Lipid extraction of tissues with a low-toxicity solvent
Atsushi Hara,Norman S. Radin +1 more
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TLDR
An improved method for extracting the lipids from tissues consists of the use of hexane:isopropanol, followed by a wash of the extract with aqueous sodium sulfate to remove nonlipid contaminants.About:
This article is published in Analytical Biochemistry.The article was published on 1978-10-01 and is currently open access. It has received 2400 citations till now. The article focuses on the topics: Elution & Extraction (chemistry).read more
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Nile red: a selective fluorescent stain for intracellular lipid droplets.
TL;DR: The dye nile red, 9-diethylamino-5H- benzo[alpha]phenoxazine-5-one, is an excellent vital stain for the detection of intracellular lipid droplets by fluorescence microscopy and flow cytofluorometry and it exhibits properties of a near-ideal lysochrome.
Journal ArticleDOI
Adipocyte-Derived Plasma Protein, Adiponectin, Suppresses Lipid Accumulation and Class A Scavenger Receptor Expression in Human Monocyte-Derived Macrophages
Noriyuki Ouchi,Shinji Kihara,Yukio Arita,Makoto Nishida,Akifumi Matsuyama,Yoshihisa Okamoto,Masato Ishigami,Hiroshi Kuriyama,Ken Kishida,Hitoshi Nishizawa,Kikuko Hotta,Masahiro Muraguchi,Yasukazu Ohmoto,Shizuya Yamashita,Tohru Funahashi,Yuji Matsuzawa +15 more
TL;DR: The adipocyte-derived plasma protein adiponectin suppressed macrophage-to-foam cell transformation, suggesting that adip onectin may act as a modulator for macrophages- to-foams cell transformation.
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Acyl-Lipid Metabolism
Younghua Li-Beisson,Basil S. Shorrosh,Fred Beisson,Mats X. Andersson,Vincent Arondel,Philip D. Bates,Sébastien Baud,David Bird,Debono Allan George,Timothy P. Durrett,Rochus Franke,Ian A. Graham,Kenta Katayama,Amélie A. Kelly,Tony R. Larson,Jonathan E. Markham,Martine Miquel,Isabel Molina,Ikuo Nishida,Owen Rowland,Lacey Samuels,Katherine M. Schmid,Hajime Wada,Ruth Welti,Changcheng Xu,Rémi Zallot,John B. Ohlrogge +26 more
TL;DR: This chapter focuses on the metabolic pathways that are associated with the biosynthesis and degradation of the acyl lipids that represent their major form of carbon and energy storage in Arabidopsis.
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Conjugated linoleic acid is synthesized endogenously in lactating dairy cows by Delta(9)-desaturase.
J. M. Griinari,Benjamin A. Corl,Shannon H. Lacy,P. Y. Chouinard,K. V. V. Nurmela,Dale E. Bauman +5 more
TL;DR: Results demonstrate that endogenous synthesis of CLA from trans-11 18:1 represented the primary source of CLA in milk fat of lactating cows.
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The cholesteryl ester cycle in macrophage foam cells. Continual hydrolysis and re-esterification of cytoplasmic cholesteryl esters.
TL;DR: Investigation of the metabolism of mouse peritoneal macrophages with acetylated human low density lipoprotein and following the fate of the cytoplasmic cholesteryl esters demonstrates that these esters undergo a continual cycle of hydrolysis and re-esterification with a half-life of about 24 h.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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A simple method for the isolation and purification of total lipides from animal tissues.
TL;DR: In this paper, the authors described a simplified version of the method and reported the results of a study of its application to different tissues, including the efficiency of the washing procedure in terms of the removal from tissue lipides of some non-lipide substances of special biochemical interest.
Journal ArticleDOI
A rapid method of total lipid extraction and purification.
E. G. Bligh,W. J. Dyer +1 more
TL;DR: The lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials that has been applied to fish muscle and may easily be adapted to use with other tissues.