Lipid composition and thermotropic phase behavior of boar, bull, stallion, and rooster sperm membranes.
TL;DR: A difference in both composition and thermotropic phase behavior of glycolipids between rooster and mammalian sperm is demonstrated which may be related to the greater tolerance of rooster sperm to rapid cooling.
About: This article is published in Cryobiology.The article was published on 1992-04-01. It has received 486 citations till now. The article focuses on the topics: Sperm plasma membrane & Sperm.
Citations
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TL;DR: The possible roles of cryoprotectants and additives are considered in the context of their putative interactions with the sperm plasma membrane and modern approaches to the laboratory assessment of spermatozoa after freeze-thawing are discussed.
648 citations
Cites background from "Lipid composition and thermotropic ..."
...Spermatozoa undergo these lipid phase transitions Crowe et .al., 1989; Drobnis et al., 1993; Holt and North, 1984; 1986; Parks and Lynch, 1992 typically within the temperature range 17–368C....
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TL;DR: Dynamics in adhesive and fusion properties, molecular composition and architecture of the sperm plasma membrane, as well as membrane derived signalling are reviewed.
580 citations
TL;DR: In this paper, the complexity of the sperm plasma membrane and the many steps in a cryopreservation procedure where membrane perturbations can occur are described, and an attempt has been made to describe the complexities of the SPM and its interaction with its components.
422 citations
407 citations
TL;DR: Catalase (CAT) activities were higher in the group that was applied 25mM taurine as an antioxidant, than in all of the other groups in this study, and the addition of antioxidants did not cause significant differences in levels of malondialdehyde (MDA), glutathione (GSH), and glutATHione peroxidase (G SH-Px), after thawing, when compared to groups with no additives.
308 citations
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TL;DR: In this paper, the authors described a simplified version of the method and reported the results of a study of its application to different tissues, including the efficiency of the washing procedure in terms of the removal from tissue lipides of some non-lipide substances of special biochemical interest.
59,550 citations
7,125 citations
TL;DR: The original Lowry method of protein determination has been modified by the addition of sodium dodecyl sulfate in the alkali reagent and an increase in the amount of copper tartrate reagent to be used with membrane and lipoprotein preparations without prior solubilization or lipid extraction.
6,336 citations
TL;DR: The cations of T4 phage have been examined and a balance has been obtained between total cations and total DNA anions and the replacement of the normal polyamines suggested that the polyamines may be acting as nonspecific cations.
2,468 citations