal fluids, since arachidonic acid can act as a precursor of prostaglandsin.
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Biochemical parameters of initiation and regulation of sperm motility.
TL;DR: Several studies suggest that exogenous FMP in concert with elevated intrasperm pH and level of cAMP initiates flagellar motility during the epididymal transit of sperm.
Journal ArticleDOI
Two-dimensional reaction thin-layer chromatography in the analysis of phosphatide plasmalogens.
TL;DR: A two-dimensional reaction thin-layer chromatography procedure on micro- samples of mixtures of alkenyl acyl and diacyl phosphatides has been described by which it is possible to determine simultaneously their plasmalogen content, the fatty acid composition of invidual analogs and the fatty aldehyde composition of the alkenYL acyl analog.
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Systematic identification of fatty acids in the gas-liquid chromatography of fatty acid methyl esters: a preliminary survey of seal oil.
TL;DR: The fatty acids of a commercial seal blubber oil have been surveyed by application of the linear log plot and separation factor procedures to the gas–liquid chromatograms of methyl esters obtained from solvent-free gas and liquid chromatography.
Journal ArticleDOI
Isolation of plasma membrane from porcine spermatozoa.
TL;DR: Porcine spermatozoa were separated from gel particles and seminal plasma and subjected to low-intensity sonication that fragmented the plasma membrane, but minimized damage to the acrosome, nucleus and mitochondria.
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